Abscisic acid is present in liverworts

Li, Xiaoyue; Wurtele, Eve Syrkin; LaMotte, Clifford E.

doi:10.1016/s0031-9422(00)90328-5

Cited by 25 publications

(16 citation statements)

References 21 publications

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“…Our estimation by ELISA indicated that thalli of M. polymorpha cultured in normal medium for 4 weeks (Fig. 1A) contain 16.5 6 3.9 ng g 21 fresh weight endogenous ABA, comparable to the levels reported by Li et al (1994). Previous reports indicated that thalli of M. polymorpha show little ABA responses and desiccation stress tolerance (Hellwege et al, 1994;Pence et al, 2005).…”

Section: Discussionsupporting

confidence: 86%

“…Levels of endogenous ABA have also been estimated in R. fluitans using a similar method (Hellwege et al, 1992(Hellwege et al, , 1996. Li et al (1994) reported on the detection of ABA in M. polymorpha thalli by measurements using gas chromatography with mass spectrometry and estimated its endogenous levels to be 4 to 16 ng g 21 fresh weight, similar to those of higher plants. Our estimation by ELISA indicated that thalli of M. polymorpha cultured in normal medium for 4 weeks (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Despite the experimental facts that show increased stress tolerance induced by exogenous ABA application, the physiological role of ABA in liverworts has been underestimated because it has been believed that liverworts do not have endogenous ABA. However, Li et al (1994) reported that gametophytes of Marchantia polymorpha contain endogenous ABA at concentrations similar to those in higher plants. It was also reported that E. holstii and R. fluitans contain endogenous ABA (Hellwege et al, 1992(Hellwege et al, , 1994.…”

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confidence: 99%

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Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

et al. 2010

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Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the role of ABA has been least emphasized; thus, very little information is available on the molecular mechanisms underlying ABA responses. In this study, we isolated and characterized MpABI1, an ortholog of ABSCISIC ACID INSENSITIVE1 (ABI1), from the liverwort Marchantia polymorpha. The MpABI1 cDNA encoded a 568-amino acid protein consisting of the carboxy-terminal protein phosphatase 2C (PP2C) domain and a novel amino-terminal regulatory domain. The MpABI1 transcript was detected in the gametophyte, and its expression level was increased by exogenous ABA treatment in the gemma, whose growth was strongly inhibited by ABA. Experiments using green fluorescent protein fusion constructs indicated that MpABI1 was mainly localized in the nucleus and that its nuclear localization was directed by the aminoterminal domain. Transient overexpression of MpABI1 in M. polymorpha and Physcomitrella patens cells resulted in suppression of ABA-induced expression of the wheat Em promoter fused to the b-glucuronidase gene. Transgenic P. patens expressing MpABI1 and its mutant construct, MpABI1-d2, lacking the amino-terminal domain, had reduced freezing and osmotic stress tolerance, and associated with reduced accumulation of ABA-induced late embryogenesis abundant-like boiling-soluble proteins. Furthermore, ABA-induced morphological changes leading to brood cells were not prominent in these transgenic plants. These results suggest that MpABI1 is a negative regulator of ABA signaling, providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in liverworts.Land plants are repeatedly challenged by environmental stresses such as desiccation, salinity, and freezing, which can cause irreversible damage to intracellular structures and membranes by severe dehydration. Abscisic acid (ABA), which is known to be a phytohormone responsible for physiological control of seed maturation and germination, bud dormancy, and stomata closure, has been postulated to be involved in the development of cellular dehydration tolerance under stress conditions. In higher plants, water-stress conditions trigger a transient or sustained increase in endogenous ABA content in tissues. Increased ABA stimulates the expression of a number of stress-related genes such as those of late embryogenesis abundant (LEA) proteins, which are thought to protect cells from the damage caused by the dehydration stress (Chandler and Robertson, 1994). It has been demonstrated that various tissue-cultured cells of vascular plants, including those of angiosperms, gymnosperms, and ferns, develop desiccation and freezing tolerance upon exogenous ABA treatment, indicating that ABA plays a key role in stress tolerance at cellular

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

et al. 2010

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“…b State diagrams of glass transition for M. polymorpha cultured cells with preculture. The cells were desiccated on silica gel and then equilibrated at 12-76% RH other hand, exogenous ABA did not affect survival after drying in tissue of M. polymorpha (Pence et al 2005), even though ABA has been reported to be present in vegetative thalli of this plant (Li et al 1994). Our experiment also showed that ABA treatment of cultured cells of M. polymorpha could not induce a high level of desiccation tolerance but did induce some tolerance as well as in intact tissues (Fig.…”

Section: Discussionmentioning

confidence: 46%

Development of desiccation tolerance and vitrification by preculture treatment in suspension-cultured cells of the liverwort Marchantia polymorpha

Hatanaka

Sugawara

2010

Planta

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Some cultured plant cells are able to acquire tolerance to various stresses when they are cultured under suitably controlled conditions. Induction of a high level of desiccation tolerance in suspension-cultured cells of the liverwort Marchantia polymorpha was examined for studying the mechanisms of desiccation tolerance and vitrification at the cellular level. Desiccation tolerance level of cells was very low and the survival rate was less than 10% after exposure to drying below 0.1 g H(2)O g(-1) dry weight (DW). Preculture treatment in 0.5 M sucrose medium was the most effective method for inducing a high level of desiccation tolerance in cells and the survival rate was 87% even after being desiccated to below 0.1 g H(2)O g(-1) DW. Preculture treatment caused alteration of cell structures and accumulation of a large amount of sucrose and newly synthesized proteins in cells. Abundant sucrose and preculture-induced proteins were necessary for full development of desiccation tolerance in the cells. When water content decreased to below 0.1 g H(2)O g(-1) DW, desiccation-tolerant cells that had been precultured were vitrified above 0 degrees C and maintained stable viability. We have succeeded in the induction of desiccation tolerance that allows formation of intracellular glass with cell viability at ambient temperatures by controlling culture conditions, and our results suggest that suspension-cultured cells of M. polymorpha are useful for studying cellular mechanisms for the development of desiccation tolerance and the stabilization of vitrified cells.

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“…A potential problem for these measurements was cross-reactivity of the antibodies against ABA with LNA (Yoshikawa et al 2002). However, Li et al (1994) conducted direct measurements of endogenous ABA in M. polymorpha and showed that its thalli contain 4-16 ng of ABA per gram fresh weight, comparable to the levels in angiosperms. These results in conjunction with the effect of exogenous ABA on inhibition of growth and development of stress tolerance suggested that ABA act as a phytohormone in liverworts.…”

Section: Bryophytes and Abamentioning

confidence: 99%

ABA in bryophytes: how a universal growth regulator in life became a plant hormone?

2011

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Abscisic acid (ABA) is not a plant-specific compound but one found in organisms across kingdoms from bacteria to animals, suggesting that it is a ubiquitous and versatile substance that can modulate physiological functions of various organisms. Recent studies have shown that plants developed an elegant system for ABA sensing and early signal transduction mechanisms to modulate responses to environmental stresses for survival in terrestrial conditions. ABA-induced increase in stress tolerance has been reported not only in vascular plants but also in non-vascular bryophytes. Since bryophytes are the key group of organisms in the context of plant evolution, clarification of their ABA-dependent processes is important for understanding evolutionary adaptation of land plants. Molecular approaches using Physcomitrella patens have revealed that ABA plays a role in dehydration stress tolerance in mosses, which comprise a major group of bryophytes. Furthermore, we recently reported that signaling machinery for ABA responses is also conserved in liverworts, representing the most basal members of extant land plant lineage. Conservation of the mechanism for ABA sensing and responses in angiosperms and basal land plants suggests that acquisition of this mechanism for stress tolerance in vegetative tissues was one of the critical evolutionary events for adaptation to the land. This review describes the role of ABA in basal land plants as well as non-land plant organisms and further elaborates on recent progress in molecular studies of model bryophytes by comparative and functional genomic approaches.

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Abscisic acid is present in liverworts

Cited by 25 publications

References 21 publications

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

Development of desiccation tolerance and vitrification by preculture treatment in suspension-cultured cells of the liverwort Marchantia polymorpha

ABA in bryophytes: how a universal growth regulator in life became a plant hormone?

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