Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis isolated from sheep, cattle and deer on New Zealand pastoral farms

Verdugo, Cristóbal; Pleydell, Eve; Price-Carter, Marian; Prattley, D. J.; Collins, Desmond M.; Lisle, Geoffrey de; Vogue, Hinrich; Wilson, Peter R.; Heuer, C

doi:10.1016/j.prevetmed.2014.09.009

Cited by 28 publications

(31 citation statements)

References 49 publications

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“…Mycobacterium avium is a nontuberculous mycobacteria which can be found widely in the environment in water, soil, dust [ 39 ] and in animals like cow, deer, sheep [ 40 , 41 ] and humans [ 39 ]. Like Mycobacterium tuberculosis , Mycobacterium avium can invade host macrophages and avoid being killed by preventing phagosomal maturation [ 42 ], causing granuloma and chronic infection as a result.…”

Section: Discussionmentioning

confidence: 99%

Differences in pathogenicity of three animal isolates of Mycobacterium species in a mouse model

Dong

Sreevatsan

et al. 2017

PLoS ONE

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Animal mycobacterioses are among the most important zoonoses worldwide. These are generally caused by either Mycobacterium tuberculosis (MTB), M. bovis (MBO) or M. avium (MAV). To test the hypothesis that different species of pathogenic mycobacteria isolated from varied anatomic locations or animal species differ in virulence and pathogenicity, we performed experiments with three mycobacteria strains (NTSE-3(MTB), NTSE-4(MBO) and NTSE-5 (MAV)) obtained from animal species. Spoligotyping analysis was used to confirm both MTB and MBO strains while the MAV strain was confirmed by 16s rDNA sequencing. BALB/c mice were intranasally infected with the three strains at low and high CFU doses to evaluate variations in pathogenicity. Clinical and pathological parameters were assessed. Infected mice were euthanized at 80 days post-inoculation (dpi). Measures of lung and body weights indicated that the MBO infected group had higher mortality, more weight loss, higher bacterial burden and more severe lesions in lungs than the other two groups. Cytokine profiles showed higher levels of TNF-α for MBO versus MTB, while MAV had the highest amounts of IFN-β in vitro and in vivo. In vitro levels of other cytokines such as IL-1β, IL-10, IL-12, IL-17, and IFN-β showed that Th1 cells had the strongest response in MBO infected mice and that Th2 cells were inhibited. We found that the level of virulence among the three isolates decreased in the following order MBO>MTB>MAV.

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Section: Discussionmentioning

confidence: 99%

Differences in pathogenicity of three animal isolates of Mycobacterium species in a mouse model

Dong

Sreevatsan

et al. 2017

PLoS ONE

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“…Due to the subclinical infections and lack of data on prevalence, morbidity and mortality caused by MAP, JD has not received the priority in developing countries (Verdugo, 2013). In Australia, average mortality rate of ovine JD (OJD) was estimated in 12 farms as 6.2% (range 2.1 to 17.5%) in 2002 and 7.8% (range 1.8 to 14.6%) in 2003 (Sulficar et al, 2009).…”

Section: Morbidity and Mortalitymentioning

confidence: 99%

“…There is a genetic predisposition to develop CD, recently 3 independent studies suggested that mutation in a gene on chromosome 16, known as NOD2/CARD 15 are associated with CD (Verdugo, 2013: Verma,2013.…”

Section: Map Infection In Human (Crohn's Disease)mentioning

confidence: 99%

“…But, MAP has the attention for the causative agent of CD due to the clinical signs and pathological similarity of JD with CD initially and later frequently isolation of MAP from tissues of CD patients (Verdugo, 2013). Early studies did not detect MAP in tissues from patients with CD by conventional staining and culture techniques (Naser et al, 2004).…”

Section: Map Infection In Human (Crohn's Disease)mentioning

confidence: 99%

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Mycobacterium avium subspecies paratuberculosis: An Emerging Bacterial Disease of Global Public Health Significance

Rahman

2016

Microbes & Health

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Mycobacterium avium subspecies paratuberculosis (MAP) is the etiological agent of chronic enteric disease of ruminant known as paratuberculosis (Johne's disease). The disease causes considerable economic losses worldwide due to reduced milk production and eventually, diarrhoea, weight loss and death. Johne's disease (JD) has some pathological similarities with Crohn's disease (CD)in humans, and the role of MAP in the causation of CD has been under investigation for last 100 years. Animals infected with JD shed viable MAP in the blood, and tissues.Consequently, transmission to humans may occur via consumption of animal derived foods. In developing countries, limited information is available on the occurrence of MAP infection in animals and humans. MAP infection has been established in animals and humans may get the MAP exposure through food chain or contaminated environment. Presently, MAP is of great public health significance because it is speculated to be involved in Crohn's disease in humans. The present review summarizes the information primarily on the nature of MAP in animals and humans, economic losses and morbidity and mortality due to JD and CD at global level. Current concept on the possible relationship between MAP and Crohn's disease has also been reviewed.

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“…analysis. To date 139 genotypes have been deposited in the European VNTR database of Mycobacterium avium complex in France (available at: mac-inmv.tours.inra.fr/) (10)(11)(12)(13)(14). MIRU-VNTR genotyping is a PCR-based system currently applied on numerous pathogenic bacteria (15)(16)(17)(18) in which the copy number of specific genomic unit repeats, known as Tandem Repeats, in the genome of a given bacterium is determined.…”

mentioning

confidence: 99%

Genomic Characterization of the Vaccinal Strain of Mycobacterium Avium Subspecies Paratuberculosis (MAP) 316F by MIRU-VNTR

2015

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Background and Objective: Paratuberculosis is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). this study aimed to characterize the genome of the MAP 316F strain. Methods: The MAP 316F strain was subjected to the PCR-F57 and PCR-IS900 experiments in order to ensure its identity as MAP. This was followed by application of the Thibault genotyping system consisting of eight loci including 292, x3, 25, 47, 3, 7, 10 and 32. Required genomic material for all experiments was prepared using the simple method of boiling. Gel electrophoresis findings related to the typing PCRs were backed by sequencing of amplification products.

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Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis isolated from sheep, cattle and deer on New Zealand pastoral farms

Cited by 28 publications

References 49 publications

Differences in pathogenicity of three animal isolates of Mycobacterium species in a mouse model

Differences in pathogenicity of three animal isolates of Mycobacterium species in a mouse model

Mycobacterium avium subspecies paratuberculosis: An Emerging Bacterial Disease of Global Public Health Significance

Genomic Characterization of the Vaccinal Strain of Mycobacterium Avium Subspecies Paratuberculosis (MAP) 316F by MIRU-VNTR

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