Time-varying magnetic fields increase cytosolic free Ca2+ in HL-60 cells

Carson, Jeffrey J. L.; Prato, Frank S.; Drost, Dick J.; Diesbourg, L.D.; Dixon, S. Jeffrey

doi:10.1152/ajpcell.1990.259.4.c687

Cited by 140 publications

(61 citation statements)

References 22 publications

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“…Experiments with different cell types consistently point to the Ca 2ϩ signaling system as the primary cellular target of MF interactions (5,10,23,52,53,60,69,86,87). In many cases, alternating MFs alone or in combination with static fields increase intracellular Ca 2ϩ , as, for instance, in HL60 cells (10), T-lymphocytes and leukemia cells (60), the human T cell line (Jurkat) (55,56), bone cells (23), pituitary cells (5), and human astrocytoma cells (69).…”

Section: Physiological Effects Of Mf Exposurementioning

confidence: 99%

Cellular target of weak magnetic fields: ionic conduction along actin filaments of microvilli

Gartzke

Lange²

2002

American Journal of Physiology-Cell Physiology

102

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Gartzke, Joachim, and Klaus Lange. Cellular target of weak magnetic fields: ionic conduction along actin filaments of microvilli. Am J Physiol Cell Physiol 283: C1333-C1346, 2002; 10.1152/ajpcell. 00167.2002.-The interaction of weak electromagnetic fields (EMF) with living cells is a most important but still unresolved biophysical problem. For this interaction, thermal and other types of noise appear to cause severe restrictions in the action of weak signals on relevant components of the cell. A recently presented general concept of regulation of ion and substrate pathways through microvilli provides a possible theoretical basis for the comprehension of physiological effects of even extremely low magnetic fields. The actin-based core of microfilaments in microvilli is proposed to represent a cellular interaction site for magnetic fields. Both the central role of F-actin in Ca 2ϩ signaling and its polyelectrolyte nature eliciting specific ion conduction properties render the microvillar actin filament bundle an ideal interaction site for magnetic and electric fields. Ion channels at the tip of microvilli are connected with the cytoplasm by a bundle of microfilaments forming a diffusion barrier system. Because of its polyelectrolyte nature, the microfilament core of microvilli allows Ca 2ϩ entry into the cytoplasm via nonlinear cable-like cation conduction through arrays of condensed ion clouds. The interaction of ion clouds with periodically applied EMFs and field-induced cation pumping through the cascade of potential barriers on the F-actin polyelectrolyte follows well-known physical principles of ion-magnetic field (MF) interaction and signal discrimination as described by the stochastic resonance and Brownian motor hypotheses. The proposed interaction mechanism is in accord with our present knowledge about Ca 2ϩ signaling as the biological main target of MFs and the postulated extreme sensitivity for coherent excitation by very low field energies within specific amplitude and frequency windows. Microvillar F-actin bundles shielded by a lipid membrane appear to function like electronic integration devices for signal-tonoise enhancement; the influence of coherent signals on cation transduction is amplified, whereas that of random noise is reduced. calcium signaling; cell differentiation; Brownian motor hypothesis; cyclotron resonance; hair cell; mechanoelectrical coupling; physiological effects MORE THAN A DECADE OF RESEARCH on field effects in biological systems has yielded rather compelling data for the involvement of the Ca 2ϩ signaling pathway as a primary and main target of magnetic fields (MFs). As first demonstrated by Liburdy and colleagues (52,53) and later on by a number of other authors, the Ca 2ϩ influx pathways of isolated or cultured cells are severely affected by rather low MF energies.The physiological relevance of this finding is high, because Ca 2ϩ represents the most important intracellular signal governing almost all physiological functions in differentiated cells. Most importantly, cytopla...

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Section: Physiological Effects Of Mf Exposurementioning

confidence: 99%

Cellular target of weak magnetic fields: ionic conduction along actin filaments of microvilli

Gartzke

Lange²

2002

American Journal of Physiology-Cell Physiology

102

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“…The physical coupling between calcium signaling and EMFs could be the result of ion-protein interactions [38,39] or from the effect of the induced electric currents in the signaling [40]. Many authors propose that calcium itself as an ion is the primary target of EMFs during the initial step of coupling [41,42,43,44]. In addition, other forms of stimulation where calcium provokes the activation of downstream signaling pathways include exercise in the form of muscle stretch [45,46] and shear stress through fluid flow [47,48].…”

Section: Introductionmentioning

confidence: 99%

Calcium's Role in Mechanotransduction during Muscle Development

Damm

Egli

2014

Cell Physiol Biochem

11,389

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Mechanotransduction is a process where cells sense their surroundings and convert the physical forces in their environment into an appropriate response. Calcium plays a crucial role in the translation of such forces to biochemical signals that control various biological processes fundamental in muscle development. The mechanical stimulation of muscle cells may for example result from stretch, electric and magnetic stimulation, shear stress, and altered gravity exposure. The response, mainly involving changes in intracellular calcium concentration then leads to a cascade of events by the activation of downstream signaling pathways. The key calcium-dependent pathways described here include the nuclear factor of activated T cells (NFAT) and mitogen-activated protein kinase (MAPK) activation. The subsequent effects in cellular homeostasis consist of cytoskeletal remodeling, cell cycle progression, growth, differentiation, and apoptosis, all necessary for healthy muscle development, repair, and regeneration. A deregulation from the normal process due to disuse, trauma, or disease can result in a clinical condition such as muscle atrophy, which entails a significant loss of muscle mass. In order to develop therapies against such diseased states, we need to better understand the relevance of calcium signaling and the downstream responses to mechanical forces in skeletal muscle. The purpose of this review is to discuss in detail how diverse mechanical stimuli cause changes in calcium homeostasis by affecting membrane channels and the intracellular stores, which in turn regulate multiple pathways that impart these effects and control the fate of muscle tissue.

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“…The calcium ion is an important intracellular signal that is involved in many cellular pathways. It is associated with lymphocyte activation (10,25), and many studies have reported the biological effects of static or pulsed magnetic fields on membrane ion transport. The influence of magnetic fields on the movement of calcium ion in the context of membranes could explain the diversity and variety of cellular responses observed in cell proliferation, cell differentiation and release of cytokines.…”

Section: Discussionmentioning

confidence: 99%