Time-Dependent Proteomic iTRAQ Analysis of Nasal Lavage of Hairdressers Challenged by Persulfate

Abstract

Hairdressers are frequently exposed to bleaching powder containing persulfates, a group of compounds that may induce hypersensitivity in the airways. The mechanism causing this reaction is not clear. The aim of this study was to identify changes in the nasal lavage fluid proteome after challenge with potassium persulfate in hairdressers with bleaching powder-associated rhinitis. Furthermore, we aimed to compare their response to that of hairdressers without nasal symptoms, and atopic subjects with pollen-associated nasal symptoms. To study the pathogenesis of persulfate-associated rhinitis, the response in protein expression from the upper airway was assessed by time-dependent proteomic expression analysis of nasal lavage fluids. Samples were prepared by pooling nasal lavage fluids from the groups at different time points after challenge. Samples were depleted of high-abundant proteins, labeled with iTRAQ and analyzed by online 2D-nanoLC-MS/MS. Differences in the protein pattern between the three groups were observed. Most proteins with differentially expressed levels were involved in pathways of lipid transportation and antimicrobial activities. The major finding was increased abundance of apolipoprotein A-1, 20 min postchallenge, detected solely in the group of symptomatic hairdressers. Our results suggest there may be differences between the mechanisms responsible for the rhinitis in the symptomatic and atopic group.

High-Density Lipoprotein (HDL) in Allergy and Skin Diseases: Focus on Immunomodulating Functions

“…Other studies reported an increase in apoA-I and apoA-II, which are the major HDL apolipoproteins, in the mucus proteome of allergic rhinitis patients, suggesting a direct modulation of the immune response by HDL apolipoproteins [127][128][129]. Increased apoA-I levels were also detected in nasal lavage fluids of subjects with persulfate-associated rhinitis after challenge with potassium persulfate [130]. Moreover, in allergic rhinitis patients, correlations of specific IgE levels of grass pollen were observed with apoE and SAA content of HDL [29].…”

Role of Short Chain Fatty Acids and Apolipoproteins in the Regulation of Eosinophilia-Associated Diseases

“…A study from 2010 investigated changes in the nasal lavage fluid proteome after allergen challenge of potassium persulfate sensitized allergic rhinitis patients. As a major finding, increased abundance of apoA-I was detected post-challenge solely in the group of symptomatic patients [ 104 ]. In further proteome studies, apoA-I was significantly upregulated in the nasal mucus of allergic rhinitis patients, indicating a direct modulation of the local immune response by apoA-I [ 105 , 106 ].…”

Functionality of HDL: Antioxidation and Detoxifying Effects

“…Lipids are present in human tears (Rantamaki et al 2011), and apoA-I has been detected in nasal lavage fluid (NLF) (Ghafouri et al 2002) and saliva (Ghafouri et al 2003). Interestingly, in NLF, apoA-I was shown to increase in hairdressers after 20 min of exposure to persulphates (Karedal et al 2010), whilst apoA-I in gingival crevicular fluid has been suggested to be a novel periodontal disease marker (Tsuchida et al 2012). In addition, decreased levels of apoA-I have been reported in bronchio-alveolar lavage fluids of patients with idiopathic pulmonary fibrosis compared to healthy controls (Kim et al 2010).…”