Adiposity and metabolic health in mice deficient in intestinal alkaline phosphatase

Abstract

Intestinal alkaline phosphatase 3 (AKP3) is an enzyme that was reported to play a role in lipid metabolism and to prevent high fat diet-induced metabolic syndrome in mice. To investigate a potential functional role of AKP3 in diet-induced adiposity and metabolic health, we have kept male and female wild-type or AKP3 deficient mice on a high fat diet for 15 weeks to induce obesity and compared those with mice kept on standard fat diet. Body weight as well as adipose tissue mass were statistically significantly higher upon high fat diet feeding for mice of both genders and genotypes. Female mice of either genotype kept on high fat diet gained less weight, resulting in smaller adipose tissue depots with smaller adipocytes. However, AKP3 deficiency had no significant effect on body weight gain or adipose tissue mass and did not affect adipocyte size or density. Gene expression analysis revealed no effect of the genotype on inflammatory parameters in adipose tissue, except for tumor necrosis factor alpha, which was higher in mesenteric adipose tissue of female obese mice. Plasma glucose and insulin levels were also not affected in obese AKP3 deficient mice. Overall, our data do not support a functional role of AKP3 in adipose tissue development, or insulin sensitivity.

Alkaline Phosphatase Relieves Colitis in Obese Mice Subjected to Forced Exercise via Its Anti-Inflammatory and Intestinal Microbiota-Shaping Properties

“…Our observations regarding the beneficial effects of IAP are supported by earlier reports that a reduced expression of IAP was found in IBD, metabolic syndrome, cystic fibrosis, necrotizing enterocolitis, and diabetes [19,28,31,32], and IAP deficiency has been linked with obesity and IBD [10,28,33,34]. Moreover, this enzyme has been included as essential in the mechanism of normalizing of the homeostasis of the intestinal microbiota [32].…”

The Combination of Intestinal Alkaline Phosphatase Treatment with Moderate Physical Activity Alleviates the Severity of Experimental Colitis in Obese Mice via Modulation of Gut Microbiota, Attenuation of Proinflammatory Cytokines, Oxidative Stress Biomarkers and DNA Oxidative Damage in Colonic Mucosa

“…A decreased expression of IAP is found in disorders such as IBD, metabolic syndrome, cystic fibrosis, necrotizing enterocolitis and diabetes [ 27 , 28 , 29 , 30 , 31 , 32 ]. Moreover, IAP deficiency has been linked with obesity and IBD [ 30 , 33 , 34 ]. IAP has also been considered in the mechanism of normalization of intestinal microbiota, thereby regulating the intestinal homeostasis.…”

Albumin-to-Alkaline Phosphatase Ratio as a Prognostic Biomarker for Spinal Fusion in Lumbar Degenerative Diseases Patients Undergoing Lumbar Spinal Fusion

“…ALP catalyzes the hydrolysis of phosphate esters and is responsible for transferring phosphate groups, which are mainly produced in the liver and bone. ALP activity could reflect the metabolism and immunity of the body and be used as an immunometric [ 31 , 32 ]. ALP activity is increased in various hepatobiliary diseases, rickets, osteogenesis imperfection, osteomalacia, etc.…”