Time-lapse videography of human oocytes following intracytoplasmic sperm injection: Events up to the first cleavage division

Liu, Yanhe; Chapple, Vincent; Roberts, Peter; Ali, Jawad K.; Matson, Phillip

doi:10.1016/j.repbio.2014.08.003

Cited by 24 publications

(13 citation statements)

References 25 publications

(47 reference statements)

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“…However, no major delay seems to be present, since the MII rate was independent of age. The 2nd meiotic division may be influenced by age, since a recent publication comparing two age groups reports increased time to 2nd polar body extrusion after ICSI in women aged ≥38 years as compared to younger women [24]. This is in line with the effects of age on initial cleavages in the present study.…”

Section: Discussionsupporting

confidence: 89%

Effect of women’s age on embryo morphology, cleavage rate and competence—A multicenter cohort study

et al. 2017

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This multicenter cohort study on embryo assessment and outcome data from 11,744 IVF/ICSI cycles with 104,830 oocytes and 42,074 embryos, presents the effect of women’s age on oocyte, zygote, embryo morphology and cleavage parameters, as well as cycle outcome measures corrected for confounding factors as center, partner’s age and referral diagnosis. Cycle outcome data confirmed the well-known effect of women’s age. Oocyte nuclear maturation and proportion of 2 pro-nuclear (2PN) zygotes were not affected by age, while a significant increase in 3PN zygotes was observed in both IVF and ICSI (p<0.0001) with increasing age. Maternal age had no effect on cleavage parameters or on the morphology of the embryo day 2 post insemination. Interestingly, initial hCG value after single embryo transfer followed by ongoing pregnancy was increased with age in both IVF (p = 0.007) and ICSI (p = 0.001) cycles. For the first time, we show that a woman’s age does impose a significant footprint on early embryo morphological development (3PN). In addition, the developmentally competent embryos were associated with increased initial hCG values as the age of the women increased. Further studies are needed to elucidate, if this increase in initial hCG value with advancing maternal age is connected to the embryo or the uterus.

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Section: Discussionsupporting

confidence: 89%

Effect of women’s age on embryo morphology, cleavage rate and competence—A multicenter cohort study

et al. 2017

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“…In 2010, Wong and colleagues demonstrated that TLM could be used to predict blastocyst fate prior to embryonic genome activation (EGA) by measuring the duration of the first cytokinesis and the time between the second and third mitotic divisions (Wong et al 2010). Since this initial report, other studies have confirmed the importance of early cleavage divisions and identified additional imaging parameters predictive of developmental success (Cruz et al 2011; Meseguer et al 2011; Azzarello et al 2012; Cruz et al 2012; Dal Canto et al 2012; Hashimoto et al 2012; Hlinka et al 2012; Meseguer et al 2012; Rubio et al 2012; Desai et al 2014; Y. Liu et al 2014a, 2014b, 2015; Stensen et al 2015; Fig.…”

Section: Non-invasive Methods For Aneuploidy Detectionmentioning

confidence: 84%

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Daughtry

Chavez

2015

Cell Tissue Res

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Formation of a totipotent blastocyst capable of implantation is one of the first major milestones in early mammalian embryogenesis, but less than half of in vitro fertilized embryos from most mammals will progress to this stage of development. Whole chromosomal abnormalities, or aneuploidy, are key determinants of whether human embryos will arrest or reach the blastocyst stage. Depending on the type of chromosomal abnormality, however, certain embryos still form blastocysts and may be morphologically indistinguishable from chromosomally normal embryos. Despite the implementation of pre-implantation genetic screening and other advanced in vitro fertilization (IVF) techniques, the identification of aneuploid embryos remains complicated by high rates of mosaicism, atypical cell division, cellular fragmentation, sub-chromosomal instability, and micro-/multi-nucleation. Moreover, several of these processes occur in vivo following natural human conception, suggesting that they are not simply a consequence of culture conditions. Recent technological achievements in genetic, epigenetic, chromosomal, and non-invasive imaging have provided additional embryo assessment approaches, particularly at the single-cell level, and clinical trials investigating their efficacy are continuing to emerge. In this review, we summarize the potential mechanisms by which aneuploidy may arise, the various detection methods, and the technical advances (such as time-lapse imaging, “-omic” profiling, and next-generation sequencing) that have assisted in obtaining this data. We also discuss the possibility of aneuploidy resolution in embryos via various corrective mechanisms, including multi-polar divisions, fragment resorption, endoreduplication, and blastomere exclusion, and conclude by examining the potential implications of these findings for IVF success and human fecundity.

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“…In the present study we found that oocytes derived from the PCOS-IVM patients had significantly larger areas (mm 2 ) than those from the PCOS-ICSI and Control-ICSI groups at the earlier stages before cytokinesis. The ICSI procedure itself may influence the volume and size of the oocytes, and a decrease in the ellipse size of injected oocytes during the initial 2 hours after injection has been reported (20). However, because all three groups were inseminated using the ICSI technique, the variations in size between them are inherently controlled for.…”

Section: Discussionmentioning

confidence: 99%

Structural and morphologic differences in human oocytes after in vitro maturation compared with standard in vitro fertilization

Walls

Hart

Keelan

et al. 2016

Fertility and Sterility

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Time-lapse videography of human oocytes following intracytoplasmic sperm injection: Events up to the first cleavage division

Cited by 24 publications

References 25 publications

Effect of women’s age on embryo morphology, cleavage rate and competence—A multicenter cohort study

Effect of women’s age on embryo morphology, cleavage rate and competence—A multicenter cohort study

Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences

Structural and morphologic differences in human oocytes after in vitro maturation compared with standard in vitro fertilization

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