Time Course of the Biotransformation of Abscisic Acid and trans-Abscisic Acid Conjugates in Cell Suspension Cultures of Lycopersicon peruvianum

Lehmann, H.

doi:10.1016/s0015-3796(83)80066-3

Cited by 6 publications

(6 citation statements)

References 8 publications

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“…These findings are in contrast with most reports, where the principal compound found has been cis-trans ABA rather than transtrans ABA, However, some authors found traces (Goldschmidt et al, 1973, Rivier andPilet 1981) and even high levels (10-fold greater than cis-trans ABA) of trans-trans ABA in dormant citrus buds (Jones et al 1976) and in apple fruits (Bangerth 1982). Trans-trans ABA is generally regarded as a biologically inactive derivative of cis-trans ABA (Milborrow 1974, Iversen et al, 1983, An outstanding point in the present study is that the highest levels of trans-trans ABA were found in bound forms, especially in cotyledonary buds (Fig, 2B), This finding is in good agreement with work of Lehmann and Vlasov (1982) on cell suspension cultures of Lycopersicon peruvianum. These authors demonstrated that the trans-trans ABA isomer did not stay in the free form, was quickly converted to the glucose conjugate and never gave acidic metabolites as opposed to the cis-trans form.…”

Section: Presence Of Trans-trans Abasupporting

confidence: 91%

“…To our knowledge, such a relation has never been established in the literature as a function of the degree of inhibition of buds. In the same way, the levels of glucosylated compounds rise and the free ABA decreases in apple seeds during the release from dormancy (Barthe and Bulard 1978), The conjugated metabolites have often been considered as deactivation forms of the biologically active hormone, and ABA conjugation generally seems irreversible (Milborrow 1978. Lehmann 1983; but they could also be reactivated and give increasing amounts of ABA as ABA-Glu decreases (Hoque et al, 1983),…”

Section: Bound Aba In the Budsmentioning

confidence: 99%

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Distribution of free and bound forms of cis‐trans and trans‐trans abscisic acid in broad‐bean plants in relation to apical dominance

Everat‐Bourbouloux

1987

Physiologia Plantarum

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Levels of endogenous abscisic acid (ABA; free and bound forms) have been determined by gas chromatography in stems and buds of broad‐bean plants (Vicia faba L. cv. Aguadulce) in relation to apical dominance. A downward gradient of free cis‐trans ABA occurred along the stem, from the apical bud to the roots. Except for the actively growing apical bud the levels of free cis‐trans ABA were higher in the buds than in the corresponding nodes. An inverse correlation can be set up between levels of free cis‐trans ABA and growth of buds, except for the cotyledonary ones. High levels of bound ABA (cis‐trans form) are correlated with the growth of the apical bud and that of the axillary bud ax1. The hormonal regulation of the growth of the cotyledonary buds, which contained high levels of trans‐trans ABA in bound forms, is apparently different from that of the other buds.

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Section: Presence Of Trans-trans Abasupporting

confidence: 91%

Section: Bound Aba In the Budsmentioning

confidence: 99%

Distribution of free and bound forms of cis‐trans and trans‐trans abscisic acid in broad‐bean plants in relation to apical dominance

Everat‐Bourbouloux

1987

Physiologia Plantarum

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“…The free acid appeared in the cells as well as in the medium after 20 min, but the conjugate was not detected in the cells until much later. It was suggested that hydrolysis took place at the cell membrane, the free acid then entered the cells, and some of it was conjugated again later (20). In conclusion, these observations indicate that ABA-GE cannot enter cells without first being hydrolyzed.…”

Section: Discussionmentioning

confidence: 74%

“…In Ricinus, application of [3H]ABA-GE to leaves resulted in hydrolysis prior to uptake of free ABA into the phloem (Fig. 4). ['4C]ABA-GE was also rapidly hydrolyzed by cell suspension cultures of Lycopersicon (20). The free acid appeared in the cells as well as in the medium after 20 min, but the conjugate was not detected in the cells until much later.…”

Section: Discussionmentioning

confidence: 98%

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Accumulation and Transport of Abscisic Acid and Its Metabolites in Ricinus and Xanthium

Zeevaart¹,

Boyer²

1984

Plant Physiol.

106

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When intact plants of Xanthium strumanium L. were water stressed, the youngest leaves accumulated the highest levels ofabscisic acid (ABA).On the other hand, when leaves of different ages were detached and then stressed, the capacity to produce ABA was highest in the mature leaves. Radioactive ABA was transported from mature leaves to the shoot tips and young leaves, as well as to the roots, as evidenced by the presence of radioactive ABA and phaseic acid in the xylem exudate coming from the roots. Thus, ABA was recirculated in the plant, moving down the stem in the phloem and back up in the transpiration stream to the mature leaves. Phloem exudate collected by the use of the EDTA technique had a high concentration of ABA and phaseic acid which increased severalfold after water stress. The high ABA levels in immature leaves and apical buds are, therefore, mainly due to import from older leaves, rather than to in situ synthesis.In Ricinus communis L. cv gibsonii labeled ABA was rapidly exported from a mature leaf as measured by the appearance of radioactive ABA in the phloem collected from incisions in the stem below the treated leaf. After 2 h, small amounts of radioactive phaseic acid and dihydrophaseic acid were also present in the phloem exudate. The glucosyl ester of abscisic acid, fl-D-glucopyranosyl abscisate, was hydrolyzed before entering the phloem and moved as free ABA. It is concluded that the glucosyl ester of ABA itself cannot cross cell membranes and is, therefore, not transported.The highest levels of ABA in vegetative tissues are found in immature leaves and buds (e.g. 14,22,24,25). This difference in ABA content could be the result of (a) a higher capacity for ABA synthesis in young leaves than in mature ones, or (b) translocation of ABA with the assimilates from mature source leaves to the young sink tissues. This latter possibility is a real one, since ABA is known to be present in sieve tube sap (e.g. 14, 16, 18, 25). The most direct approach to this question would be in vitro studies on the ABA-synthesizing capacity in extracts from leaves ofdifferent ages. Since such a system is not available at present, only indirect approaches can be used to study this problem.In this paper we report on the effects of water stress on the ABA levels in Xanthium leaves of different ages that were detached, or left on the plant. To demonstrate the movement of ABA from source to sink leaves, use was made of radioactive ABA. We further analyzed phloem and xylem exudates collected '

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Abscisic acid is present in liverworts

Wurtele²,

LaMotte

1994

Phytochemistry

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Time Course of the Biotransformation of Abscisic Acid and trans-Abscisic Acid Conjugates in Cell Suspension Cultures of Lycopersicon peruvianum

Cited by 6 publications

References 8 publications

Distribution of free and bound forms of cis‐trans and trans‐trans abscisic acid in broad‐bean plants in relation to apical dominance

Distribution of free and bound forms of cis‐trans and trans‐trans abscisic acid in broad‐bean plants in relation to apical dominance

Accumulation and Transport of Abscisic Acid and Its Metabolites in Ricinus and Xanthium

Abscisic acid is present in liverworts

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