Tilapia Hepcidin 2-3 Peptide Modulates Lipopolysaccharide-induced Cytokines and Inhibits Tumor Necrosis Factor-α through Cyclooxygenase-2 and Phosphodiesterase 4D

Rajanbabu, Venugopal; Pan, Chieh-Yu; Lee, Shang-Chun; Lin, Wei-Ju; Lin, Chia Feng; Li, Chung-Leung; Chen, Jyh-Yih

doi:10.1074/jbc.m110.137935

Cited by 42 publications

(26 citation statements)

References 45 publications

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“…Hepcidin is a multifunctional peptide that is also involved in immunomodulatory functions as an antimicrobial peptide [109,110]. Several authors have reported that the hepcidin levels are significantly increased in fish after bacterial challenges [111][112][113].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Dietary administration of the probiotic SpPdp11: Effects on the intestinal microbiota and immune-related gene expression of farmed Solea senegalensis treated with oxytetracycline

Tapia‐Paniagua

Vidal

Lobo

et al. 2015

Fish & Shellfish Immunology

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Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Dietary administration of the probiotic SpPdp11: Effects on the intestinal microbiota and immune-related gene expression of farmed Solea senegalensis treated with oxytetracycline

Tapia‐Paniagua

Vidal

Lobo

et al. 2015

Fish & Shellfish Immunology

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“…Recently, the fish AMP, tilapia hepcidin (TH)2-3, was reported to modulate lipopolysaccharide (LPS)-induced cytokines and inhibit TNF-a through cyclooxygenase-2 and phosphodiesterase 4D in RAW264.7 cells. Those results suggest that AMPs' actions against bacterial infection need to be identified, and functional studies of immune-related gene modifications should be assessed using in vivo systems [16]. Although epinecidin-1 showed bactericidal features in most in vitro reports, screening the transcriptional profile of specific gene expressions by a microarray approach in aquatic animal systems was not done.…”

Section: Introductionmentioning

confidence: 97%

Insights into the antibacterial and immunomodulatory functions of the antimicrobial peptide, epinecidin-1, against Vibrio vulnificus infection in zebrafish

Pan

Hui

et al. 2011

Fish & Shellfish Immunology

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“…These peptides act in broad range, starting from physical barrier to cell mediated immunity (Nicolas et al 2003). During recent years, research on fish AMPs has increased (Cole et al 1997;Ravichandran et al 2010;Wimley and Hristova 2011) and among them, hepcidins are considered as multifunctional peptides discovered in few fish species (Hirono et al 2005;Kim et al 2008;Martin-Antonio et al 2009;Rajanbabu et al 2010). It is a well-known liver-synthesized AMP and principal iron-regulatory molecule that regarded as the mediator of inflammation and anaemia.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of Antimicrobial Peptide, Hepcidin in Medium Carp, Puntius sarana

Das

Mohapatra

Sahoo

2014

Int J Pept Res Ther

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Antimicrobial peptides are considered as effector immune molecules and during last decades, many workers have demonstrated the significant role and mechanism of hepcidin (hamp), an antimicrobial peptide in regulating the absorption, transport, storage and mobilization of iron. In the present work, hepcidin cDNA was cloned, sequenced and characterized in an important medium carp species, Puntius sarana, which is enlisted as a vulnerable species. The tissue specificity of hepcidin was checked in major organs of this species, and expression profile of hepcidin transcript was analyzed during ontogeny and infection against a common bacterial pathogen, Aeromonas hydrophila. Further, the antimicrobial activity of hepcidin was investigated against various Gram positive and negative bacterial strains, using the synthetic mature peptide of P. sarana. The obtained hamp amplicon produced a predicted ORF of 279 bases having 92 amino acids and the nucleotide sequence showed 99 % identity with zebrafish hepcidin. During the ontogeny, the hamp gene appeared at 6 h postfertilization and its expression remained constant towards 21 days post-fertilization and was intensely observed in liver as compared to other organs. After artificial exposure to A. hydrophila, up-regulation of the gene was marked at 3 and 6 h post-challenge, however, the same was down regulated after 24 h onwards. The synthetic hepcidin peptide exhibited broad spectrum antibacterial activity against important fish pathogens at a concentration of 17.30 lM. This preliminary study revealed that hepcidin might be playing an important role in innate defence of this fish species.

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Tilapia Hepcidin 2-3 Peptide Modulates Lipopolysaccharide-induced Cytokines and Inhibits Tumor Necrosis Factor-α through Cyclooxygenase-2 and Phosphodiesterase 4D

Cited by 42 publications

References 45 publications

Dietary administration of the probiotic SpPdp11: Effects on the intestinal microbiota and immune-related gene expression of farmed Solea senegalensis treated with oxytetracycline

Dietary administration of the probiotic SpPdp11: Effects on the intestinal microbiota and immune-related gene expression of farmed Solea senegalensis treated with oxytetracycline

Insights into the antibacterial and immunomodulatory functions of the antimicrobial peptide, epinecidin-1, against Vibrio vulnificus infection in zebrafish

Cloning and Characterization of Antimicrobial Peptide, Hepcidin in Medium Carp, Puntius sarana

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