Time-resolved luminescence detection of derivatized thiols in column liquid chromatography

Design of Lanthanide Complex Probes for Highly Sensitive Time-Resolved Fluorometric Detection Methods and Its Application to Biochemical, Environmental and Clinical Analyses

“…For analyses of compounds that do not form fluorescent complexes with lanthanide ions, precolumn derivatization reagents have been reported [56][57][58][59][60][61]. In the time-resolved fluorometric HPLC system, a new time-resolved fluorometric HPLC detector and a new b -diketonate europium chelate reagent have been developed and it has achieved highly sensitive determination.…”

“…Three HPLC detectors equipped with time-resolved fluorometric measurement mode have been reported previously [32,41,[56][57][58][59][60][61]. Among them, a block diagram of timeresolved fluorometric HPLC detector (Hamamatsu Photonics, Japan) is shown in Fig.…”

Liquid Chromatographic Determination Using Lanthanides as Time-Resolved Luminescence Probes for Drugs and Xenobiotics: Advantages and Limitations

“…To extend the applicability, a pre-column labelling reaction for thiols containing analytes was developed. [18][19][20] The idea is to derivatize the analyte to a product that, first, is able to form a stable complex with Ln 3+ and, second, sensitize the lanthanide luminescence. A commercially available derivatization reagent for thiol-containing analytes, i.e., 4-maleimidosalicylic acid (4-MSA), was chosen because the salicylate group has sensitizing properties for Tb 3+ .…”

“…In particular, for intracellular determinations low limits of detection (LOD) ( < 10 28 mol l 21 ) will be crucial in the future but at the moment this requirement seems difficult to fulfil systematically. In fact, sensitized lanthanide luminescence used in HPLC separations has been developed for several drugs such as NSAIDs, 8 ciprofloxacin, 9 bleomycin, 11 orotate, 15 tetracyclines, 12 nucleic acids and nucleotides, 10 steroids, 14 theophylline, 16 mycotoxines 17 and thiol compounds, [18][19][20] with the aim of reaching this LOD and improving the selectivity of the analyte.…”

“…7 Regarding drug and xenobiotic determinations, several papers using HPLC and Ln 3+ sensitized luminescence have been published on non-steroidal antiinflammatory agents (NSAIDs), 8 ciprofloxacin, 9 nucleic acids, 10 bleomycin, 11 tetracycline, 12,13 steroids, 14 orotate, 15 theophylline, 16 mycotoxines 17 and thiol compounds. [18][19][20] The objective of this review is to analyse constraints related to Ln 3+ properties and HPLC and emphazise the advantages and limitations of such a technique. We shall discuss the use of normal-phase HPLC (NP-HPLC) and reversed phase HPLC (RP-HPLC) and the mode of addition of lanthanide salts either in pre-or post-column.…”