Timing of oviductal fluid collection, steroid concentrations, and sperm preservation method affect porcine in vitro fertilization efficiency

Ballester, L.; Romero-Aguirregomezcorta, Jon; Soriano‐Úbeda, Cristina; Matás, Carmen; Romar, Raquel; Coy, Pilar

doi:10.1016/j.fertnstert.2014.08.009

Cited by 31 publications

(36 citation statements)

References 42 publications

(46 reference statements)

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“…In rabbits during estrus, oviductal progesterone and estradiol were measured at concentrations more or less similar to those measured in the circulating serum (0.5 ng/mL of progesterone and 50-120 pg/mL of estradiol) [52]. By contrast, in pigs, cows, and mares, recent studies indicate that progesterone and estradiol are present in tubal fluid at much higher concentrations than those observed in the serum [39,53,54]. The fold-changes between OF and serum are particularly high in the oviducts ipsilateral to the ovulation side in cows and mares, in which progesterone and estradiol reach 10 to 50 times higher levels in the tubal fluid than in the circulating blood [39,54].…”

Section: Hormonesmentioning

confidence: 79%

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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The oviductal fluid is the first environment experienced by mammalian embryos at the very beginning of life. However, it has long been believed that the oviductal environment was not essential for proper embryonic development. Successful establishment of in vitro embryo production techniques (which completely bypass the oviduct) have reinforced this idea. Yet, it became evident that in vitro produced embryos differ markedly from their in vivo counterparts, and these differences are associated with lower pregnancy outcomes and more health issues after birth. Nowadays, researchers consider the oviduct as the most suitable microenvironment for early embryonic development and a substantial effort is made to understand its dynamic, species-specific functions. In this review, we touch on the origin and molecular components of the oviductal fluid in mammals, where recent progress has been made thanks to the wider use of mass spectrometry techniques. Some of the factors and processes known to regulate oviductal secretions, including the embryo itself, as well as ovulation, insemination, endogenous and exogenous hormones, and metabolic and heat stress, are summarized. Special emphasis is laid on farm animals because, owing to the availability of sample material and the economic importance of fertility in livestock husbandry, a large part of the work on this topic has been carried out in domestic animals used for dairy and/or meat production.

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Section: Hormonesmentioning

confidence: 79%

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Saint‐Dizier

Schoen

Chen

et al. 2019

IJMS

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“…The P4 concentration in FF, OF and CM was assessed by chemiluminiscent microparticle immunoassay (CMIA; Architect, Abbott), as described19.…”

Section: Methodsmentioning

confidence: 99%

“…The OF concentration was based on previous studies6364. However, the reasons to supplement with 2% CM were basically mechanical (because 50 COCs were taken in a volume of 20 μl) and to ensure that the P4 concentration was within the range of values that occur in the oviduct during fertilization19. The spermatozoa capacitation status after incubation at pH e 8.0 and the addition of OFCM was also evaluated.…”

Section: Experimental Designmentioning

confidence: 99%

Improving porcine in vitro fertilization output by simulating the oviductal environment

Soriano‐Úbeda

García–Vázquez

Romero-Aguirregomezcorta

et al. 2017

Sci Rep

Self Cite

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Differences between the in vitro and in vivo environment in which fertilization occurs seem to play a key role in the low efficiency of porcine in vitro fertilization (IVF). This work proposes an IVF system based on the in vivo oviductal periovulatory environment. The combined use of an IVF medium at the pH found in the oviduct in the periovulatory stage (pHe 8.0), a mixture of oviductal components (cumulus-oocyte complex secretions, follicular fluid and oviductal periovulatory fluid, OFCM) and a device that interposes a physical barrier between gametes (an inverted screw cap of a Falcon tube, S) was compared with the classical system at pHe 7.4, in a 4-well multidish (W) lacking oviduct biological components. The results showed that the new IVF system reduced polyspermy and increased the final efficiency by more than 48%. This higher efficiency seems to be a direct consequence of a reduced sperm motility and lower capacitating status and it could be related to the action of OFCM components over gametes and to the increase in the sperm intracellular pH (pHi) caused by the higher pHe used. In conclusion, a medium at pH 8.0 supplemented with OFCM reduces polyspermy and improves porcine IVF output.

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“…For many years, studies on in vitro fertilization (IVF) in pigs have been conducted as an important tool for reproduction (Cheng et al 1986, Mattioli et al 1989, Zhang et al 2012, Ballester et al 2014. However, the procedures currently used for in vitro maturation (IVM) and IVF for in vitro production (IVP) of porcine embryos frequently result in low rates of embryonic development (Grupen et al 1997, Coy & Romar 2002.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have been conducted in an attempt to decrease the rate of polyspermy in IVF of porcine oocytes (Oberlender et al 2013a, Ballester et al 2014. In vivo, the incidence of polyspermy is less than 5% (Funahashi et al 2000).…”

Section: Introductionmentioning

confidence: 99%

In vitro fertilization of porcine oocytes is affected by spermatic coincubation time

et al. 2016

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The aim was to study the effects of different gamete coincubation times on porcine in vitro fertilization (IVF), and to verify whether efficiency could be improved by reducing oocyte exposure time to spermatozoa during IVF. In groups of 50, a total of 508 immature cumulus-oocyte complexes (COCs) were matured in NCSU-37 medium. The COCs were cultured for 44 hours and then inseminated with in natura semen (2,000 spermatozoa/ oocyte). The sperm and oocytes were coincubated according to the following treatments (T): T1 = oocytes exposed to spermatozoa for one hour (173 oocytes), T2 = oocytes exposed to spermatozoa for two hours (170 oocytes), and T3 = oocytes exposed to spermatozoa for three hours (165 oocytes). After these coincubation periods, the oocytes were washed in fertilization medium (TALP medium) to remove spermatozoa not bound to the zona pellucida and cultured in another similar medium (containing no sperm). Eighteen to twenty hours after fertilization, the putative zygotes were stained in Hoechst-33342 to evaluate the IVF results. The penetration rate was higher (P<0.05) after two hours of coincubation time than it was for one or three hours. Furthermore, 68.60% of the ova coincubated with the spermatozoa for two hours were monospermic. The oocytes exposed to spermatozoa for one hour (T1) presented a higher (P<0.01) rate of polyspermy than those in T2 and T3. Fertilization performance (%) did not differ (P>0.05) between oocytes exposed to spermatozoa for one (T1) and three hours (T3). However, optimum (P=0.048) results were obtained after two hours of coincubation, when the rate of fertilization performance was 50.16±8.52%. The number of penetrated sperm per oocyte, as well as male pronucleus formation, did not differ (P>0.05) between the treatments evaluated. Under these assay conditions, especially in relation to the sperm concentration used, gamete coincubation for a period of two hours appears to be optimal for monospermy and fertilization performance. Thus, it is the optimal time period for obtaining a large number of pig embryos capable of normal development. (FIV) de suínos e se a eficiência dessa técnica poderia ser melhorada pela redução no período que os ovócitos são expostos aos espermatozoides durante a FIV. Um total de 508 (em grupos de 50) complexos cumulus-ovócito (COCs) imaturos foram maturados no meio NCSU-37. Os COCs foram cultivados por 40-44 horas e então inseminados com sêmen in natura (2.000 espermatozoides/ovócito). Os espermatozoides e ovócitos foram coincubados de acordo com os seguintes tratamentos (T): T1 = ovócitos expostos aos espermatozoides por uma hora (173 ovócitos); T2 = ovócitos expostos aos espermatozoides por duas horas (170 ovócitos) e T3 = ovócitos expostos aos espermatozoides por três horas (165 ovócitos). Após esses períodos de coincubação, os ovócitos foram lavados em meio de fertilização (meio TALP) para remoção dos espermazoides não ligados a zona pelúcida e cultivados em outro mesmo meio (não contendo espermatozoides). Após 18-20 horas de fertilização, os p...

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Timing of oviductal fluid collection, steroid concentrations, and sperm preservation method affect porcine in vitro fertilization efficiency

Cited by 31 publications

References 42 publications

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Composing the Early Embryonic Microenvironment: Physiology and Regulation of Oviductal Secretions

Improving porcine in vitro fertilization output by simulating the oviductal environment

In vitro fertilization of porcine oocytes is affected by spermatic coincubation time

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