Time-course evaluation and treatment of skin inflammatory immune response after ultraviolet B irradiation

Paz, Mariela L.; Ferrari, Alejandro; Weill, Federico S.; Leoni, Juliana; Maglio, Daniel Horacio González

doi:10.1016/j.cyto.2008.06.012

Cited by 35 publications

(42 citation statements)

References 27 publications

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“…They were sacrificed 24 h later and histological analysis and cytokine quantification were performed. Figure 6 shows the skin sections of one representative mouse from each group of animals, where it can be seen that there were no differences between immunized non irradiated groups and the corresponding irradiated ones, except for the known effects of UVB irradiation on the skin [36,39]. Only one group showed a slight difference with respects to the others: when irradiated, group 1 presented a slight inflammatory infiltrate not seen in the other groups.…”

Section: In Vivo Modelmentioning

confidence: 99%

“…They were irradiated on their back with one minimal erythema dose (200 mJ/cm 2 ) of UVB, as described before [36]. After irradiation, animals were kept in the same conditions for 24 h and they were subsequently sacrificed.…”

Section: In Vivo Modelmentioning

confidence: 99%

“…HaCaT cells were cultured in plastic dishes and subconfluent cells were irradiated with 50 mJ/cm 2 of UVB light as previously described [36,37] in order to obtain a high percentage of apoptotic cells. At least one dish was not exposed to UVB irradiation, but was handled in the same fashion as the irradiated cells, that is to say the culture medium was replaced by PBS before the irradiation.…”

Section: In Vitro Modelmentioning

confidence: 99%

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Two Approaches to the Study of a Controversial Relationship: Cutaneous Photosensitivity and Anti-Ro/SS-A Autoantibodies

Paz¹,

Maiten²,

Ferrari³

et al. 2014

OJRA

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Background: Autoantibodies (Aabs) are the hallmark of numerous systemic autoimmune pathologies (SAPs), for instance anti-Ro/SS-A Aabs are usually found in Systemic Lupus Erythematosus (SLE) and Sjögren's Syndrome. Cutaneous photosensitivity (CP) is found in most forms and subsets of LE and consists of a skin rash as a result of unusual reaction to sunlight. There are many theories which relate specifically the presence of circulating antiRo/SS-A Aabs with the CP phenomenon, though there are several studies which are in disagreement. Results: In this study we analyzed the relationship between CP and anti-Ro Aabs by means of two approaches. The first one included an in vitro model where we evaluated by flow cytometry the binding capacity of affinity-purified Aabs to autoantigens relocalized on apoptotic keratinocyte's surface. We found that there was no relationship between the binding capacity of serum from 10 selected patients or their corresponding purified anti-Ro52 and anti-Ro60 Aabs, and the presence or absence of CP, neither with the SAPs. The in vivo model consisted of Hairless SKH:1 mice which were induced to produce anti-murine Ro52 and/or Ro60 Aabs and were subsequently irradiated with UVB light. We evaluated the skin histology and also the epidermal production of TNF-α. We found no differences between the groups in neither of the parameters evaluated. Conclusions: These results agree with some studies on the role of the Aabs in CP, considering anti-Ro Aabs not as the only responsible for the manifestation; and disagree with many other authors, who believe in the strong association between these two events.

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Section: In Vivo Modelmentioning

confidence: 99%

Section: In Vivo Modelmentioning

confidence: 99%

Section: In Vitro Modelmentioning

confidence: 99%

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Two Approaches to the Study of a Controversial Relationship: Cutaneous Photosensitivity and Anti-Ro/SS-A Autoantibodies

Paz¹,

Maiten²,

Ferrari³

et al. 2014

OJRA

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“…Keratinocytes, the most abundant cells in the skin, are a cell type that is particularly exposed to natural UVr. This stimulus is sufficient to induce the production of many inflammation-related molecules by the keratinocytes -such as TNF-, IL-6 and COX-2 itself-among others (Paz et al, 2008). Finally, there are many chemical agents used as tumor promoters (Lim et al, 2011;Rundhaug et al, 2007), which are able to induce the expression of COX-2.…”

Section: Stimulus and Mechanisms Involved In Cox-2 Expressionmentioning

confidence: 99%

“…among others (Paz et al, 2008;Trompezinski et al, 2001). Some of these molecules are known to mediate an important outcome of UVB irradiation: immunosuppression.…”

mentioning

confidence: 99%

Cyclooxygenase-2 Overexpression in Non-Melanoma Skin Cancer: Molecular Pathways Involved as Targets for Prevention and Treatment

Maglio¹,

Paz²,

Maiten³

et al. 2011

Skin Cancers - Risk Factors, Prevention and Therapy

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Vitamin D Induces Cyclooxygenase 2 Dependent Prostaglandin E₂ Synthesis in HaCaT Keratinocytes

Ravid

Shenker

Buchner-Maman

et al. 2015

Journal Cellular Physiology

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The active metabolite of vitamin D calcitriol and its analogs are well-known for their anti-inflammatory action in the skin, while their main side effect associated with topical treatment of inflammatory disorders is irritant contact dermatitis. Prostaglandin E2 (PGE2 ) is pro-inflammatory at the onset of inflammation and anti-inflammatory at its resolution. We hypothesized that induction of PGE2 synthesis by calcitriol in epidermal keratinocytes may contribute both to its pro-inflammatory and anti-inflammatory effects on the skin. Treatment of human immortalized HaCaT keratinocytes with calcitriol (3-100 nM, 2-24 h) increased PGE2 production due to increased mRNA and protein expression of COX-2, but not to increase of COX-1 or release of arachidonic acid. The effect of calcitriol on COX-2 mRNA was observed also in primary human keratinocytes. The increase in COX-2 mRNA is associated with COX-2 transcript stabilization. Calcitriol exerts this effect by a rapid (2 h) and protein synthesis independent mode of action that is dependent on PKC and Src kinase activities. Treatment with a COX-2 inhibitor partially prevented the attenuation of the keratinocyte inflammatory response by calcitriol. We conclude that upregulation of COX-2 expression with the consequent increase in PGE2 synthesis may be one of the mechanisms explaining the Janus face of calcitriol as both a promoter and attenuator of cutaneous inflammation. J. Cell. Physiol. 231: 837-843, 2016. © 2015 Wiley Periodicals, Inc.

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Time-course evaluation and treatment of skin inflammatory immune response after ultraviolet B irradiation

Cited by 35 publications

References 27 publications

Two Approaches to the Study of a Controversial Relationship: Cutaneous Photosensitivity and Anti-Ro/SS-A Autoantibodies

Two Approaches to the Study of a Controversial Relationship: Cutaneous Photosensitivity and Anti-Ro/SS-A Autoantibodies

Cyclooxygenase-2 Overexpression in Non-Melanoma Skin Cancer: Molecular Pathways Involved as Targets for Prevention and Treatment

Vitamin D Induces Cyclooxygenase 2 Dependent Prostaglandin E₂ Synthesis in HaCaT Keratinocytes

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Time-course evaluation and treatment of skin inflammatory immune response after ultraviolet B irradiation

Cited by 35 publications

References 27 publications

Two Approaches to the Study of a Controversial Relationship: Cutaneous Photosensitivity and Anti-Ro/SS-A Autoantibodies

Two Approaches to the Study of a Controversial Relationship: Cutaneous Photosensitivity and Anti-Ro/SS-A Autoantibodies

Cyclooxygenase-2 Overexpression in Non-Melanoma Skin Cancer: Molecular Pathways Involved as Targets for Prevention and Treatment

Vitamin D Induces Cyclooxygenase 2 Dependent Prostaglandin E2 Synthesis in HaCaT Keratinocytes

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Vitamin D Induces Cyclooxygenase 2 Dependent Prostaglandin E₂ Synthesis in HaCaT Keratinocytes