Tipificación molecular de cepas de Mycobacterium avium subespecie paratuberculosis de diferentes huéspedes y regiones

Sevilla, Iker A.; Singh, Shoor Vir; Garrido, Joseba M.; Aduriz, G.; Rodríguez, Sabrina; Geijó, Mariví; Whittington, Richard J.; Saunders, Vanessa F.; Whitlock, Robert H.; Juste, Ramón A.

doi:10.20506/rst.24.3.1634

Cited by 97 publications

(66 citation statements)

References 8 publications

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“…paratuberculosis infection. Results obtained in this study support the findings of Sevilla et al (2005) who reported that primers targeted against IS900 and IS1311 can amplify DNA of M. avium subsp. paratuberculosis.…”

Section: Bpsupporting

confidence: 91%

Detection of Mycobacterium avium subsp. paratuberculosis in tissue samples of cattle and buffaloes

Khan¹,

Chaudhry

Ali³

et al. 2009

Trop Anim Health Prod

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Tissue samples were collected at random from cattle (Bos taurus) and buffalo (Bubalus bubalis) from an abattoir of the district of Lahore and were analyzed for the presence of Mycobacterium avium subsp. paratuberculosis and Mycobacterium bovis through acid-fast staining and polymerase chain reaction (PCR). Body condition of animals and diarrhea were recorded. Most of the animals were emaciated. Diarrhea was noticed in 15.6% of buffaloes and 19.2% of cattle. Intestinal pathology was observed in 29% of buffaloes and 32.8% of cattle. Number of mesenteric lymph node (MLN) showing gross lesions was a bit higher (35.6%) in cattle than buffalo (31.2%). Acid-fast staining of tissue scraping smears revealed the presence of acid-fast bacilli (AFB) in 17.4% intestinal and 16.4% MLN tissue samples in buffalo, while in cattle 19.2% intestinal and 17.8% MLN were found positive for AFB. In buffaloes, PCR confirmed 12.8% intestinal and 12.4% MLN positive samples for M. avium subsp. paratuberculosis. However, in cattle, PCR analysis demonstrated 14.2% positive results for M. avium subsp. paratuberculosis in both MLN and intestinal tissue samples. PCR also confirmed M. bovis in 5.8% of cattle and 5% of buffalo MLN and intestinal tissues. PCR positive tissue samples for M. avium subsp. paratuberculosis were from those animals which were emaciated, having diarrhea, and severe gross lesions. AFB were also detected in tissue scraping smears of these animals. It is concluded that infection by various mycobacterium species can be differentiated by PCR, which is not possible by acid-fast staining technique.

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Section: Bpsupporting

confidence: 91%

Detection of Mycobacterium avium subsp. paratuberculosis in tissue samples of cattle and buffaloes

Khan¹,

Chaudhry

Ali³

et al. 2009

Trop Anim Health Prod

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show abstract

“…While historically, cattle were infected mainly with Type II strains (Collins et al, 1990;Sevilla et al, 2005;Motiwala et al, 2006b), results from the present study indicate that Type I strains are more frequent in New Zealand beef cattle than Type II. The most common subtype (C) was mainly isolated from sheep and beef cattle located in the NI.…”

Section: Discussioncontrasting

confidence: 41%

“…This could explain the low cPtb incidence observed in New Zealand beef cattle (Verdugo et al, 2011). The previously suggested host specialization of MAP strains between cattle and sheep populations (Collins et al, 1990;Whittington et al, 2001;Sevilla et al, 2005) could reflect a lack of interaction between these species, rather than a true adaptation to specific host populations (Sevilla et al, 2005;Motiwala et al, 2006b).…”

Section: Discussionmentioning

confidence: 95%

“…Previous studies using samples from New Zealand, Australia and Spain showed that Type II strains were commonly isolated from cattle, deer and goats (Collins et al, 1990;Sevilla et al, 2005). Conversely, Type I strains were mainly isolated from sheep (Collins et al, 1990;Sevilla et al, 2005;Motiwala et al, 2006b). However, opposing outcomes in strain segregation have been observed recently in a multi-host molecular study of MAP isolates across Europe (Stevenson et al, 2009).…”

Section: Introductionmentioning

confidence: 98%

“…In particular, analyses using RFLP and PFGE techniques have identified two major strain groups (or types), named Type I (ovine or type S) and Type II (bovine or type C) (Collins et al, 1990;Bauerfeind et al, 1996;Stevenson et al, 2002). Previous studies using samples from New Zealand, Australia and Spain showed that Type II strains were commonly isolated from cattle, deer and goats (Collins et al, 1990;Sevilla et al, 2005). Conversely, Type I strains were mainly isolated from sheep (Collins et al, 1990;Sevilla et al, 2005;Motiwala et al, 2006b).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis isolated from sheep, cattle and deer on New Zealand pastoral farms

Verdugo

Pleydell

Price-Carter

et al. 2014

Preventive Veterinary Medicine

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Multiplex qPCR for differentiation of Mycobacterium avium subspecies paratuberculosis in active and passive infection of goats

Pachoori

Kumaresan

Sachan

et al. 2022

Appl Microbiol Biotechnol

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Tipificación molecular de cepas de Mycobacterium avium subespecie paratuberculosis de diferentes huéspedes y regiones

Cited by 97 publications

References 8 publications

Detection of Mycobacterium avium subsp. paratuberculosis in tissue samples of cattle and buffaloes

Detection of Mycobacterium avium subsp. paratuberculosis in tissue samples of cattle and buffaloes

Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis isolated from sheep, cattle and deer on New Zealand pastoral farms

Multiplex qPCR for differentiation of Mycobacterium avium subspecies paratuberculosis in active and passive infection of goats

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