Timing of insemination using sex-sorted sperm in embryo production with Bos indicus and Bos taurus superovulated donors

Soares, J. G.; Martins, C. M.; Carvalho, N. A. T.; Nicacio, Alessandra Corallo; Abreu-Silva, Ana Lúcia; Filho, E. P. Campos; Júnior, J. R. S. Torres; Filho, Manoel Francisco de Sá; Baruselli, Pietro Sampaio

doi:10.1016/j.anireprosci.2011.08.003

Cited by 31 publications

(26 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results have been reported previously (Kaimio et al, 2013). These results were observed even when greater sperm concentrations (10 x 10 6 and 20 x 10 6 ) were used in heat detection AI (Sartori et al, 2004), when timed AI (TAI) was used in heifers that were superovulated without an ovulation inductor (Schenk et al, 2006), when AI was conducted 6 h after the pre-established time (Soares et al, 2011), and when four inseminations were done with estrus observation (Larson et al, 2010).…”

Section: Discussionsupporting

confidence: 73%

“…Although the sperm sex-sorting process has significantly evolved, when it is used in superovulated cows the fertilization rate is lower than when using nonsorted semen (Sartori et al, 2004;Larson et al, 2007Larson et al, , 2010Soares et al, 2011;Kaimio et al, 2013). This result can be explained by the low sperm concentration and damage caused by the sorting process followed by freezing (dilution, laser exposure, process speed and centrifugation) (Schenk et al, 1999).…”

Section: Discussionmentioning

confidence: 80%

“…Several studies have reported the use of different strategies for increasing the results of artificial insemination (AI) using sex-sorted semen in superovulated donors. These strategies include using different sperm concentrations (Sartori et al, 2004;Schenk et al, 2006), using fresh sex-sorted semen (Hayakawa et al, 2009), using a higher number of AIs (Peippo et al, 2009;Larson et al, 2010) and using AI at different times (Soares et al, 2011). The use of sexsorted semen in superovulated cattle is promising when considering the limitations of in vitro embryo production related to cryopreservation (Viana and Camargo, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fertilization rate and embryo production of superovulated dairy cows after insemination with non-sorted and sex-sorted semen

et al. 2016

View full text Add to dashboard Cite

The aim of this study was to evaluate the fertilization rate of cows that were superovulated and artificially inseminated with sex-sorted semen. Cows were treated with an intravaginal progesterone device plus estradiol benzoate (day 0). Superstimulation treatments began four days after with eight applications of FSH at 12 h intervals. D-Cloprostenol was administered on day 6. Progesterone device was removed on day 7, and LH was administered on day 8. The treatments were divided as follows: NonSx, two AI with non-sorted semen were conducted 12 and 24 h after LH; Sx12&24, two AI with sex-sorted semen were conducted 12 and 24 h after LH; and Sx24&36, two AI with sex-sorted semen were conducted 24 and 36 h after LH. Embryos were recovered on day 16 and were evaluated and classified. Percentage of fertilized embryos tended to be greater for the non-sorted semen than the sex-sorted semen. The number of unfertilized oocytes was smaller when the non-sorted semen was used relative to the sex-sorted semen. There was no difference between the treatments that used sexed semen. In conclusion, the use of sex-sorted semen in superovulated dairy cows results in greater numbers of unfertilized oocytes than non-sorted. However, when only sorted semen is used AI should be performed 24 and 36 h after LH.

show abstract

Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fertilization rate and embryo production of superovulated dairy cows after insemination with non-sorted and sex-sorted semen

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In cattle, approximately 5000 unsorted sperm [23] and as few as 600 [24] or 1500 to 2250 sexed sperm [4] are sufficient to fertilize a bovine oocyte in vitro, whereas 20 million unsorted sperm [25] and 1 to 4 million of sorted and frozen sperm [13,26] are normally used per AI. In vitro production (IVP) of embryos with sex-sorted sperm could justify the use of this sperm despite its higher price.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

et al. 2012

View full text Add to dashboard Cite

In this work we analyzed the effects of three culture systems on developmental ability of bovine embryos in vitro produced with sexed sperm, the survival to vitrification (cryologic vitrification method) of such blastocysts, and their pregnancy rates after embryo transfer to recipients, both as fresh and after vitrification/warming. Finally, we measured the accuracy of the sorting protocol by a polymerase chain reaction-based method to validate the embryo sex at blastocyst stages. We confirmed an individual effect of the bull as well as development rates of embryos produced with sorted sperm lower than embryos with unsorted sperm, independent of the culture system used. The cryoresistance to vitrification of embryos produced with sexed sperm did not differ from that of conventionally produced embryos (re-expansion rates at 24 and 48 h: 74.6% vs. 75.5%, and 64.5% vs. 68.1% for embryos produced with conventional and sorted sperm, respectively; hatching rates at 48 h: 63.55% vs. 55.5% for embryos produced with conventional and sorted sperm, respectively). Finally, no significant differences were found in pregnancy rates after the embryo transfer of fresh and vitrified/warmed blastocysts (52.8% vs. 42.0%, respectively; P Ͼ 0.05). Male and female embryos produced with sorted sperm showed the same quality in terms of developmental ability, cryoresistance, and pregnancy rates after transfer. Our culture system, coupled with the vitrification in fiber plugs, provides good quality sex-known embryos which survive vitrification at similar rates than embryos produced with conventional unsorted sperm; also it produces good pregnancy rates after transfer of sexed embryos both fresh and after vitrification and warming.

show abstract

“…The advantage of this later technology is the possibility to produce female-sexed embryos without losses related to failure in fertilization and poorer quality commonly reported while utilizing sexed semen in superovulated cows (Soares et al, 2011). Hence, IVP is gaining ground compared to standard in vivo embryo production mainly because of its greater efficiency in terms of embryo production numbers that can be retrieved out of the same donor cow.…”

Section: Use Of Moet and Ivp In Dairy Herdsmentioning

confidence: 99%

Genetic market in cattle (Bull, AI, FTAI, MOET and IVP): financial payback based on reproductive efficiency in beef and dairy herds in Brazil

Baruselli¹,

A²,

M³

et al. 2018

Anim Reprod

View full text Add to dashboard Cite

A number of reproductive biotechnologies are currently available to multiply offspring from high genetic merit animals to enhance reproductive efficiency and profitability both in dairy and beef herds. Some of these technologies such as fixed time artificial insemination (FTAI), when correctly implemented, generally allow greater reproductive performance than natural breeding. Besides the use of frozen-thawed semen during artificial insemination, cattle recipients can also be synchronized to receive embryos (produced in vivo or in vitro) at set dates with fertility results that usually outperforms natural breeding as well as artificial insemination (AI), particularly during warm seasons and in repeat breeders cows. Altogether, the use of hormonal programs to synchronize ovulation time simplify field routine, can easily fix physiological limitations related to postpartum anestrus (beef cows), poor estrus detection efficiency due to less evident estrus signs (dairy cows), making AI and ET viable to commercial herds both in terms of results and economical returns.

show abstract

Timing of insemination using sex-sorted sperm in embryo production with Bos indicus and Bos taurus superovulated donors

Cited by 31 publications

References 20 publications

Fertilization rate and embryo production of superovulated dairy cows after insemination with non-sorted and sex-sorted semen

Fertilization rate and embryo production of superovulated dairy cows after insemination with non-sorted and sex-sorted semen

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

Genetic market in cattle (Bull, AI, FTAI, MOET and IVP): financial payback based on reproductive efficiency in beef and dairy herds in Brazil

Contact Info

Product

Resources

About