Tissue Culture in the Improvement of Salt Tolerance in Plants

In Vitro Selection of Salt Tolerant Calli Lines and Regeneration of Salt Tolerant Plantlets in Mung Bean (Vigna radiata L. Wilczek)

“…However the structural complexity of the whole plant makes it difficult to separate systemic from cellular salinity tolerance mechanism (Hawkins & Lips, 1997). The importance of plant tissue culture in the improvement of salt tolerance in plants has been pointed long back (Dix, 1993;Hasegawa, et al, 1994;Nabors et al, 1980;Tal 1994). In recent years tissue culture techniques are being used as a useful tool to elucidate the mechanism involved in salt tolerance by using in vitro selected salt tolerant cell lines (Davenport, et al, 2003;Gu, et al 2004;Lutts et al, 2004 ;Naik & Harinath, 1998;Rao & Patil.…”

Cellular Mechanisms of Salt Tolerance in Plant Cells

“…Recent reviews (Tall 990; Dracup 1991; Hasegawa et al 1994) provide extensive summaries of research that has been directed toward the goal of utilizing somaclonal variation (variation among plants regenerated in vitro from tissue cultured cells) and cell selection to obtain salt-tolerant plants. These reviews provide a concise analysis of the research and also highlight the rationale for exploiting this approach to obtain salt-tolerant plants.…”

“…With both flax (McHughen 1987) and sorghum (Bhaskaran et al 1986;Waskom et al 1990) plants regenerated from selected cells exhibited greater salt tolerance than the original explant source, and this tolerance appeared to be inherited in a stable manner. Extensive research on cells selected in culture has led to a better understanding of the roles of genetic determination versus elastic physi010gical adjustment in salt tolerance (Hasegawa et al 1994). This has facilitated the development of more effective strategies to obtain salttolerant plants via cell selection and will hopefully lead to greater success with this approach.…”

Cross Tolerance Mechanisms of Osmotic and Ionic Stress Adapted Cell Lines of Rice Towards Salinity

“…For success of biological strategy, the proposed logical approach is to dissect the complexity of the stress into components parts and complexity of tolerance mechanism into determinants of tolerance (Flowers and Yeo, 1995). Tissue culture technology simplifies the complexity of salt stress by growing plant/cell in in vitro to minimize interaction with environment and by growing/selecting cell lines/calli lines, which bypasses differentiation and structural integrity of plants (Hasegawa et al, 1994). The only possible option to dissect the stress tolerance mechanism into component part is to distinguish toxicity component of stress from osmotic and ionic component of salt stress i.e., tolerance to osmotic, toxic and ionic imbalance by exploitation of phenomenon of cross tolerance (Munns, 1993;Shah et al, 1993).…”