Timing of nuclear maturation of nonstored and stored domestic cat oocytes

Kątska-Książkiewicz, L.; Ryńska, B.; Kania, Gabriela; Smorąg, Z.; Gajda, B.; Pieńkowski, Marek

doi:10.1016/s0093-691x(02)01200-1

Cited by 24 publications

(27 citation statements)

References 19 publications

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“…In the present study, there was an association between the duration of IVM culture and the proportion of feline oocytes undergoing nuclear maturation and successful IVF; the optimal culture period for IVM (in TCM-199 medium) was 30 h. Furthermore, the proportion of oocytes that reached MII was higher after 30, 36, or 48 h of IVM culture than after 24 h. Similarly, in a previous study, the maturation rate (56.0%) after 28 to 30 h of IVM was not significantly different from that (67.3%) after 42 to 45 h of IVM in TCM-199 medium [7]. Total sperm penetration rate in the present study was highest for the oocytes after 30 h of IVM, and tended to be higher than those after 36 h of IVM (P=0.16).…”

Section: Discussionsupporting

confidence: 62%

“…However, information regarding IVM of feline oocytes is fragmentary, as researchers have used various culture media and intervals for IVM. Several studies investigated the time sequence of in vitro nuclear maturation of oocytes from domestic cats [2][3][4][5][6][7]. Although the relationship between the duration of IVM culture using Eagle's minimum essential medium (MEM) and subsequent development after IVF was investigated by both Johnston et al [2] and Wolfe and Wildt [5], the timing of insemination was not consistent.…”

Section: Introductionmentioning

confidence: 99%

“…However, in these reports, there was no adequate rationale for the duration of IVM culture. Although the timing of nuclear maturation was determined in feline oocytes cultured in TCM-199 [4,7], the relationship between the duration of IVM culture and fertilization has not been studied.…”

Section: Introductionmentioning

confidence: 99%

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Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

et al. 2008

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This study was conducted to improve in vitro production of embryos from domestic cats using TCM-199 as an IVM medium. The time sequence of nuclear maturation and the optimal timing of in vitro insemination were examined. Most oocytes were at the germinal vesicle stage immediately after collection; however, 8.3% had already resumed meiosis before IVM culture. After 30 h of IVM culture, the percentage of oocytes at metaphase II (MII) reached a peak (75.5%) and did not change (P>0.05) from 30 to 48 h after IVM culture. The percentage of oocytes with two pronuclei was higher (P<0.05) for oocytes matured for 30 and 36 h (38.2 and 33.0%, respectively) than for those after IVM culture for only 24 h (18.5%).Total sperm penetration rate was highest (P<0.05) for oocytes that had been matured for 30 h (46.1%). After 30 h of IVM and 18 h of IVF culture, 66.3 and 24.8% of inseminated oocytes had cleaved and developed to the blastocyst stage, respectively. We concluded that IVM of feline oocytes for 30 h in TCM-199 resulted in optimal nuclear maturation and sperm penetration.

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Section: Discussionsupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

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Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

et al. 2008

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“…In bovine subjected to advanced ART procedure, the size of the antral follicles has an influence on the in vitro development of oocytes [11,18]. In cats, it has been reported that the in vitro maturational and developmental competence of the oocytes were influenced by season [2,25], ovarian status [2,8,9,16] and morphological quality of the cumulus and oocyte [10,16,20,29]. However, the follicular population in the cat ovaries and influence of follicular size on in vitro maturation (IVM) of cat oocytes are not clear.…”

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confidence: 99%

The Effect of Ovarian Status and Follicular Diameter on Maturational Ability of Domestic Cat Oocytes

Uchikura

Nagano

Hishinuma

2011

The Journal of Veterinary Medical Science

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ABSTRACT. The objective of this study was to clarify the effect of ovarian status and follicular size on morphological normality and maturational ability of cat oocytes. Ovarian status was classified into inactive, follicular, luteal and prepubertal, and follicles were classified into three groups according to their diameter (400-800, 800-1200 and 1200-2000 µm). In each ovarian status, the number of follicles decreased but the percentage of morphologically normal oocytes increased with the growth of follicles (p<0.05). Only a single follicle that was 1200-2000 µm in diameter was observed in two of the five prepubertal cats. In follicles that were 800-1200 µm in diameter, the percentage of normal oocytes and maturation rate were higher in prepubertal cats than in mature cats (p<0.05). Oocyte diameter tended to increase with the growth of follicles. After oocytes were cultured individually in droplets of maturation medium, the oocyte maturation rate increased with the growth of follicles in each ovarian status (p<0.05). In conclusion, oocytes collected from larger follicles possess higher maturational ability in vitro in sexually mature cats. In prepubertal cats, a higher maturation rate can be obtained from oocytes derived from small follicles compared with in mature cats.

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“…Our studies on oocyte recovery and maturation in the domestic cat led to the development of a culture system in which cat GV oocytes matured and reached metaphase II (Katska-Ksiazkiewicz et al, 2003).…”

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confidence: 99%

Animal reproduction biotechnology in Poland

Smorąg¹,

Kątska-Książkiewicz²,

Skrzyszowska³

et al. 2008

Int. J. Dev. Biol.

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The key research areas of the Department are: in vitro production of embryos, embryo cryopreservation, animal transgenesis, cloning, cytometric semen sexing and evaluation. Research has been focused on the in vitro production of animal embryos, including the development of complex methods for oocyte maturation, fertilization and embryo culture. Moreover, experiments on long-term culturing of late preantral and early antral bovine ovarian follicles have been developed. Studies on the cloning of genetically modified pigs with "humanized" immunological systems have been undertaken. A cloned goat was produced from oocytes reconstructed with adult dermal fibroblast cells. The novel technique of rabbit chimeric cloning for the production of transgenic animals was applied; additionally, the recipient-donor-cell relationship in the preimplantation developmental competences of feline nuclear transfer embryos has been studied. Regarding transgenic animal projects, gene constructs containing growth hormone genes connected to the mMt promoter were used. Modifications of milk composition gene constructs with tissue-specific promoters were performed. Moreover, pigs for xenotransplantation and animal models of human vascular diseases have been produced. Over the last 15 years, our flow cytometry research group has focused its work on new methods for sperm quality assessment and sex regulation. In the 1970s, our team initiated studies on embryo cryopreservation. As a result of vitrification experiments, the world's first rabbits and sheep produced via the transfer of vitrified embryos were born. KEY WORDS: animal cloning, transgenesis, sex regulation, cryopreservationThe Department of Biotechnology of Animal Reproduction in the National Research Institute of Animal Production was founded in 1950 as one of the institutes of the Ministry of Agriculture. During the first years of its history, the Department focused on practical issues related to insemination of cattle and horses. Prof. Wladyslaw Bielanski (Fig.1), the first Head of the Department, emphasized the practical side of research. In the mid 1960s, the Central Bank of Bull Semen was created for breeding evaluation, microbiological control of stored semen, and import and export of frozen semen.Prof. Stefan Wierzbowski, who became the Head of the Department in 1968, continued this practical approach and focused on the improvement of artificial insemination methods for various animal species, the development of semen preservation and evaluation methods, and animal behaviour and andrology.During the second half of the 1970s, the scope of the research in the Department was broadened to include aspects of female reproductive biology. Research on superovulation, embryo transfer and cryopreservation for several species of mammals was Int. J. Dev. Biol. 52: 151-155 (2008)

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Timing of nuclear maturation of nonstored and stored domestic cat oocytes

Cited by 24 publications

References 19 publications

Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes

The Effect of Ovarian Status and Follicular Diameter on Maturational Ability of Domestic Cat Oocytes

Animal reproduction biotechnology in Poland

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