Human tuberculosis remains a huge global public health problem with an estimated 1/3rd of the population being infected. Defensins are antibacterial cationic peptides produced by a number of cell types, most notably neutrophil granulocytes and epithelial cells. All three defensin types (α-, β-, and θ-defensins) have antibacterial activities, mainly through bacterial membrane permeabilization. Defensins are effective against Gram-negative and Gram-positive bacteria including mycobacteria and are active both intra- and extracellularly. Mycobacterial resistance has never been demonstrated although the mprF gene encoding resistance in Staphylococcus aureus is present in the Mycobacterium tuberculosis genome. In addition to their antibacterial effect, defensins are chemoattractants for macrophages and neutrophils. There are many cases for their use for therapy or prophylaxis in tuberculosis as well. In conclusion, we propose that there is considerable scope and potential for exploring their use as therapeutic/prophylactic agents and more comprehensive survey of defensins from different species and their bioactivity is timely.

Animal mycobacterioses are among the most important zoonoses worldwide. These are generally caused by either Mycobacterium tuberculosis (MTB), M. bovis (MBO) or M. avium (MAV). To test the hypothesis that different species of pathogenic mycobacteria isolated from varied anatomic locations or animal species differ in virulence and pathogenicity, we performed experiments with three mycobacteria strains (NTSE-3(MTB), NTSE-4(MBO) and NTSE-5 (MAV)) obtained from animal species. Spoligotyping analysis was used to confirm both MTB and MBO strains while the MAV strain was confirmed by 16s rDNA sequencing. BALB/c mice were intranasally infected with the three strains at low and high CFU doses to evaluate variations in pathogenicity. Clinical and pathological parameters were assessed. Infected mice were euthanized at 80 days post-inoculation (dpi). Measures of lung and body weights indicated that the MBO infected group had higher mortality, more weight loss, higher bacterial burden and more severe lesions in lungs than the other two groups. Cytokine profiles showed higher levels of TNF-α for MBO versus MTB, while MAV had the highest amounts of IFN-β in vitro and in vivo. In vitro levels of other cytokines such as IL-1β, IL-10, IL-12, IL-17, and IFN-β showed that Th1 cells had the strongest response in MBO infected mice and that Th2 cells were inhibited. We found that the level of virulence among the three isolates decreased in the following order MBO>MTB>MAV.

Pumped-storage power stations play a regulatory role in the power grid through frequent transition processes. The pressure pulsation in the draft tube of the pump-turbine under transient processes is important for safe operation, which is more intense than that in the steady-state condition. However, there is no effective method to obtain the exact pressure in the draft tube in the transient flow field. In this paper, the pressure in the draft tube of a pump-turbine under steady-state and transient conditions are studied by means of CFD. The reliability of the simulation method is verified by comparing the real pressure pulsation data with the test results. Due to the distribution of the pressure pulsation in the draft tube being complex and uneven, the location of the pressure monitoring points directly affects the accurate judgement of cavitation. Eight monitoring surfaces were set in the straight cone of the draft tube and nine monitoring points were set on each monitoring surface to analyze the pressure differences on the wall and inside the center of the draft tube. The relationships between the pressure pulsation value inside the center of the draft tube and on the wall are studied. The “critical” wall pressure pulsation value when cavitation occurs is obtained. This study provides references for judging cavitation occurrences by using the wall pressure pulsation value in practical engineering.

Axial hydraulic thrust is an important factor that affects safety and stability of pump turbine operation. Research and analysis of axial hydraulic thrust is of a great significance for guiding the safe and stable operation of a pumped storage power station. Since the runner shape of the pump turbine is flat and its radial dimension is large, an increase of leakage can happen easily. In order to reduce the leakage and improve the efficiency of the unit, a labyrinth ring seal is usually used in the upper crown and lower ring of the runner because the inner clearance of the seal has a great influence on the axial thrust. In order to study the influence of the change of labyrinth seal position on axial hydraulic thrust, a fluid domain model with a pressure balance pipe, upper crown clearance, and lower ring clearance is established for a pump turbine of a power station. The distribution position of labyrinth ring in the upper crown clearance is changed. The CFD numerical simulations are carried out under both 100% working load and 75% working load of turbine conditions, considering the flow in clearance areas. The research results of this paper have found that the axial hydraulic thrust of the 100% load condition is consistent with the change of the gap position compared with the 75% load condition. The amplitude of the change of the water thrust under the 100% load condition is greater. As the sealing position of the labyrinth ring in the upper crown gap moves away from the central axis, the resultant vertical and upward water thrust increases, and the operating efficiency of the unit first increases and then decreases. As the position of the labyrinth ring seal in the upper ring clearance moves away from the central axis, the resultant vertical and upward water thrust increases, and the operating efficiency of the unit first increases and then decreases. Defining the radial dimension ratio δ between the front clearance area and the total area of labyrinth ring, the closer δ is to 0.5, the unit efficiency is higher; the smaller that δ is, then the high pressure area in the upper crown clearance is smaller, and the hydraulic thrust force increases vertically. Considering a variety of factors, the clearance seal position has the optimal value. In the practical application of the project, the condition of excessive upward hydraulic thrust leading to the lifting of the unit can be avoided, and the phenomenon of excessive downward hydraulic thrust leading to the excessive load-bearing of the frame is evitable.

As the corroded metal grounding usually has a high resistance, the paper presents a new kind of effective graphite grounding conductor to solve the related problems during the reform or construction process. By discussing the ratio of raw material, the structure designs, the principle of reducing resistance and the installation methods in detail, it proves the effectiveness of this grounding conductor. The formula made up with graphite, high-grade cement, small stones and sands, the graphite grounding conductor can show the characteristics of stable performance, excellent corrosion resistance, non-toxic and pollution-free. In addition, the structure of corrugation or cavity can effectively reduce the ground resistance, and it is quite significant for the safe and reliable operation of the power grid.