Tissue-engineered acellular small diameter long-bypass grafts with neointima-inducing activity

Mahara, Atsushi; Somekawa, Shota; Kobayashi, Naoki; Hirano, Yoshiaki; Kimura, Yoshiharu; Fujisato, Toshiya; Yamaoka, Tetsuji

doi:10.1016/j.biomaterials.2015.04.031

Cited by 131 publications

(135 citation statements)

References 28 publications

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“…For this reason, the use of these peptides for therapeutic applications in vivo is limited due to the possibility of the recruitment of undesired cell types, which could cause further complications or undesired tissue growth in the site of injury. Therefore, an increasingly popular research focus is the identification of novel adhesive peptides that selectively bind to single cell types such as EPCs [125, 126]. …”

Section: Cardiovascular Regeneration Using Stem Cell Recruitment Smentioning

confidence: 99%

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Pacelli

Basu

Whitlow

et al. 2017

Advanced Drug Delivery Reviews

121

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A leading strategy in tissue engineering is the design of biomimetic scaffolds that stimulate the body’s repair mechanisms through the recruitment of endogenous stem cells to sites of injury. Approaches that employ the use of chemoattractant gradients to guide tissue regeneration without external cell sources are favored over traditional cell-based therapies that have limited potential for clinical translation. Following this concept, bioactive scaffolds can be engineered to provide a temporally and spatially controlled release of biological cues, with the possibility to mimic the complex signaling patterns of endogenous tissue regeneration. Another effective way to regulate stem cell activity is to leverage the inherent chemotactic properties of extracellular matrix (ECM)-based materials to build versatile cell-instructive platforms. This review introduces the concept of endogenous stem cell recruitment, and provides a comprehensive overview of the strategies available to achieve effective cardiovascular and bone tissue regeneration.

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Section: Cardiovascular Regeneration Using Stem Cell Recruitment Smentioning

confidence: 99%

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Pacelli

Basu

Whitlow

et al. 2017

Advanced Drug Delivery Reviews

121

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“…Others have previously pointed to the relative advantages of decellularized arterial ECMs as they inherently preserve the native artery ultrastructure and related mechanical properties (e.g., burst pressure), while avoiding the immunogenicity of donor cells. 4,24,25 In fact, several studies already showed that decellularized porcine iliac and carotid vessels may be ideal source material and that following endothelialization, these grafts remained patent for up to 130 days. 24,26 However, different ECM compositions may be expected from different decellularization procedures, 27,28 hence the product of various decellularization processes may be different between groups and care should be taken when comparing these results.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Autologous Reendothelialization of Small-Caliber Arterial Extracellular Matrix: A Preclinical Large Animal Study

Dahan

Sarig

Bronshtein

et al. 2017

Tissue Engineering Part A

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Effective cellularization is a key approach to prevent small-caliber (<4 mm) tissue-engineered vascular graft (TEVG) failure and maintain patency and contractility following implantation. To achieve this goal, however, improved biomimicking designs and/or relatively long production times (typically several months) are required. We previously reported on porcine carotid artery decellularization yielding biomechanically stable and cell supportive small-caliber (3–4 mm diameter, 5 cm long) arterial extracellular matrix (scaECM) vascular grafts. In this study, we aimed to study the scaECM graft patency in vivo and possibly improve that patency by graft pre-endothelialization with the recipient porcine autologous cells using our previously reported custom-designed dynamic perfusion bioreactor system. Decellularized scaECM vascular grafts were histologically characterized, their immunoreactivity studied in vitro, and their biocompatibility profile evaluated as a xenograft subcutaneous implantation in a mouse model. To study the scaECM cell support and remodeling ability, pig autologous endothelial and smooth muscle cells (SMCs) were seeded and dynamically cultivated within the scaECM lumen and externa/media, respectively. Finally, endothelialized-only scaECMs—hypothesized as a prerequisite for maintaining graft patency and controlling intimal hyperplasia—were transplanted as an interposition carotid artery graft in a porcine model. Graft patency was evaluated through angiography online and endpoint pathological assessment for up to 6 weeks. Our results demonstrate the scaECM-TEVG biocompatibility preserving a structurally and mechanically stable vascular wall not just following decellularization and recellularization but also after implantation. Using our dynamic perfusion bioreactor, we successfully demonstrated the ability of this TEVG to support in vitro recellularization and remodeling by primary autologous endothelial and SMCs, which were seeded on the lumen and the externa/media layers, respectively. Following transplantation, dynamically endothelialized scaECM-TEVGs remained patent for 6 weeks in a pig carotid interposition bypass model. When compared with nonrevitalized control grafts, reendothelialized grafts provided excellent antithrombogenic activity, inhibited intimal hyperplasia formation, and encouraged media wall infiltration and reorganization with recruited host SMCs. We thus demonstrate that readily available decellularized scaECM can be promptly revitalized with autologous cells in a 3-week period before implantation, indicating applicability as a future platform for vascular reconstructive procedures.

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“…Physical methods Break the cells Sonication/ultrahigh-hydrostatic pressure/freezing-thawing: Accelerate and improve the efficiency of cell-lysing agents, but damages both collagen and elastin fibers and alter their mechanical properties (Azhim, Yamagami, Muramatsu, Morimoto, & Tanaka, 2011;Funamoto et al, 2010;Mahara et al, 2015) Chemical properties and better cell removal efficiency than non-ionic detergents (Daugs et al, 2017) Enzyme digestion Break the binding of cells, specifically digest cell components…”

Section: Mechanism Common Reagents and Methodsmentioning

confidence: 99%

Strategies in cell‐free tissue‐engineered vascular grafts

Yuan

Chen

Liu

et al. 2019

J Biomedical Materials Res

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Cardiovascular diseases (CVD) remain the leading cause of morbidity and mortality in the world, among which coronary artery diseases (CAD) are the most common type of CVD. Coronary artery bypass grafting (CABG) using autologous vein and artery grafts is the typical surgical intervention for CAD patients. However, for patients whose autologous grafts are not available, there are no appropriate substitutes for vascular grafts. Investigation of tissue-engineered vascular graft (TEVG) has persisted over decades with significant advancement, utilizing different types of biomaterials.In the past two decades, a great number of studies based on cell-seeding strategies were reported. However, limitations of cell-based strategies made clinical application difficult. With the understanding of stem cells and tissue remodeling process, strategies without cell-seeding emerged as potential methods to achieve in situ regeneration.A cell-free graft may recruit host cells and guide their participation in vascular remodeling. The grafts modified by bio-active molecules showed good results in promoting in situ regeneration and exhibited potential to make the vascular grafts off-the-shelf.In this review, the strategies for cell-free TEVG manufacturing were discussed, including the materials for fabricating TEVGs, the methods of functionalization to promote in situ regeneration, the challenges researchers faced in TEVG investigation, and finally the prospects in TEVG design.

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Tissue-engineered acellular small diameter long-bypass grafts with neointima-inducing activity

Cited by 131 publications

References 28 publications

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Dynamic Autologous Reendothelialization of Small-Caliber Arterial Extracellular Matrix: A Preclinical Large Animal Study

Strategies in cell‐free tissue‐engineered vascular grafts

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