The Effect of Ovarian Status and Follicular Diameter on Maturational Ability of Domestic Cat Oocytes

Uchikura, Kenzo; Nagano, Masashi; Hishinuma, Mitsugu

doi:10.1292/jvms.10-0381

Cited by 12 publications

(20 citation statements)

References 27 publications

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“…(166.2 ± 2.6 μm) in the dromedary camel (Abdoon et al., ). We found that the size of the vesicular follicle did not affect the oocyte diameter (data not shown), which disagrees with the cat (another induced ovulation mammals) (Uchikurai, Nagano, & Hishinuma, ). The diameter of the mammalian oocyte plays an essential role in the resumption of meiotic division; it is well known that oocytes with diameters of 100–110 μm gradually achieve competence to resume meiotic division.…”

Section: Discussioncontrasting

confidence: 78%

Cumulus cell expansion and first polar body extrusion during in vitro oocyte maturation in relation to morphological and morphometric characteristics of the dromedary camel ovary

Mesbah

Kafi

Nili

2016

Reprod Domestic Animals

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The morphological and morphometric characteristics of the ovary are fundamental properties for in vitro oocyte maturation. Nuclear maturation, including first polar body (1PB) extrusion, cytoplasmic maturation and cumulus cell (CC) expansion are the criteria for in vitro maturation (IVM) of oocyte. This study was designed to determine the effect of morphological and morphometric features of the ovary on CC expansion and 1PB extrusion during IVM of oocyte in the adult female dromedary camel. The weight, volume and three dimensions of ovaries from slaughtered dromedary camels and oocytes inside zona diameter and zona pellucida thickness were measured. The follicles were classified in regard to the size and oocytes according to their ooplasm appearance and CC compactness. Aspirated cumulus oocyte complexes (COCs) were incubated for 48 hr (with a 6-hr interval) in Hams-F10, and CC expansion and 1PB extrusion were assessed. Significant differences were seen in the shape, weight, volume and three dimensions of the ovaries between ≤4-year-old and >4-year-old dromedary camel (p < .5). Approximately, 95.82% of follicles were 2-4 mm in diameter. The mean (±SD) of inside zona diameter of the oocyte and zona pellucida thickness was 132.22 ± 13.8 and 14.64 ± 2.24 μm, respectively, in >4-year-old dromedary camel. The CC expansion and 1PB extrusion were seen in 86% and 21.88% of COCs, respectively. Age and sexual conditions of dromedary camel influence the morphological and morphometric characteristics of the ovary. Most COCs retrieved from 2-6 mm follicles are cultivable. The most slaughterhouse-derived COCs retrieved from 2-6 mm follicles of non-pregnant dromedary camels are excellent and good and yielding a most favourable diameter to achieve the developmental competence for IVM in an optimal time of 24-30 hr; the optimal time for CC expansion is 24-30 hr in this species. However, the CC expansion is a prerequisite process, but not sufficient for IVM.

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Section: Discussioncontrasting

confidence: 78%

Cumulus cell expansion and first polar body extrusion during in vitro oocyte maturation in relation to morphological and morphometric characteristics of the dromedary camel ovary

Mesbah

Kafi

Nili

2016

Reprod Domestic Animals

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“…() and Otoi, Murakami, Ooka, Karja, and Suzuki (). A possible explanation for these highly variable maturation rates is that the ovaries that have been used came from young and old, fat or anorectic, pregnant or pre‐pubertal cats, cats in heat or in anoestrus; all these parameters can affect the maturation rate of the obtained oocytes (Martins, Fernandes, Landim‐Alvarenga, & Lopes, ; Martins, Fernandes, Minto, Landim‐Alvarenga, & Lopes, ; Uchikura, Nagano, & Hishinuma, , ). A study from Uchikura et al.…”

Section: Introductionmentioning

confidence: 99%

Age and anti‐Müllerian hormone levels predict the success of in vitro maturation of cat oocytes

Snoeck

Sarrazin

Wydooghe

et al. 2016

Reprod Domestic Animals

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Contents Up to date, in vitro maturation (IVM) rates of oocytes are highly variable between individual cats. This study was carried out to investigate the predictive value of age and anti-Müllerian hormone (AMH) concentration in relation to capacity for IVM of cat oocytes. Ovaries were collected from 33 cats, which were divided into three age groups: (i) 0-3 months (pre-pubertal); (ii) 3-12 months (peripubertal); and (iii) older than 12 months (pubertal). The cumulus-oocyte complexes (COCs) were matured and subsequently stained to check nuclear maturation status, and blood was taken for AMH analysis. Increasing age was significantly associated with decreasing AMH levels, and mean AMH levels differed significantly between all age categories: group 1: mean AMH 18.71 μg/L; group 2: mean AMH 9.27 μg/L; and group 3: mean AMH 4.13 μg/L. Moreover, the probability of maturation was more likely in groups 2 and 3 compared to group 1. Between categories 2 and 3, no significant difference in maturation probability was found (p = .31). Finally, the probability of oocyte maturation decreased significantly with increasing AMH levels. In age group 2, oocytes with a higher AMH level were less likely to mature. In age groups 1 and 3, no significant association between the AMH level and the proportion of maturated COC was found. We can conclude that if a higher probability of nuclear maturation is required, it is preferable to use cats with lower AMH levels and older than 3 months of age to improve cat IVM.

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“…Since the estrous stage of the queens was not considered in this study, the differences in the results for Grade 3 oocytes among the experiments may have been due to the use of oocytes at different stages of the estrous cycle. Moreover, another group [26] has suggested that the size of the follicles/oocytes, as well as their qualities, may affect the in vitro maturation and subsequent development of cat oocytes. Therefore, future studies should evaluate the effects of the stage of the estrous cycle, as well as oocyte diameter, on the development of Grade 3 oocytes.…”

Section: Discussionmentioning

confidence: 99%

Feline embryo development in commercially available human media supplemented with fetal bovine serum

Alam

Iwata

Fujiki

et al. 2019

The Journal of Veterinary Medical Science

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Feline embryo development was examined for 7 days after fertilization using commercially available human media supplemented with 0.3% bovine serum albumin (BSA) or 5% fetal bovine serum (FBS). Cumulus-oocyte complexes were categorized as Grades 1, 2, and 3 according to morphology. Only-One Medium (OM) was used for in vitro culture (IVC) in OM + BSA, OM + FBS, and OM + BSA/FBS, with BSA supplementation for the first 2 days and FBS for the subsequent 5 days. Embryos cultured in Early Culture Medium (1–2 days) and Blastocyst Medium (3–7 days) were defined as EB + BSA and EB + BSA/FBS. The developmental rate until the blastocyst stage of Grade 1 and 2 oocytes cultured in OM + BSA/FBS was higher than for the other groups and was significantly higher than for the OM + BSA and EB + BSA groups ( P <0.01). Grade 3 oocytes cultured in OM + BSA/FBS also showed the greatest proportion of blastocyst formation. However, FBS supplementation throughout the IVC period reduced blastocyst number. The percentage of 2 pronuclei after fertilization as well as blastocyst cell number were significantly higher in Grade 1 and 2 than Grade 3 oocytes when cultured in OM + BSA/FBS ( P <0.05). These results indicate that commercially available OM supplemented with BSA for the first 2 days of culture and FBS for the subsequent 5 days is suitable for feline embryo development until the blastocyst stage.

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The Effect of Ovarian Status and Follicular Diameter on Maturational Ability of Domestic Cat Oocytes

Cited by 12 publications

References 27 publications

Cumulus cell expansion and first polar body extrusion during in vitro oocyte maturation in relation to morphological and morphometric characteristics of the dromedary camel ovary

Cumulus cell expansion and first polar body extrusion during in vitro oocyte maturation in relation to morphological and morphometric characteristics of the dromedary camel ovary

Age and anti‐Müllerian hormone levels predict the success of in vitro maturation of cat oocytes

Feline embryo development in commercially available human media supplemented with fetal bovine serum

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