Time course of changes in the intestinal permeability of food‐sensitized rats after oral allergen challenge

Abstract

Rats were sensitized with ovalbumin (OVA) with a molecular weight of 45 kd, challenged with OVA orally, followed by orally administered beta-lactoglobulin (BLG) as an intestinal permeability marker. BLG is a macro-molecular protein with a molecular weight of 18 kd. Blood BLG concentrations were measured (by ELISA) serially over 4 hours following BLG administration, which in turn was given 1 hour after OVA challenges. The maximum BLG concentration was at 2 hours. BLG was then administered orally 1, 3, 6, 12 and 24 hours after oral OVA challenge, and the serum BLG concentration at 2 hours after BLG administration was compared among the five groups. BLG appeared in the circulation of the animals 1, 6 and 24 hours after allergen challenge, but not after 3 and 12 hours. The serum BLG concentration was not significantly different at 1, 6 and 24 hours. Histopathological examinations of the intestines showed mast cell infiltration of the intestinal mucosa at 1 hour, remarkable edema of villi at 3 hours, eosinophil infiltration at 6 hours, an increase of goblet cells at 12 hours and villous atrophy and lymphocyte infiltration at 24 hours. The appearance in the serum of three BLG peaks of comparable heights suggested that the intestinal absorption of BLG may be related to a late and delayed phase as well as the immediate IgE-dependent phase response.

Antigen inhalation induces mast cells and eosinophils infiltration in the guinea pig esophageal epithelium involving histamine-mediated pathway

“…This is a typical late phase allergic response and the results are consistent with other studies on the airway hypersensitivity response (Dunn et al, 1988;Underwood et al, 1995), and intestinal food allergy response (Sakamoto et al, 1998). Pretreatment with thioperamide did not inhibit this increase in total number suggesting that the histamine H4 receptor regulates inflammatory cells infiltration only locally at the inflammation site but not in the circulation.…”

Mucosal Pathophysiology and Inflammatory Changes in the Late Phase of the Intestinal Allergic Reaction in the Rat

“…26 Wershil and colleagues 7 reported neutrophil and mononuclear cell infiltration in the stomach of mice after antigen challenge, with neutrophils peaking at 2 hours and mononuclear cells rising several fold at 12 to 24 hours. In another study, mast cell influx was observed at 1 hour, villus edema at 3 hours, eosinophil infiltration at 6 hours, and villus atrophy associated with increased numbers of lymphocytes at 24 hours, 27 although exact counts were not performed. Increased permeability was documented at 1, 6, and 24 hours with decreases between the peaks.…”

Soybean: feed quality and safety. Part 2: Pathology of soybean feeding in animals. A review

“…They demonstrated that removal of trypsin inhibitor did not result in normal pancreas weight and accounted for the hyperplastic effect of unheated soybean only in 40%. In a rat model, Sakamoto et al (1998) studied the time course of intestinal changes after challenging ovalbuminsensitised rats. One 1 h after challenge mast cell infiltration occurred in the small intestinal mucosa, the villi became oedematous 2 h later, by the 6th h eosinophilic infiltration dominated the histological changes, at the 12th h goblet cell numbers increased, and 24 h after challenge villus atrophy and lymphocyte infiltration occurred.…”