Time Course of Influence by Ovariectomy and Calcium Diet on Bone Properties in Mice.

Minematsu, Akira; Yoshimura, Osamu; Yotsuji, Hirofumi; Ichigo, Hirozo; Kobayashi, Ryuji; Sasaki, Hisato; Tanaka, Sachiko; Kanemura, Naohiko; Shirahama, Kunji; Miyamoto, Hisakazu

doi:10.1298/jjpta.4.19

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…This decreased density was consistent with other short-term OVX studies ( Fanti et al, 1998 ) and demonstrates that 17α-E2 is able to induce beneficial effects on diaphyseal bone. We also found that OVX resulted in greater femur length, which is commonly observed following OVX due to endogenous estrogens modulating osteoblast activity at the growth plate ( Minematsu et al, 2001 ). Importantly, 17α-E2 prevented increases in femur length, which suggests that 17α-E2 may be acting through ERα similarly to 17β-E2 to regulate growth plate closure ( Borjesson et al, 2012 ); a possibility that will need to be explored through future studies.…”

Section: Discussionsupporting

confidence: 51%

17α-Estradiol prevents ovariectomy-mediated obesity and bone loss

Mann

Pitel

Nelson-Holte

et al. 2020

Experimental Gerontology

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Menopause is a natural physiological process in older women that is associated with reduced estrogen production and results in increased risk for obesity, diabetes, and osteoporosis. 17α-estradiol (17α-E2) treatment in males, but not females, reverses several metabolic conditions associated with advancing age, highlighting sexually dimorphic actions on age-related pathologies. In this study we sought to determine if 17α-E2 could prevent ovariectomy (OVX)-mediated detriments on adiposity and bone parameters in females. Eight-week-old female C57BL/6J mice were subjected to SHAM or OVX surgery and received dietary 17α-E2 during a six-week intervention period. We observed that 17α-E2 prevented OVX-induced increases in body weight and adiposity. Similarly, uterine weight and luminal cell thickness were decreased by OVX and prevented by 17α-E2 treatment. Interestingly, 17α-E2 prevented OVX-induced declines in tibial metaphysis cancellous bone. And similarly, 17α-E2 improved bone density parameters in both tibia and femur cancellous bone, primarily in OVX mice. In contrast, to the effects on cancellous bone, cortical bone parameters were largely unaffected by OVX or 17α-E2. In the non-weight bearing lumbar vertebrae, OVX reduced trabecular thickness but not spacing, while 17α-E2 increased trabecular thickness and reduced spacing. Despite this, 17α-E2 did improve bone volume/tissue volume in lumbar vertebrae. Overall, we found that 17α-E2 prevented OVX-induced increases in adiposity and changes in bone mass and architecture, with minimal effects in SHAM-operated mice. We also observed that 17α-E2 rescued uterine tissue mass and lining morphology to control levels without inducing hypertrophy, suggesting that 17α-E2 could be considered as an adjunct to traditional hormone replacement therapies.

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Section: Discussionsupporting

confidence: 51%

17α-Estradiol prevents ovariectomy-mediated obesity and bone loss

Mann

Pitel

Nelson-Holte

et al. 2020

Experimental Gerontology

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“…[ 22 ] Another study found that the ash weight of the femur in mice was 58% of the dry weight. [ 23 ] DXA has the advantage of being able to simultaneously do both bone-related measurements and quantitative analysis of soft tissues, such as fat and lean. In addition, since X-rays used in DXA react with all substances and are attenuated, weight can be accurately calculated, and image analysis information on the shape or fat distribution of bones and organs is also provided through measurement images.…”

Section: Discussionmentioning

confidence: 99%

Validation of Dual Energy X-Ray Absorptiometry and Nuclear Magnetic Resonance in the Analysis of Body Composition in Mice

et al. 2020

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Background As an instrument for measuring body composition in experimental animals, dual energy X-ray absorptiometry (DXA) is ideal for accuracy, cost, and measurement efficiency. However, there is too little insight into the effectiveness of the various aspects of applying DXA to experimental animals. We investigated whether to compare and verify the precision and accuracy of DXA and nuclear magnetic resonance (NMR) animal body composition analyzers. Methods We used 30 Institution of Cancer Research mice in the study. First, in order to evaluate the reproducibility of DXA and NMR, we did repeated measurements by repositioning each mouse in anesthesia and euthanasia states. Subsequently, the accuracy of each device was evaluated by comparing the weight measured before the experiment, the weight of the tissue extracted from the mice after the experiment, and the measured DXA and NMR. In addition, when measuring the body composition of animals, we compared the time and the measurable body composition parameters and summarized the advantages and disadvantages of the 2 devices. Results Compared to NMR, DXA had the advantage of a fast measurement of bone composition and rapid image analysis. In addition, DXA showed a higher correlation (>95%) with fat mass, lean mass baseline than did NMR (>85%). Conclusions In conclusion, DXA was confirmed to have higher precision and measurement accuracy than did NMR. Therefore, DXA is an effective method for evaluating the body composition of experimental animals.

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“…In another study, the dried and ash weights were 67% and 32% of the fresh weight of the right tibia and femur of hens [16]. Another study found that the ash weight of the femur in mice was 58% of the dry weight [17]. DXA has the advantage of being able to simultaneously do both bone-related measurements and quantitative analysis of soft tissues, such as fat and lean.…”

Section: Discussionmentioning

confidence: 99%

Verification of precision, accuracy, and characteristics of dual-energy X-ray absorptiometry and nuclear magnetic resonance in the analysis of mouse body composition

Baek¹,

Kim²,

Park³

et al. 2020

Preprint

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Background: As an instrument for measuring body composition in experimental animals, dual-energy X-ray absorptiometry (DXA) is ideal for accuracy, cost, and measurement efficiency. However, there is too little insight into the effectiveness of the various aspects of applying DXA to experimental animals. Therefore, we investigated whether to compare and verify the precision and accuracy of DXA and nuclear magnetic resonance (NMR) animal body composition analyzers. We used 30 ICR mice in the study. First, in order to evaluate the reproducibility of DXA and NMR, we did repeated measurements by repositioning each mouse in anesthesia and euthanasia states. Subsequently, the accuracy of each device was evaluated by comparing the weight measured before the experiment, the weight of the tissue extracted from the mice after the experiment, and the measured DXA and NMR. In addition, when measuring the body composition of animals, we compared the time and the measurable body composition parameters and summarized the advantages and disadvantages of the two devices.Results: Compared to NMR, DXA had the advantage of a fast measurement of bone composition and rapid image analysis. In addition, DXA showed a higher correlation (> 95%) with FM, body weight, and fBMC baseline than did NMR (> 85%).Conclusion: In conclusion, DXA was confirmed to have higher precision and measurement accuracy than did NMR. Therefore, DXA is an effective method for evaluating the body composition of experimental animals.

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Time Course of Influence by Ovariectomy and Calcium Diet on Bone Properties in Mice.

Cited by 4 publications

References 20 publications

17α-Estradiol prevents ovariectomy-mediated obesity and bone loss

17α-Estradiol prevents ovariectomy-mediated obesity and bone loss

Validation of Dual Energy X-Ray Absorptiometry and Nuclear Magnetic Resonance in the Analysis of Body Composition in Mice

Verification of precision, accuracy, and characteristics of dual-energy X-ray absorptiometry and nuclear magnetic resonance in the analysis of mouse body composition

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