Time resolved laser-induced fluorescence of electrosprayed ions confined in a linear quadrupole trap

Friеdrich, J.; Fu, Jinmei; Hendrickson, Christopher L.; Marshall, Alan G.; Wang, Yisheng

doi:10.1063/1.1795111

Cited by 29 publications

(25 citation statements)

References 19 publications

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“…Isolation of a particular m/z inside the ion trap ensures that both the charge state of the sample is known and, importantly, that contaminants are removed from the ion population under investigation. Several laboratories have implemented fluorescence excitation and detection capabilities into trapping mass spectrometry set-ups in order to individually probe mass-selected populations of large ions formed by ESI or MALDI using laser-induced fluorescence emission [28][29][30][31][32][33][34][35] as well as fluorescence lifetime measurements [36][37][38][39][40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence lifetimes of rhodamine dyes in vacuo

Nagy

Talbot

Czar

et al. 2012

Journal of Photochemistry and Photobiology A: Chemistry

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Section: Introductionmentioning

confidence: 99%

Fluorescence lifetimes of rhodamine dyes in vacuo

Nagy

Talbot

Czar

et al. 2012

Journal of Photochemistry and Photobiology A: Chemistry

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“…Recently, our group [1][2][3][4] and several others [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] have presented works focusing upon the development, characterization and applications of instrumentation for measuring laser-induced fluorescence from molecular ions in the gas phase. The majority of these studies have used highly fluorescent molecules such as the xanthene-based rhodamine dyes.…”

Section: Introductionmentioning

confidence: 99%

“…The instrumentation developed features laser light sources coupled into trapping mass spectrometers that are used to mass-select the ion population of interest and to store the selected ion population for extended periods of time. Several types of mass spectrometers have been employed, including 3-D Paul-type quadrupole ion traps (QIT) [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17], Fourier transform ion cyclotron resonance mass spectrometers (FT-ICR-MS) [18][19][20][21][22][23], and linear radiofrequency ion traps (LIT) [24]. Detection of integrated gas-phase fluorescence has been accomplished using high-sensitivity photomultiplier tubes [5-7, 9-11, 13-17, 21-24] or avalanche photodiodes [18,19,25], while dispersed fluorescence has been measured using spectrographs with charge-coupled device (CCD) detectors [1-4, 8, 12, 22].…”

Section: Introductionmentioning

confidence: 99%

Gas-Phase Fluorescence Excitation and Emission Spectroscopy of Three Xanthene Dyes (Rhodamine 575, Rhodamine 590 and Rhodamine 6G) in a Quadrupole Ion Trap Mass Spectrometer

Forbes

Jockusch

2011

J. Am. Soc. Mass Spectrom.

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The gas-phase fluorescence excitation, emission and photodissociation characteristics of three xanthene dyes (rhodamine 575, rhodamine 590, and rhodamine 6G) have been investigated in a quadrupole ion trap mass spectrometer. Measured gas-phase excitation and dispersed emission spectra are compared with solution-phase spectra and computations. The excitation and emission maxima for all three protonated dyes lie at higher energy in the gas phase than in solution. The measured Stokes shifts are significantly smaller for the isolated gaseous ions than the solvated ions. Laser power-dependence measurements indicate that absorption of multiple photons is required for photodissociation. Redshifts and broadening of the dispersed fluorescence spectra at high excitation laser power provide evidence of gradual heating of the ion population, pointing to a mechanism of sequential multiple-photon activation through absorption/emission cycling. The relative brightness in the gas phase follows the order R575 (1.00)GR590(1.15)GR6G(1.29). Fluorescence emission from several mass-selected product ions has been measured.

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“…In principle, optical spectroscopic investigations thereby can be combined with any of the mass spectrometric methods described previously [9,10]. Marshall et al have measured laser-induced fluorescence (LIF) excitation and emission spectra as well as LIF lifetimes of ions trapped in a Fourier transform ion cyclotron resonance (FTICR) cell [11][12][13]. Scott et al showed a dual cell setup for obtaining fluorescence lifetimes of trapped ions [14].…”

mentioning

confidence: 99%

Clear evidence of fluorescence resonance energy transfer in gas-phase ions

Dashtiev

Azov

Frankevich

et al. 2005

J. Am. Soc. Mass Spectrom.

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Fluorescence resonance energy transfer (FRET) is a distance-sensitive method that correlates changes in fluorescence intensity with conformational changes, for example, of biomolecules in the cellular environment. Applied to the gas phase in combination with Fourier transform ion cyclotron resonance mass spectrometry, it opens up possibilities to define structural/ conformational properties of molecular ions, in the absence of solvent, and without the need for purification of the sample. For successfully observing FRET in the gas phase it is important to find suitable fluorophores. In this study several fluorescent dyes were examined, and the correlation between solution-phase and gas-phase fluorescence data were studied. he study of biomolecular conformation in the gas phase has attracted great attention because it opens possibilities to compare gas-phase and solution-phase structures and to understand the effect of the solvent on a molecule. Because matrix-assisted laser desorption/ionization (MALDI) [1] and electrospray ionization (ESI) [2] typically generate unsolvated ions without any adducts, their structure also provides an ideal model system for theoretical calculations of conformations. A major question today is whether singly or multiply charged biomolecular ions produced by soft ionization methods retain their native (or at least a "native-like"), active conformation in the gas phase. This is often assumed but needs to be proven rigorously.Mass spectrometry has many attractive features for studying biomolecules in the gas phase, including the capability of the isolation of ions and elimination of unwanted species before detection and a positive identification of the molecule from the exact mass or from tandem mass spectrometry (MS/MS) data. There are mass spectrometric methods available for obtaining conformational information of molecules in the gas phase: blackbody infrared radiative dissociation where ions undergo unimolecular dissociation by exchanging their energy with the surroundings by absorption and emission of infrared photons [3], collision-induced dissociation where ions are dissociated as a result of interaction with a target neutral species [4], hydrogendeuterium exchange where hydrogen atoms of the protein are exchanged with the deuterium atoms from a solution [5], covalent or noncovalent tagging of biomolecules based on the surface accessibility of specific moieties [6,7], and ion mobility spectrometry where the measured cross section of the molecule is compared with a theoretically calculated cross section [8]. However, generally speaking, these methods yield only indirect information about the gas-phase conformation, which is often derived from either the overall cross section of the molecule, from a fragmentation pattern, or from a dissociation rate.Recently, the efficient trapping capabilities of mass spectrometry have been coupled with the high sensitivity of fluorescence detection to provide structural and spectroscopic information of molecules in the gas phase. The general idea...

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Time resolved laser-induced fluorescence of electrosprayed ions confined in a linear quadrupole trap

Cited by 29 publications

References 19 publications

Fluorescence lifetimes of rhodamine dyes in vacuo

Fluorescence lifetimes of rhodamine dyes in vacuo

Gas-Phase Fluorescence Excitation and Emission Spectroscopy of Three Xanthene Dyes (Rhodamine 575, Rhodamine 590 and Rhodamine 6G) in a Quadrupole Ion Trap Mass Spectrometer

Clear evidence of fluorescence resonance energy transfer in gas-phase ions

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