Time-sequence histologic imaging of laser-treated cherry angiomas with in vivo confocal microscopy

Aghassi, David; Anderson, R. Rox; González, Salvador

doi:10.1067/mjd.2000.105560

Cited by 78 publications

(55 citation statements)

References 13 publications

(14 reference statements)

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“…In particular, these techniques have been widely applied to vascular lesions, targeting the erythrocytes that contain the chromophore molecule hemoglobin (15). Neutrophils are dominant in the early inflammatory response (16). Our in vitro system is simpler in that damaged adjacent tissue (endothelium, blood vessel wall) is not present, and ingress of neutrophils is attributable solely to chemoattraction from the lysed erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

Tonic inhibition of chemotaxis in human plasma

Malawista

Chevance²,

Damme

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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We found exaggerated chemotaxis in plasma treated with EDTA and thought that the EDTA might itself be inhibiting a tonic inhibitor(s) of chemotaxis. Our plasma fractionations suggested that evidence should be sought for a lipid moiety carrying this activity, and on spectrometry (LC-MS-MS together with GC-MS analyses), the biologically active but not the inactive fraction contained oleic and arachidonic acids. Because fatty acids are largely protein bound, we flooded plasma preparations with delipidated albumin, reasoning that it would bind enough fatty acids, including inhibitory ones, to counter their tonic inhibition. Indeed, we observed dramatic increases in chemotaxis. Hence, adding delipidated albumin to plasma has a similar effect to that of adding EDTA-amplification of the chemotactic response. Oleic acid in physiologic concentrations diminishes the magnifying effects of both EDTA and of delipidated albumin, and in fact diminishes the chemotactic response even without the presence of the amplifiers of chemotaxis. In contrast, arachidonic acid amplifies further the effect of EDTA but not of delipidated albumin, and this augmentation appears to be caused by an EDTA-dependent enrichment of the chemotactic gradient with leukotriene B4 (LTB4). We conclude that oleic acid, the blood levels of which vary among individuals, is at least one tonic inhibitor of chemotaxis in plasma.fatty acids ͉ oleic acid ͉ neutrophils T his work began with the videomicroscopic observation that polymorphonuclear leukocytes (PMNs) from blood anticoagulated with EDTA, but not with heparin, segregate into groups, usually around a monocyte (1, 2). The EDTA effect occurred in serum as well as in plasma (2). When the monocytes became less adhesive and moved from the center of the field, the PMNs did not follow. Rather, they ignored the monocyte, remaining focused on the place where it used to be, and often continuing to arrive in large numbers; they appeared to have taken over the generation of a chemotactic gradient, replacing the one initially provided by the adherent monocyte (2). Moreover, this prolonged chemoattraction did not depend on triggering by monocytes; we saw large aggregates accrue around PMNs containing ingested material, damaged (motionless, mummified appearance) PMNs, or detritus in the slide preparation.To pursue the phenomenon of prolonged chemotaxis in EDTA/ plasma in a more systematic approach, we used the chemoattractant gradient established by an erythrocyte destroyed by laser microirradiation (3, 4). With videomicroscopy, one can observe directly and continuously the behavior of PMN in real time before, during, and after establishment of a chemotactic gradient. Within several seconds of the laser flash, PMNs in the area turn in the direction of the newly created chemotactic target. PMNs then move to the target, and surround it for several minutes, before departing cells begin to outnumber arriving ones, presumably mirroring a decline in the exudation of chemoattractant. As in the case of chemotactic gradients ini...

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Section: Discussionmentioning

confidence: 99%

Tonic inhibition of chemotaxis in human plasma

Malawista

Chevance²,

Damme

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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“…Reflectance confocal-laser-scanning microscopy (CLSM) has been introduced as a technique to qualitatively and quantitatively assess the epidermal-dermal pattern in human skin up to a depth of 350 lm (Aghassi et al, 2000;Gonzalez et al, 1999a;Rajadhyaksha et al, 1999). Until now, this high resolution technique was used for observation of cutaneous morphology on cellular and subcellular levels in vivo and in real time.…”

Section: Introductionmentioning

confidence: 99%

Reflectance confocal‐laser‐scanning microscopy in vivo assessments of cigarette‐induced dynamic alterations of cutaneous microcirculation on histomorphological level

Altintas

Altıntaş

Guggenheim

et al. 2008

Microscopy Res & Technique

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“…17 In vivo confocal microscopy imaging preserves the natural architecture of the tissue, including cellular hydration, natural tonicity, and, thus, the natural contrast of structures, 17,19 while enabling the assessment of details in the same tissue over time. [20][21][22] With this technique, normal melanocytes, as well as abnormalities such as eccentric nuclei and loss of keratinocytic demarcation, may be discernible on human skin. However, in some instances, it may be difficult to differentiate between melanocytes and pigmented keratinocytes.…”

Section: Commentmentioning

confidence: 99%

Use of In Vivo Confocal Microscopy in Malignant Melanoma

Curiel‐Lewandrowski¹,

Williams²,

Swindells³

et al. 2004

Arch Dermatol

126

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Background: Melanomas with poorly defined borders, lack of pigmentation, lentiginous extension, and location in cosmetically sensitive regions represent diagnostic and therapeutic challenges. Repeated surgical reexcisions are frequently required to achieve tumorfree margins. The use of reflectance mode confocal microscopy as an noninvasive method has shown to be a promising tool for diagnosing pigmented lesions in vivo.Observations: We report 3 clinical cases of melanoma: amelanotic melanoma (case 1), locally recurrent melanoma (case 2), and lentigo maligna melanoma (case 3). In case 1, in vivo confocal microscopy was instru-mental in making the diagnosis and in monitoring the response to imiquimod therapy for in situ residual disease. It was also used to successfully delineate preoperative surgical margins in cases 2 and 3.

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Time-sequence histologic imaging of laser-treated cherry angiomas with in vivo confocal microscopy

Cited by 78 publications

References 13 publications

Tonic inhibition of chemotaxis in human plasma

Tonic inhibition of chemotaxis in human plasma

Reflectance confocal‐laser‐scanning microscopy in vivo assessments of cigarette‐induced dynamic alterations of cutaneous microcirculation on histomorphological level

Use of In Vivo Confocal Microscopy in Malignant Melanoma

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