Tissue Culture—A Sustainable Approach to Explore Plant Stresses

Abstract

Plants are constantly faced with biotic or abiotic stress, which affects their growth and development. Yield reduction due to biotic and abiotic stresses on economically important crop species causes substantial economic loss at a global level. Breeding for stress tolerance to create elite and superior genotypes has been a common practice for many decades, and plant tissue culture can be an efficient and cost-effective method. Tissue culture is a valuable tool to develop stress tolerance, screen stress tolerance, and elucidate physiological and biochemical changes during stress. In vitro selection carried out under controlled environment conditions in confined spaces is highly effective and cheaper to maintain. This review emphasizes the relevance of plant tissue culture for screening major abiotic stresses, drought, and salinity, and the development of disease resistance. Further emphasis is given to screening metal hyperaccumulators and transgenic technological applications for stress tolerance.

Teaching and Learning Biotechnology at University of Rwanda - College of Science and Technology: The Assessment of Teaching Practices and Learning Styles for Biotechnology Concepts Understanding

“…International Service for Agri-biotech Acquisition Applications reported that the potential achievements in agriculture depend on biotechnology (Utomo et al, 2017). Wijerathna-Yapa and Hiti-Bandaralage (2023) demonstrated that tissue culture and recombinant DNA are technologies utilized to improve crops' agronomic traits, resistance to unfavourable environmental factors and enhanced nutritional values. However, risks and ethical problems associated with the practices of biotechnology in agriculture have led to the limitation of its usage (Montgomery, 2015).…”

Metabolome profiling of cacao (Theobroma cacao L.) callus under drought stress conditions induced by polyethylene glycol (PEG) as osmoticant

“…17,18 The utilization of tissue culture techniques for quantifying stress tolerance of various crops has been increasing rapidly. [19][20][21] Polyethylene glycol (PEG), a non-toxic water-soluble polymer which is neither metabolized nor absorbed by cells, is available in a wide range of molecular weights (e.g., PEG-4000, PEG-4500, PEG-6000, PEG-8000, and PEG-10000). 22 It induces water deficit in in vitro tissue cultures in a manner similar to that observed in vivo in cells of intact plants subject to drought conditions.…”

“…Morpho‐physiological responses of young cacao hybrids to different water deficit stress conditions showed significant differences under control conditions 17,18 . The utilization of tissue culture techniques for quantifying stress tolerance of various crops has been increasing rapidly 19–21 . Polyethylene glycol (PEG), a non‐toxic water‐soluble polymer which is neither metabolized nor absorbed by cells, is available in a wide range of molecular weights (e.g., PEG‐4000, PEG‐4500, PEG‐6000, PEG‐8000, and PEG‐10000) 22 .…”

The Optimization of Medium Conditions and Auxins in the Induction of Adventitious Roots of Pokeweed (Phytolacca americana L.) and Their Phytochemical Constituents

“…Plant tissue culture is generally used for plant propagation under aseptic conditions. This technique is now widely applied in different fields because it can be used under time and space constraints [ 16 ]. Plant tissue culture is employed for large-scale production of new whole plants or specific plant parts.…”