Time-dependent study of graphene oxide-trypsin adsorption interface and visualization of nano-protein corona

Kumari, Sujata; Sharma, Pratibha; Ghosh, Dibakar; Shandilya, Manish; Rawat, Pooja; Hassan, Md. Imtaiyaz; Moulick, Ranjita Ghosh; Bhattacharya, Jaydeep; Srivastava, Chandramohan; Majumder, Sudip

doi:10.1016/j.ijbiomac.2020.09.099

Cited by 12 publications

(8 citation statements)

References 61 publications

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“…Also, there is a red shift on the interaction with protein, suggesting opening of the β-sheet structure of protein. Trypsin has a characteristic signal at 336 nm, but when it interacts with GO, the signal shifts to 342 nm, indicating that the sheet structure opens up and quenching occurs …”

Section: Protein/dna Interaction With Graphene Oxidementioning

confidence: 98%

“…Trypsin has a characteristic signal at 336 nm, but when it interacts with GO, the signal shifts to 342 nm, indicating that the sheet structure opens up and quenching occurs. 298 17.4. Graphene Oxide−Human Serum Albumin (HSA).…”

Section: Protein/dna Interaction With Graphene Oxidementioning

confidence: 99%

“… Fluorescence spectra of 1:1 Trp–GO at different time intervals. Reproduced with permission from ref ( 315 ). Copyright@2020, Elsevier, Ltd., International Journal of Biological Macromolecules.…”

Section: Protein/dna Interaction With Graphene Oxidementioning

confidence: 99%

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An Update on Graphene Oxide: Applications and Toxicity

et al. 2022

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Graphene oxide (GO) has attracted much attention in the past few years because of its interesting and promising electrical, thermal, mechanical, and structural properties. These properties can be altered, as GO can be readily functionalized. Brodie synthesized the GO in 1859 by reacting graphite with KClO 3 in the presence of fuming HNO 3 ; the reaction took 3−4 days to complete at 333 K. Since then, various schemes have been developed to reduce the reaction time, increase the yield, and minimize the release of toxic byproducts (NO 2 and N 2 O 4 ). The modified Hummers method has been widely accepted to produce GO in bulk. Due to its versatile characteristics, GO has a wide range of applications in different fields like tissue engineering, photocatalysis, catalysis, and biomedical applications. Its porous structure is considered appropriate for tissue and organ regeneration. Various branches of tissue engineering are being extensively explored, such as bone, neural, dentistry, cartilage, and skin tissue engineering. The band gap of GO can be easily tuned, and therefore it has a wide range of photocatalytic applications as well: the degradation of organic contaminants, hydrogen generation, and CO 2 reduction, etc. GO could be a potential nanocarrier in drug delivery systems, gene delivery, biological sensing, and antibacterial nanocomposites due to its large surface area and high density, as it is highly functionalized with oxygen-containing functional groups. GO or its composites are found to be toxic to various biological species and as also discussed in this review. It has been observed that superoxide dismutase (SOD) and reactive oxygen species (ROS) levels gradually increase over a period after GO is introduced in the biological systems. Hence, GO at specific concentrations is toxic for various species like earthworms, Chironomus riparius, Zebrafish, etc.

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Section: Protein/dna Interaction With Graphene Oxidementioning

confidence: 98%

Section: Protein/dna Interaction With Graphene Oxidementioning

confidence: 99%

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An Update on Graphene Oxide: Applications and Toxicity

et al. 2022

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“…GO tends to aggregate in the physiological environment as a result of the charge shielding effect of the surface groups . GO has a strong adsorption effect on proteins and is easily recognized and engulfed by macrophages in vivo , thereby causing inflammation . In addition, GBNs do not have targeted, slow, and controlled release functions in vivo .…”

Section: Functional Modificationmentioning

confidence: 99%

“…18 GO has a strong adsorption effect on proteins and is easily recognized and engulfed by macrophages in vivo, thereby causing inflammation. 58 In addition, GBNs do not have targeted, slow, and controlled release functions in vivo. Collectively, these shortcomings limit the application of GBNs in biomedicine.…”

Section: Functional Modificationmentioning

confidence: 99%

Promising Graphene-Based Nanomaterials and Their Biomedical Applications and Potential Risks: A Comprehensive Review

Zeng

et al. 2021

ACS Biomater. Sci. Eng.

100

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Graphene-based nanomaterials (GBNs) have been the subject of research focus in the scientific community because of their excellent physical, chemical, electrical, mechanical, thermal, and optical properties. Several studies have been conducted on GBNs, and they have provided a detailed review and summary of various applications. However, comprehensive comments on biomedical applications and potential risks and strategies to reduce toxicity are limited. In this review, we systematically summarized the following aspects of GBNs in order to fill the gaps: (1) the history, synthesis methods, structural characteristics, and surface modification; (2) the latest advances in biomedical applications (including drug/gene delivery, biosensors, bioimaging, tissue engineering, phototherapy, and antibacterial activity); and (3) biocompatibility, potential risks (toxicity in vivo/vitro and effects on human health and the environment), and strategies to reduce toxicity. Moreover, we have analyzed the challenges to be overcome in order to enhance application of GBNs in the biomedical field.

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Exploring Nano‐Protein Corona Dynamics: Tracing the Hard‐to‐Soft Corona Transition with Trypsin and Graphene Oxide in a Silver Nanocomposite Model

Sharma,

Yadav,

Kumari

et al. 2024

ChemistrySelect

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In this work, the adsorption of Trypsin on synthesized Silver (Ag), Graphene Oxide: Silver (GO: Ag) nanocomposite (2:1), and Graphene Oxide (GO) nanoparticles (NPs) and, their effect on the conformation of Trypsin was studied by various spectroscopic and microscopic techniques to understand the transition of nano Protein Corona (PC) from “hard” to “soft.” The results showed that the adsorption of Trypsin on synthesized NPs followed the Freundlich adsorption isotherm and pseudo‐second‐order kinetics. The conformational changes that occurred in Trypsin upon interaction with synthesized NPs were investigated by using Circular Dichroism (CD), Fluorescence, and Fourier Transform Infrared (FTIR) spectroscopy. The morphological investigation of nano PC using High‐Resolution Transmission Electron Microscopy (HR‐TEM) and Atomic Force Microscopy (AFM) indicated the formation of hard, semi‐soft, and soft nano PC in the presence of Ag, GO: Ag, and GO NPs, respectively. The negative value of the thermodynamic parameters ΔG, ΔH, and ΔS in all three cases suggests the reaction to be exothermic and spontaneous. The van der Waals interaction and hydrogen bonding are the major forces responsible for binding Trypsin on the surface of Ag, GO: Ag, and GO. The catalytic efficiency of trypsin in the absence and presence of NPs was examined by performing the protein assay showing the highest catalytic activity of pure Trypsin compared with trypsin‐NPs constructs. Our findings provide useful information for the applications of GO‐based nanocomposites for various biological applications.

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Time-dependent study of graphene oxide-trypsin adsorption interface and visualization of nano-protein corona

Cited by 12 publications

References 61 publications

An Update on Graphene Oxide: Applications and Toxicity

An Update on Graphene Oxide: Applications and Toxicity

Promising Graphene-Based Nanomaterials and Their Biomedical Applications and Potential Risks: A Comprehensive Review

Exploring Nano‐Protein Corona Dynamics: Tracing the Hard‐to‐Soft Corona Transition with Trypsin and Graphene Oxide in a Silver Nanocomposite Model

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