Time-dependent effect of rutin on skin fibroblasts membrane disruption following UV radiation

Abstract

Chronic exposure of the skin to solar UV radiation induces a number of biological alterations, including a redox imbalance; therefore, there is an urgent need for skin cells protective compounds. The aim of this study was to determine the effects of natural, previously extensively examined, polyphenol with antioxidant properties – rutin, on UV-induced skin fibroblasts membrane disruption. Accordingly, fibroblasts exposed to UVA and UVB irradiation were incubated with rutin (12 h before and/or up to 24 h after irradiation), and the structural and metabolic changes were examined. Rutin penetration through the fibroblast phospholipid bilayer was aided by UVA-induced bilitranslocase activity 2–4 h after irradiation, while UVB irradiation led to enhanced phospholipid peroxidation and higher membrane permeability to facilitate the interaction of rutin with phospholipids. Lipidomic analysis revealed that 4 h of rutin treatment also partially prevented UVA/B-induced increase in phosphatidylethanolamine and phosphatidylcholine level, as well as their membrane localization, which resulted in an enhanced zeta potential in the cells and liposomes. Moreover, rutin 2 h following irradiation, in a various degree, prevented the increased in phospholipase A2 activity and ROS generation, and partially protected against the reduction of arachidonic and linoleic acids level and the lipid peroxidation product 4-hydroxynonenal level increase. Rutin effectively prevented against decrease in glutathione peroxidase, glutathione and vitamins E and C activities/levels, particularly 2 h following UVA irradiation.In conclusion, highest skin fibroblasts membrane level of rutin occurred in 2–4 h following UVA/B-radiation results in its strongest effect on biomembrane structure and functions and cellular antioxidant system irrespective of the radiation type.

The effect of Cyanidin-3-o-glucoside on UVA-induced damage in human dermal fibroblasts

“…UVA can damage human skin fibroblasts by inducing ROS . In particular, the cytotoxic activities of ROS lead to apoptosis .…”

“…UVA can damage human skin fibroblasts by inducing ROS. 22 In particular, the cytotoxic activities of ROS lead to apoptosis. 23 In our experiments, the cell survival rate was decreased by UVA ( Figure 2B), and HDFs showed increased ROS levels ( Figure 3A,B,D) as well as increased apoptosis (Figure 3C,E) when exposed to 12 J/cm 2 of UVA.…”

Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation

“…The bilitranslocase transport activity was measured spectrophotometrically [20,59]. The results were normalized to protein concentration and expressed as units per milligram of protein.…”

“…The rutin level was measured using an Agilent 1290 HPLC system [20]. The mobile phase consisted of water containing 0.1% (v/v) TFA with linear gradients of 5% acetonitrile at t = 0-20% acetonitrile at 5 min, followed by an increase to 30% acetonitrile at 10 min.…”

Ultraviolet A protective potential of plant extracts and phytochemicals

“…In another study on CCD 1112Sk human fibroblasts, RU application also counteracted ROS generation and prevented UVA-and UVB-induced changes in the composition of membrane phospholipids and free fatty acids content, the oxidation of phospholipids and biomembrane destruction, depletion of the GSH/GSSG ratio, GPx activity and levels of vitamins E and C (ref. 140 ). RU further reduced UVA-induced pro-inflammatory response and ROS generation, and enhanced the activity/ level of antioxidants (SOD, GPx, vitamin E, GSH, and Trx).…”