TIME-EFFICIENT pH-BASED METHOD TO QUANTIFY THE ACTIVITY OF ALKALINE AND HALO-ALKALINE PROTEASES

Abstract

Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)

Characterization of Geobacillus stearothermophilus protease for detergent industry

“…Alkaline protease with high temperature and alkaline pH optima is also desirable in detergents industry (Hang-seung and Jan, 2012). In a study protease produced from Bacillus safensis have shown optimal activity at at pH 8.0 and at 40°C and serine protease from Bacillus licheniformis showed stability at pH 7.0 (Ji et al, 2019;Shakir et al, 2019;Martínez-Pérez, 2019). In another study protease enzyme from Bacillus sp.…”

Biosynthesis of fibrinolytic agent urokinase by Enterococcus gallinarum isolated from sardine

“…After 10min of incubation at room temperature, absorbance was recorded at 660nm using a spectrophotometer (Model: 7200 Aquarius, Cecil CE, United Kingdom). One casein degradation unit calculated as the amount of enzyme that degraded casein and release 1 µmol of tyrosine under given assay conditions (Martínez-Pérez et al, 2019).…”