Tinea Unguium: Diagnosis and Treatment in Practice

Asz-Sigall, Daniel; Tosti, Antonella; Arenas, Roberto

doi:10.1007/s11046-016-0078-4

Cited by 36 publications

(31 citation statements)

References 21 publications

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“…The most common subtype of onychomycosis is DLSO, which is characterized by fungal invasion of the nail bed and underside of the nail plate beginning at the hyponychium 16,20,21) . In contrast, fungal invasion in our model developed on the surface of the nail plate, after which the dermatophyte invaded the deep nail plate.…”

Section: Discussionmentioning

confidence: 99%

Development of an Animal Model of Onychomycosis in Guinea Pigs

Hasegawa

Shibuya

2020

Medical Mycology Journal

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Onychomycosis is a common and intractable superficial mycosis that occurs worldwide. Treatment with both oral and topical drugs is recommended, but the objective evaluation procedure to determine the efficacy of and the appropriate delivery system for the drugs remains controversial. This may be attributed to the lack of a reliable animal model that not only mirrors the pathophysiology of human onychomycosis but is also feasible. Therefore, we attempted to establish an animal model of onychomycosis using immunosuppressed guinea pigs and elucidate the pathophysiology of human onychomycosis. In the present study, we applied Trichophyton mentagrophytes TIMM2789 to the hind limb nails of corticosteroid-treated guinea pigs. The nails were examined macroscopically and histopathologically at 0, 14, and 42 days after a 2-week exposure period to the fungus. A large portion of the experimentally infected nails showed discoloration, which is an important clinical sign, and most infections were confirmed histopathologically in the deep layer of the nail plate at all time points. The infection rates at 0, 14, and 42 days after exposure were 39%, 61%, and 78%, respectively. Thus, we established an animal model of onychomycosis with good reproducibility and that might be appropriate for extrapolation to the pathophysiology of the human disease.

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Section: Discussionmentioning

confidence: 99%

Development of an Animal Model of Onychomycosis in Guinea Pigs

Hasegawa

Shibuya

2020

Medical Mycology Journal

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“…Systemic antifungals are the most effective treatment but topical therapy is less effective. 20 5-Flucytosine is a antifungal drug and also a antimetabolite agent which acts by blocking nucleic asid synthesis. Flucytosine enters the fungal cell via cytosine permease; thus, flucytosine is metabolized to 5-fluorouracil within fungal organisms.…”

Section: Discussionmentioning

confidence: 99%

A Case of Onychomycosis Due to Aspergillus flavus in all Fingernails and Toenails of an Immunocompromised Patient and Healing with 5-Fluorouracil Chemotherapy

Kara¹,

Çöloğlu²

2018

Turkiye Klinikleri J Case Rep

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“…Considering the low efficiency of chemical antifungals against dermatophytes [ 8 , 9 ], their elimination using biological defence means would appear as an attractive alternative. However, the biological enemy will have to offer universal and safe elimination mechanisms, considering the physiological and etiological variability among individual dermatophytes.…”

Section: Introductionmentioning

confidence: 99%

The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses

Gabrielová

Mencl²,

Suchánek

et al. 2018

Mycopathologia

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Pythium oligandrum (Oomycota) is known for its strong mycoparasitism against more than 50 fungal and oomycete species. However, the ability of this oomycete to suppress and kill the causal agents of dermatophytoses is yet to be studied. We provide a complex study of the interactions between P. oligandrum and dermatophytes representing all species dominating in the developed countries. We assessed its biocidal potential by performing growth tests, on both solid and liquid cultivation media and by conducting a pilot clinical study. In addition, we studied the molecular background of mycoparasitism using expression profiles of genes responsible for the attack on the side of P. oligandrum and the stress response on the side of Microsporum canis. We showed that dermatophytes are efficiently suppressed or killed by P. oligandrum in the artificial conditions of cultivations media between 48 and 72 h after first contact. Significant intra- and interspecies variability was noted. Of the 69 patients included in the acute regimen study, symptoms were completely eliminated in 79% of the patients suffering from foot odour, hyperhidrosis disappeared in 67% of cases, clinical signs of dermatomycoses could no longer be observed in 83% of patients, and 15% of persons were relieved of symptoms of onychomycosis. Our investigations provide clear evidence that the oomycete is able to recognize and kill dermatophytes using recognition mechanisms that resemble those described in oomycetes attacking fungi infecting plants, albeit with some notable differences.Electronic supplementary materialThe online version of this article (10.1007/s11046-018-0277-2) contains supplementary material, which is available to authorized users.

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Tinea Unguium: Diagnosis and Treatment in Practice

Cited by 36 publications

References 21 publications

Development of an Animal Model of Onychomycosis in Guinea Pigs

Development of an Animal Model of Onychomycosis in Guinea Pigs

A Case of Onychomycosis Due to Aspergillus flavus in all Fingernails and Toenails of an Immunocompromised Patient and Healing with 5-Fluorouracil Chemotherapy

The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses

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