Tissue distribution of anthocyanins in rats fed a blackberry anthocyanin‐enriched diet

Abstract

Anthocyanins are natural dietary pigments that could be involved in various health effects. The aim of this study was to investigate the distribution of anthocyanins to various organs (bladder, prostate, testes, heart and adipose tissue) in rats fed with a blackberry anthocyanin-enriched diet for 12 days. Identification and quantification of anthocyanins were carried out by HPLC-DAD. The urinary excretion of total anthocyanins (native anthocyanins and their metabolites) was low (0.20 +/- 0.03%, n = 8). Proportions of anthocyanin derivatives (methylated anthocyanins and glucurono-conjugated derivatives) differed according to the organ considered. The bladder contained the highest levels of anthocyanins followed by the prostate. Prostate, testes and heart contained native cyanidin 3-glucoside and a small proportion of cyanidin monoglucuronide. Cyanidin 3-glucoside and methylated derivatives were present in adipose tissue. Thus, anthocyanin feeding in rats resulted in a wide distribution of anthocyanin derivatives to several organs. Identification of target tissues of anthocyanins may then help to understand the mechanisms of action of anthocyanins in vivo.

Anthocyanins and human health: How gastric absorption may influence acute human physiology

“…In a more recent work, proportions of anthocyanin derivatives (methylated anthocyanins and glucuronoconjugated derivatives) were identified in various organs (bladder, prostate, testes, heart and adipose tissue) in rats fed with a blackberry anthocyaninenriched diet [54]. In this study the bladder contained the highest levels of anthocyanins, followed by the prostate.…”

Cyanidin-3-glucoside derived from black soybeans ameliorate type 2 diabetes through the induction of differentiation of preadipocytes into smaller and insulin-sensitive adipocytes

“…2D, E), providing evidence that Cy3G-containing black soybean seed coat induces preadipocyte differentiation into smaller adipocytes. In a study investigating the distribution of anthocyanins in various organs in rats fed with a Cy3G-containing blackberry, Cy3G was detected in adipose tissue [39], which clearly demonstrates that Cy3G can reach adipocytes through the circulating system.…”

Tissue bioavailability of anthocyanins from whole tart cherry in healthy rats

“…We previously showed in humans that intake of individually quick frozen (IQF) whole tart cherries yielded plasma and urine anthocyanins and their related metabolites (Uhley et al, 2009). Because anthocyanins have perceived low tissue bioavailability, limited studies have measured tissue levels following intake of anthocyanin-rich foods (Felgines et al, 2009; Passamonti, Vrhovsek, Mattivi, & Vanzo, 2005;Talavéra et al, 2005). Furthermore, tissue bioavailability of tart cherry anthocyanins is unknown.…”