Time-lapse embryo imaging and morphokinetic profiling: Towards a general characterisation of embryogenesis

Mandawala, A.A.; Harvey, Simon C.; Roy, Tammie K.; Fowler, Katie E.

doi:10.1016/j.anireprosci.2016.09.015

Cited by 17 publications

(14 citation statements)

References 96 publications

(1 reference statement)

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“…Crucial to the temporal aspect of IDyOT developed below, time itself is also a modulatory factor for attentional orientation. Coull ( 2004 , p. 217) distinguishes between temporal attentional orienting (‘how attentional processing varies as a function of time’) and temporal selective attention (‘how time perception varies as a function of attentional selectivity’).…”

Section: Rhythm and Timing In Sequential Perceptionmentioning

confidence: 99%

“…Although the actual mechanism is the subject of much debate (for an overview see Hass and Durstewitz, 2014 ), we assume a neuronal representation of the passing of time to be available in the brain. moments in time can be related with respect to this underlying clock, and that the neural encoding forms the basis for the estimation of time intervals, which may be related to activation in brain areas such as the pre-supplementary motor area and frontal operculum (Coull, 2004 ).…”

Section: Idyot: the Information Dynamics Of Thinkingmentioning

confidence: 99%

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Entraining IDyOT: Timing in the Information Dynamics of Thinking

et al. 2016

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We present a novel hypothetical account of entrainment in music and language, in context of the Information Dynamics of Thinking model, IDyOT. The extended model affords an alternative view of entrainment, and its companion term, pulse, from earlier accounts. The model is based on hierarchical, statistical prediction, modeling expectations of both what an event will be and when it will happen. As such, it constitutes a kind of predictive coding, with a particular novel hypothetical implementation. Here, we focus on the model's mechanism for predicting when a perceptual event will happen, given an existing sequence of past events, which may be musical or linguistic. We propose a range of tests to validate or falsify the model, at various different levels of abstraction, and argue that computational modeling in general, and this model in particular, can offer a means of providing limited but useful evidence for evolutionary hypotheses.

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Section: Rhythm and Timing In Sequential Perceptionmentioning

confidence: 99%

Section: Idyot: the Information Dynamics Of Thinkingmentioning

confidence: 99%

Entraining IDyOT: Timing in the Information Dynamics of Thinking

et al. 2016

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“…TLM allows recording of the exact timing of each stage of embryo development (Cruz et al, ; Meseguer et al, ), and the analysis of acquired images allows identification of abnormal developmental patterns such as abnormal syngamy and abrupt direct (e.g., division from one blastomere directly to three or four blastomeres), reverse (apparent fusion of blastomeres), absent, and chaotic (division of one blastomere asynchronously with others) cleavage (Athayde Wirka et al, ; Cruz et al, ; Hojnik, Vlaisavljević, & Kovačič, ). Currently, TLM is used in human ART to select embryos for transfer (Castellò et al, ; Goodman et al, ), and there is a growing clinical interest in using it in livestock ART (Mandawala, Harvey, Roy, & Fowler, ; Park et al, ; Sugimura, Akai, & Imai, ). To the best of our knowledge, no studies have yet been reported on use of TLM to evaluate reproductive toxins in large animal models.…”

Section: Introductionmentioning

confidence: 99%

Altered morphokinetics in equine embryos from oocytes exposed to DEHP during IVM

Marzano

Mastrorocco

Zianni

et al. 2019

Molecular Reproduction Devel

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Di‐(2‐ethylhexyl) phthalate (DEHP) is a commonly used plasticizer with endocrine‐disrupting properties. In this study, we used an equine model to investigate DEHP concentrations in ovarian follicular fluid (FF), and to determine the effects of exposure of oocytes to potentially toxic concentrations of DEHP during in vitro maturation (IVM) on embryo development and quality. Embryo development was evaluated using time‐lapse monitoring (TLM), a photomicroscopic tool that reveals abnormalities in cleavage kinetics unobservable by conventional morphology assessment. Blastocyst bioenergetic/oxidative status was assessed by confocal analysis. The possibility that verbascoside (VB), a bioactive polyphenol with antioxidant activity, could counteract DEHP‐induced oocyte oxidative damage, was investigated. DEHP was detected in FF and in IVM media at concentrations up to 60 nM. Culture of oocytes in the presence of 500 nM DEHP delayed second polar body extrusion, reduced duration of the second cell cycle, and increased the percentage of embryos showing abrupt multiple cleavage, compared with controls. Mitochondrial activity and intracellular levels of reactive oxygen species were reduced in blastocysts from DEHP‐exposed oocytes. VB addition during IVM limited DEHP‐induced blastocyst damage. In conclusion, DEHP is detectable in equine FF and culture medium, and oocyte exposure to increased concentrations of DEHP during IVM affects preimplantation embryo development. Moreover, TLM, reported for the first time in the horse in this study, is an efficient tool for identifying altered morphokinetic parameters and cleavage abnormalities associated with exposure to toxic compounds.

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“…The evaluation of fertilization at a single time point, such as by observation of patterns of nucleolar precursor bodies (NPBs) or the presence of halos in cytoplasm, may predict IVF treatment outcome, but the effect is not always as expected [11,12]. Time-lapse embryo imaging enables non-invasive observation and setting of evaluation markers such as polar body extrusion, pronuclear formation, and cleavage times and patterns, as well as enabling identification of embryo quality and prediction of the pregnancy/implantation rate [13,14]. In-depth study of the fertilization process by time-lapse imaging shows a difference between normal and abnormal embryos in the mean duration of pronuclei fading (PNF) [15,16].…”

Section: Discussionmentioning

confidence: 99%

Does polycystic ovary syndrome affect morphokinetics or abnormalities in early embryonic development?

Lê

Nguyen

et al. 2019

European Journal of Obstetrics & Gynecology and Reproductiv

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Objectives This study aimed to investigate whether oocyte retrieval from PCOS patients affects the morphokinetics or the incidence of abnormalities in early embryonic development, using time-lapse imaging analysis. Methods This was a prospective study in total of 39 PCOS patients and 67 women with normal ovarian function, underwent a GnRH antagonist protocol of controlled ovarian stimulation and fertilization by ICSI. 402 zygotes from the PCOS group and 449 zygotes from the control group were observed by time-lapse monitoring for 48 h following sperm injection. Results Patients with PCOS showed a significantly higher number of retrieved oocytes, and a significantly higher number of metaphase II oocytes per cycle than that of the non-PCOS group (18.85 ± 9.41 vs. 11.48 ± 5.51 and 14.97 ± 7.43 vs. 9.51 ± 4.7, respectively). However, oocyte maturation rate and morphologically assessed embryo quality did not differ between two groups. After 48 h of the embryo culture using time-lapse video recording, most of the embryos in both groups had reached the 4-cell stage (353/449 vs. 314/402 embryos). There were no significant differences between the control and PCOS group regarding the time-points of the successive events in early embryonic development from the appearance of 2 polar bodies to the 6-cell stage. However, the percentage of t2 stages which fell in “optimal range” (>24 h and ≤28 h) was significantly lower in the PCOS group than in the control group (37.8% vs. 48.1%, P = 0.004). The proportion of embryos manifesting abnormal fertility, multinucleation, direct cleavage and reverse cleavage were not significantly different between the two groups. Conclusion Our data indicated that PCOS does not affect the morphokinetics or incidence of abnormalities in early embryonic development.

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Time-lapse embryo imaging and morphokinetic profiling: Towards a general characterisation of embryogenesis

Abstract: 20In vitro fertilisation is an effective method of assisted reproductive technology in both humans and 21 certain non-human animal species. In most species, specifically, in humans and livestock, high in vitro

Cited by 17 publications

References 96 publications

Entraining IDyOT: Timing in the Information Dynamics of Thinking

Entraining IDyOT: Timing in the Information Dynamics of Thinking

Altered morphokinetics in equine embryos from oocytes exposed to DEHP during IVM

Does polycystic ovary syndrome affect morphokinetics or abnormalities in early embryonic development?

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