Tissue culture coupled with a gas exchange system offers new perspectives on phenotyping the developmental biology of Solanum lycopersicum L. cv. ‘MicroTom’

Abstract

Solanum lycopersicum L. cv. ‘Microtom’ (MicroTom) is a model organism with a relatively rapid life cycle, and wide library of genetic mutants available to study different aspects of plant development. Despite its small stature, conventional MicroTom research often requires expensive growth cabinets and/or expansive greenhouse space, limiting the number of experimental and control replications needed for experiments, and can render plants susceptible to pests and disease. Thus, alternative experimental approaches must be devised to reduce the footprint of experimental units and limit the occurrence problematic confounding variables. Here, tissue culture is presented as a powerful option for MicroTom research that can quell the complications associated with conventional MicroTom research methods. A previously established, non-invasive, analytical tissue culture system is used to compare in vitro and conventionally produced MicroTom by assessing photosynthesis, respiration, diurnal carbon gain, and fruit pigments. To our knowledge, this is the first publication that measures in vitro MicroTom fruit pigments and compares diurnal photosynthetic/respiration responses to abiotic factors between in vitro and ex vitro MicroTom. Comparable trends would validate tissue culture as a new benchmark method in MicroTom research, as it is like Arabidopsis, allowing replicable, statistically valid, high throughput genotyping and selective phenotyping experiments. Combining the model plant MicroTom with advanced tissue culture methods makes it possible to study bonsai-style MicroTom responses to light, temperature, and atmospheric stimuli in the absence of confounding abiotic stress factors that would otherwise be unachievable using conventional methods.

Machine learning-mediated Passiflora caerulea callogenesis optimization

“…The micropropagation procedure, as vegetative reproduction in in vitro cultures, is an excellent way to obtain clones (i.e., plants genetically identical to the parent plants) [61][62][63], in vitro developmental biology study [64][65][66], and genetic improvement through genetic engineering approaches [67]. One of the most effective in vitro culture methods is callogenesis, where callus is used to obtain de novo shoots and/or secondary metabolites [68,69].…”

Effect of Explant Source on Phenotypic Changes of In Vitro Grown Cannabis Plantlets over Multiple Subcultures

“…Micropropagation is an application of plant tissue culture that represents an alternative and promising approach for large-scale cannabis propagation of genetically uniform plants with high phytosanitary quality that can be produced in confined areas [ 6 , 7 , 9 ]. In addition to mass propagation, plant tissue culture is fundamental to biotechnology, allowing researchers to study developmental biology [ 10 , 11 ], secondary metabolite production [ 12 ], bioenergy production [ 13 ], cryopreservation [ 14 ], and transgenic- and genome-editing-based methods [ 15 ]. However, culture decline is a problem commonly encountered in tissue culture, which refers to the gradual loss of vigour and capacity for growth in the cultured cells and/or tissues [ 16 ].…”

Comparative analysis of different artificial neural networks for predicting and optimizing in vitro seed germination and sterilization of petunia

“…In this regard, the development of plant tissue culture techniques can be considered as one of the desirable methodologies for the micropropagation and biotechnological exploitation of many plant species with added-value properties [ 6 , 7 ]. Besides this, in vitro culture techniques can be utilized for the rapid production of true-to-type plants [ 7 – 15 ], developmental biology [ 16 – 21 ], secondary metabolite production [ 22 – 25 ], and bioenergy production [ 26 ]. Several studies have previously attempted on applying in vitro culture techniques to propagate the different cultivars of petunia [ 2 , 4 , 5 ].…”