Tissue alkaline phosphatase is involved in lipid metabolism and gene expression and secretion of adipokines in adipocytes

Hérnandez-Mosqueira, Claudio; Velez-delValle, Cristina; Kuri‐Harcuch, Walid

doi:10.1016/j.bbagen.2015.09.014

Cited by 34 publications

(28 citation statements)

References 54 publications

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“…Another possible explanation might be the influence of sex driven hormonal synthesis on adiposity. 16 In addition, it was earlier shown in vitro that tissue alkaline phosphatase knockdown increased the mRNA levels of adipokines including adiponectin but decreased leptin levels, 17 indicating an effect of alkaline phosphatases on lipid metabolism and adipokine secretion. It therefore, might be possible that sex and AKP3 deficiency may have synergistic effects.…”

Section: Discussionmentioning

confidence: 98%

Adiposity and metabolic health in mice deficient in intestinal alkaline phosphatase

et al. 2018

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Intestinal alkaline phosphatase 3 (AKP3) is an enzyme that was reported to play a role in lipid metabolism and to prevent high fat diet-induced metabolic syndrome in mice. To investigate a potential functional role of AKP3 in diet-induced adiposity and metabolic health, we have kept male and female wild-type or AKP3 deficient mice on a high fat diet for 15 weeks to induce obesity and compared those with mice kept on standard fat diet. Body weight as well as adipose tissue mass were statistically significantly higher upon high fat diet feeding for mice of both genders and genotypes. Female mice of either genotype kept on high fat diet gained less weight, resulting in smaller adipose tissue depots with smaller adipocytes. However, AKP3 deficiency had no significant effect on body weight gain or adipose tissue mass and did not affect adipocyte size or density. Gene expression analysis revealed no effect of the genotype on inflammatory parameters in adipose tissue, except for tumor necrosis factor alpha, which was higher in mesenteric adipose tissue of female obese mice. Plasma glucose and insulin levels were also not affected in obese AKP3 deficient mice. Overall, our data do not support a functional role of AKP3 in adipose tissue development, or insulin sensitivity.

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Section: Discussionmentioning

confidence: 98%

Adiposity and metabolic health in mice deficient in intestinal alkaline phosphatase

et al. 2018

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“…), the murine 3T3‐F442A pre‐adipocyte cell line (Hernández‐Mosqueira et al . ), adipocyte precursor cells in human bone marrow (Bianco et al . ), rabbit pre‐adipocytes (Lecoeur & Ouhayoun ), murine bone marrow pre‐adipocytes (Kodama et al .…”

Section: Discussionmentioning

confidence: 99%

“…) and 3T3‐F422A (Hernández‐Mosqueira et al . ). TNSALP is also expressed in HepG2 cells (Nowrouzi & Yazdanparast ).…”

mentioning

confidence: 97%

Specific knock‐down of tissue non‐specific alkaline phosphatase mRNA levels inhibits intracellular lipid accumulation in 3T3‐L1 and HepG2 cells

Chirambo

Niekerk

Crowther

2017

Int J Experimental Path

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The use of non-specific inhibitors of tissue non-specific alkaline phosphatase (TNSALP) in pre-adipocytes blocks intracellular lipid accumulation. TNSALP is also expressed in hepatocytes, which are known to accumulate lipid in a similar manner to pre-adipocytes. The purpose of this study was to use specific silencing of TNSALP mRNA, using short interfering (si) RNA, to investigate the role of TNSALP in intracellular lipid accumulation in 3T3-L1 and HepG2 cells. Cellular activity of TNSALP was measured using an automated colorimetric assay, and intracellular lipid accumulation was determined using the lipid-specific dye, Oil Red O. Cells were transfected with siRNA directed against TNSALP mRNA, and expression of the TNSALP gene was determined at selected time points postinduction of lipid droplet formation. Expression of the TNSALP gene was inhibited by a maximum of 88 ± 1.9% (P < 0.005 vs. control) 11 days after initiation of lipid droplet formation in the 3T3-L1 cells and 80 ± 8.9% (P < 0.05 vs. control) after 4 days in the HepG2 cells. This led to significant inhibition of both TNSALP activity and intracellular lipid accumulation in both cell lines. These data demonstrates that TNSALP plays an important role in the control of lipid droplet formation in both pre-adipocyte and hepatocyte cell lines.

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“…Nevertheless, 3T3-F442A cells have also been used to study the effects of compounds during the differentiation process. Moreover, gene silencing through siRNA has been carried out to study the role of alkaline phosphatase in lipid metabolism and gene expression as well as the secretion of adipokines [ 52 ]. Additionally, this cell line has been used to study the effects of some drugs on adipocyte differentiation [ 53 ].…”

Section: Animal Cell Modelsmentioning

confidence: 99%

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Ruiz‐Ojeda

Rupérez

Gómez-Llorente

et al. 2016

IJMS

293

221

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Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are complex processes, which can be investigated thanks to the development of diverse in vitro cell models and molecular biology techniques that allow for a better understanding of adipogenesis and adipocyte dysfunction associated with obesity. The aim of the present work was to update the different animal and human cell culture models available for studying the in vitro adipogenic differentiation process related to obesity and its co-morbidities. The main characteristics, new protocols, and applications of the cell models used to study the adipogenesis in the last five years have been extensively revised. Moreover, we depict co-cultures and three-dimensional cultures, given their utility to understand the connections between adipocytes and their surrounding cells in adipose tissue.

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Tissue alkaline phosphatase is involved in lipid metabolism and gene expression and secretion of adipokines in adipocytes

Cited by 34 publications

References 54 publications

Adiposity and metabolic health in mice deficient in intestinal alkaline phosphatase

Adiposity and metabolic health in mice deficient in intestinal alkaline phosphatase

Specific knock‐down of tissue non‐specific alkaline phosphatase mRNA levels inhibits intracellular lipid accumulation in 3T3‐L1 and HepG2 cells

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

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