Timing and duration of nursing from birth affect neonatal porcine uterine matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1

Ho, Teh‐Yuan; Rahman, Kathleen M.; Camp, Meredith E.; Wiley, Anne A.; Bartol, Frank F.; Bagnell, Carol A.

doi:10.1016/j.domaniend.2016.10.002

Cited by 10 publications

(14 citation statements)

References 38 publications

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“…Evidence that lactocrine deficiency on the day of birth leads to permanent impairment of reproductive performance in adult female pigs, as reflected by reduced uterine capacity to support large, viable litters (Vallet et al 2015), suggests that lactocrine signaling from birth affects the porcine uterine developmental program rapidly. Consistently, when colostrum consumption was delayed 0.5 h from birth or limited to 12 h from birth, markers of female reproductive tract (FRT) development, including uterine matrix metalloproteinase-9 (MMP9) and tissue inhibitor of metalloproteinase-1 (TIMP1), as well as cervical MMP9 expression levels on PND 2 were similar to those observed for nursed gilts that consumed colostrum continuously for 48 h from birth (Ho et al 2017). Further, a single feeding of colostrum at birth was sufficient to support normal levels of cervical cell proliferation at 12 h postnatal (Camp et al 2014).…”

Section: Introductionmentioning

confidence: 59%

“…Analysis of the porcine uterine transcriptome between birth and PND 2 identified age-and lactocrinesensitive transcripts within the plasminogen-activating network, including MMPs and TIMPs (Rahman et al 2016). Further, a recent study determined that nursing for 12 h from birth was sufficient for the expression of uterine MMP9 and TIMP1, as well as cervical MMP9, at levels equivalent to those observed in gilts nursed for 48 h from birth (Ho et al 2017). Consistently, nursing from birth until 12 h is required for normal uterine expression of MMP9 and TIMP1.…”

Section: Discussionmentioning

confidence: 93%

“…Uterine proteins (20 μg) were resolved on 12% total monomer, NuPAGE Bis-Tris gels (Invitrogen) under reducing conditions, followed by transfer onto nitrocellulose membranes (0.45 μm pore size; Bio-Rad Laboratories). Relative abundance of uterine MMP9 (pro 92 kDa, active 84 kDa), MMP2 (pro 72 kDa, active 66 kDa) and TIMP1 (28 kDa) proteins were determined by immunoblotting and densitometry using actin (43 kDa) as the loading control (Ho et al 2017).…”

Section: Protein Extraction and Immunoblot Analysesmentioning

confidence: 99%

“…The membranes were incubated with either mouse anti-human MMP2 (1:1000; IM33; EMD Millipore), mouse anti-human MMP9 (1:100; IM09L; EMD Millipore), rabbit anti-human TIMP1 (1:1000; C20; Santa Cruz Biotechnology) or goat anti-human actin antibody (1:3000, C11; Santa Cruz Biotechnology) in TBST-5% NFMP overnight at 4°C. Antibodies directed against human MMP2, MMP9 and TIMP1 were validated for use in the neonatal pig uterus and cervix (Ho et al 2017).…”

Section: Protein Extraction and Immunoblot Analysesmentioning

confidence: 99%

“…Blots were washed with TBST and incubated with infrared fluorescent dye-conjugated secondary antibodies (1:20,000; LI-COR Biosciences, Lincoln, NE, USA) in TBST-5% NFMP for 1 h at room temperature and proteins were visualized with the Odyssey Clx infrared imaging system (LI-COR Biosciences). Positive controls for MMP2, MMP9 and TIMP1 immunoblots consisted of pregnant sow uterine tissue (gestation day 90), as described by Ho et al (2017). Negative controls consisted of primary antibody substitution with an irrelevant mouse IgG (for MMP2 and MMP9) or rabbit IgG (for TIMP1), in each case as appropriate.…”

Section: Protein Extraction and Immunoblot Analysesmentioning

confidence: 99%

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Effects of colostrum, feeding method and oral IGF1 on porcine uterine development

George¹,

Rahman²,

Miller³

et al. 2018

Reproduction

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Nursing ensures lactocrine delivery of maternally derived, milk-borne bioactive factors to offspring, which affects postnatal development of female reproductive tract tissues. Disruption of lactocrine communication for two days from birth (postnatal day (PND) 0) by feeding milk replacer in lieu of nursing or consumption of colostrum alters porcine uterine gene expression globally by PND 2 and inhibits uterine gland genesis by PND 14. Here, objectives were to determine effects of: (1) nursing or milk replacer feeding from birth; (2) a single dose of colostrum or milk replacer and method of feeding and (3) a single feeding of colostrum or milk replacer, with or without oral supplementation of IGF1, administered at birth on aspects of porcine uterine development at 12-h postnatally. Results indicate nursing for 12 h from birth supports rapid establishment of a uterine developmental program, illustrated by patterns of endometrial cell proliferation, expression of genes associated with uterine wall development and entry into mitosis and establishment of a uterine MMP9/TIMP1 system. A single feeding of colostrum at birth increased endometrial cell proliferation at 12 h, regardless of method of feeding. Oral supplementation of IGF1 was sufficient to support endometrial cell proliferation at 12 h in replacer-fed gilts, and supplementation of colostrum with IGF1 further increased endometrial cell proliferation. Results indicate that lactocrine regulation of postnatal uterine development is initiated with the first ingestion of colostrum. Further, results suggest IGF1 may be lactocrine-active and support a 12-h bioassay, which can be used to identify uterotrophic lactocrine activity.

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Section: Introductionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 93%

Section: Protein Extraction and Immunoblot Analysesmentioning

confidence: 99%

Section: Protein Extraction and Immunoblot Analysesmentioning

confidence: 99%

Section: Protein Extraction and Immunoblot Analysesmentioning

confidence: 99%

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Effects of colostrum, feeding method and oral IGF1 on porcine uterine development

George¹,

Rahman²,

Miller³

et al. 2018

Reproduction

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Maternal lactocrine programming of porcine reproductive tract development

Bagnell

George

et al. 2017

Molecular Reproduction Devel

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The lactocrine hypothesis for maternal programming of female reproductive tract development is based on the idea that non-nutritive, milk-borne bioactive factors (MbFs), delivered from mother to offspring during nursing, play a role in determining the trajectory of development with long-term consequences in the adult. Porcine female reproductive tract development is completed postnatally, and the period during which maternal support of neonatal growth derives exclusively from colostrum/milk defines a window of opportunity for lactocrine programming of reproductive tissues. Beyond nutrition, milk serves as a delivery system for a variety of bioactive factors. Porcine relaxin is a prototypical MbF. Present in colostrum at highest concentrations at birth, relaxin is transmitted into the circulation of nursing piglets where it can act on Relaxin receptors found in neonatal female reproductive tract tissues. This process is facilitated by the physiology of the maternal-neonatal dyad and the fact that the neonatal gastrointestinal tract is open to absorb macromolecules for a period of time postnatally. Age at first nursing and duration of nursing from birth are also important for porcine female reproductive tract development. These parameters affect both the quality and quantity of colostrum consumed. Disruption of lactocrine signaling by feeding milk replacer from birth altered porcine uterine, cervical, and testicular development by postnatal Day 2. Moreover, insufficient colostrum consumption in nursing piglets can impair uterine capacity to support viable litters of optimal size in adulthood. In the pig, lactocrine signaling supports neonatal organizational events associated with normal reproductive development and may program adult uterine capacity.

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Effect of low colostrum intake on gastrointestinal development and uterine and cervical morphometrical architecture in the neonatal gilt

Langendijk¹,

Fleuren²,

Venrooy³

et al. 2023

animal

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Timing and duration of nursing from birth affect neonatal porcine uterine matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1

Cited by 10 publications

References 38 publications

Effects of colostrum, feeding method and oral IGF1 on porcine uterine development

Effects of colostrum, feeding method and oral IGF1 on porcine uterine development

Maternal lactocrine programming of porcine reproductive tract development

Effect of low colostrum intake on gastrointestinal development and uterine and cervical morphometrical architecture in the neonatal gilt

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