Use of In Vivo Confocal Microscopy in Malignant Melanoma

Curiel‐Lewandrowski, Clara; Williams, Christy M.; Swindells, Kirsty J.; Tahan, Steven R.; Astner, Susie; Frankenthaler, Robert A.; González, Salvador

doi:10.1001/archderm.140.9.1127

Cited by 126 publications

(23 citation statements)

References 19 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its mode of action implies the induction of the innate immunity resulting in a selective antitumoral immune response through the activation of dendritic cells, cytotoxic T lymphocytes and proinflammatory cytokines. RCM has been used for monitoring and evaluation of treatment efficacy of basal cell carcinoma and lentigo maligna melanoma during topical IMIQ therapy [36,37,38]. To our knowledge, no systematic RCM evaluation of treatment response of AK to IMIQ has been performed.…”

Section: Discussionmentioning

confidence: 99%

Reflectance Confocal Microscopy for Noninvasive Monitoring of Therapy and Detection of Subclinical Actinic Keratoses

et al. 2009

View full text Add to dashboard Cite

Background: Actinic keratoses (AK) represent cutaneous carcinoma in situ and have previously been evaluated by reflectance confocal microscopy (RCM). Treatment of AK with imiquimod (IMIQ) 5% cream has been shown to ‘highlight’ subclinical lesions. Objective: The aim of this study was to test the applicability of RCM for noninvasive monitoring of actinic field cancerization and detection of subclinical AK. Subjects and Methods: AK and surrounding skin sites with no apparent AK of 11 volunteers were selected for imaging and subsequently classified as ‘clinical’ and ‘subclinical’ AK. IMIQ was used 3 times weekly for 4 weeks. Results: RCM was able to detect morphologic features of AK in both clinical and subclinical AK; features were more pronounced in clinical lesions. The immunomodulatory response induced by IMIQ was visualized by RCM. Conclusion: Our findings indicate that RCM allows noninvasive monitoring of treatment response in vivo and permits early detection of subclinical AK, thus substantiating the incentive for therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Reflectance Confocal Microscopy for Noninvasive Monitoring of Therapy and Detection of Subclinical Actinic Keratoses

et al. 2009

View full text Add to dashboard Cite

show abstract

“…The examination of the distribution and morphology of melanocytes under in vivo reflectance confocal microscopy (RCM) improves our ability to recognize early melanoma at the bedside 1–5 . As with any imaging modality, however, RCM evaluation may give rise to false-positive or false-negative results, discordant with the final histopathologic diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Langerhans cells and melanocytes share similar morphologic features under in vivo reflectance confocal microscopy: A?challenge for melanoma diagnosis

Hashemi

Pulitzer

Scope

et al. 2012

Journal of the American Academy of Dermatology

View full text Add to dashboard Cite

Background Intraepidermal Langerhans cells (ILC) are difficult to differentiate from melanocytes under reflectance confocal microscopy (RCM) and their presence may simulate pagetoid spread of melanocytes on RCM images. Objective To correlate bright round and dendritic cells in a pagetoid pattern identified on RCM with findings of conventional histopathology and immunohistochemistry for lesions that were falsely diagnosed as melanoma by RCM. Methods This retrospective study included histopathologically proven nevi, imaged by RCM, which displayed bright cells in a pagetoid pattern (BCPP) under RCM, resulting in the incorrect RCM diagnosis of melanoma. Morphological comparisons were histopathologically proven melanomas displaying BCPP on RCM and biopsy-proven nevi without such cells on RCM. Results We identified 24 nevi that were falsely diagnosed as melanoma by RCM due to the presence of BCPP. These pagetoid cells on RCM corresponded on histopathology to ILC with a high density in 23 of the 24 nevi (95%) and to melanocytes in 7 of the 24 nevi (29%). Among 6 melanomas displaying BCPP on RCM, ILC with high density were observed histopathologically in 5 of the 6 cases (83%) and pagetoid melanocytes were seen in all 6 cases (100%). Limitations The results cannot be generalized to clinically banal-appearing nevi. Conclusions Although the finding of BCPP is a useful RCM feature for the diagnosis of melanoma it does not always imply the presence of pagetoid melanocytes but may at times, represent ILC.

show abstract

“…Increased clinical application of CLSM is anticipated [15,[23][24][25][26][27]. In this study, reconstructed images of given sections were converted into three-dimensions using the data set of a large number of tomograms in the horizontal directions.…”

Section: Discussionmentioning

confidence: 99%

The real-time, three-dimensional analyses of benign and malignant skin tumors by confocal laser scanning microscopy

Ono

Sakemoto

Ogino

et al. 2006

Journal of Dermatological Science

View full text Add to dashboard Cite

Use of In Vivo Confocal Microscopy in Malignant Melanoma

Cited by 126 publications

References 19 publications

Reflectance Confocal Microscopy for Noninvasive Monitoring of Therapy and Detection of Subclinical Actinic Keratoses

Reflectance Confocal Microscopy for Noninvasive Monitoring of Therapy and Detection of Subclinical Actinic Keratoses

Langerhans cells and melanocytes share similar morphologic features under in vivo reflectance confocal microscopy: A?challenge for melanoma diagnosis

The real-time, three-dimensional analyses of benign and malignant skin tumors by confocal laser scanning microscopy

Contact Info

Product

Resources

About