Tightly associated cardiolipin in the bovine heart mitochondrial ATP synthase as analyzed by 31P nuclear magnetic resonance spectroscopy.

Eble, K S; Coleman, William B.; Hantgan, Roy R.; Cunningham, Carol C.

doi:10.1016/s0021-9258(17)45391-9

Cited by 213 publications

(39 citation statements)

References 30 publications

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“…Most notably, several confirmed and predicted subunits of the inner mitochondrial membrane F o F 1 -ATPase complex were severely downregulated. CL is tightly associated with the F o F 1 -ATP synthase, 58,59 and crystal structures have revealed CL binding sites in the rotor-stator interface, the dimer interface, and in a peripheral F o cavity. 59 Molecular modeling suggests that CL participates in proton translocation through the membrane domain.…”

Section: Discussionmentioning

confidence: 99%

Depletion of cardiolipin induces major changes in energy metabolism in Trypanosoma brucei bloodstream forms

et al. 2020

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The mitochondrial inner membrane glycerophospholipid cardiolipin (CL) associates with mitochondrial proteins to regulate their activities and facilitate protein complex and supercomplex formation. Loss of CL leads to destabilized respiratory complexes and mitochondrial dysfunction. The role of CL in an organism lacking a conventional electron transport chain (ETC) has not been elucidated. Trypanosoma brucei bloodstream forms use an unconventional ETC composed of glycerol-3-phosphate dehydrogenase and alternative oxidase (AOX), while the mitochondrial membrane potential (ΔΨm) is generated by the hydrolytic action of the F o F 1 -ATP synthase (aka F o F 1 -ATPase). We now report that the inducible depletion of cardiolipin synthase (TbCls) is essential for survival of T brucei bloodstream forms. Loss of CL caused a rapid drop in ATP levels and a decline in the ΔΨm. Unbiased proteomic analyses revealed a reduction in the levels of many mitochondrial proteins, most notably of F o F 1 -ATPase subunits and AOX, resulting in a strong decline of glycerol-3-phosphate-stimulated oxygen consumption.The changes in cellular respiration preceded the observed decrease in F o F 1 -ATPase stability, suggesting that the AOX-mediated ETC is the first pathway responding to the decline in CL. Select proteins and pathways involved in glucose and amino acid metabolism were upregulated to counteract the CL depletion-induced drop in cellular ATP.

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Section: Discussionmentioning

confidence: 99%

Depletion of cardiolipin induces major changes in energy metabolism in Trypanosoma brucei bloodstream forms

et al. 2020

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“…More recently, mitochondrial F 0 F 1 -ATP synthase has been proposed as a molecular component of the mPTP (44,67). CL molecules have been shown to be associated with ATP synthase and also to be required for its functioning (36). In fact, CL interacts specifically with the rotor of this multisubunit protein complex, either facilitating its rotation or participating directly in the rotation supported by the transmembrane proton motive force (35).…”

Section: And Mptp In Myocardial I/r Injurymentioning

confidence: 99%

Mitochondrial bioenergetics and cardiolipin alterations in myocardial ischemia-reperfusion injury: implications for pharmacological cardioprotection

Paradies

Ruggiero

et al. 2018

American Journal of Physiology-Heart and Circulatory Physiology

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Mitochondrial dysfunction plays a central role in myocardial ischemia/reperfusion (I/R) injury. Increased reactive oxygen species production, impaired electron transport chain activity, aberrant mitochondrial dynamics, calcium overload and opening of the mitochondrial permeability transition pore have been proposed as major contributory factors to mitochondrial dysfunction during myocardial I/R injury. Cardiolipin (CL), a mitochondria-specific phospholipid, plays a pivotal role in multiple mitochondrial bioenergetic processes, including respiration and energy conversion, in mitochondrial morphology and dynamics, as well as, in several steps of the apoptotic process. Changes in CL levels, species composition and degree of oxidation may have deleterious consequences for mitochondrial function with important implications in a variety of pathophysiological conditions, including myocardial I/R injury. In this review, we focus on the role played by cardiolipin alterations in mitochondrial dysfunction in myocardial I/R injury. Pharmacological strategies to prevent myocardial injury during I/R targeting mitochondrial cardiolipin are also examined.

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“…The nature of the lipid present at the dimer interface can thus be tentatively attributed to a CL molecule. This CL molecule would thus play a role in the dimerization of the NarGHI heterotrimer in the same way as in complexes III, IV and V (Eble et al, 1990;Lange et al, 2001;Palsdottir et al, 2003;Qin et al, 2006;Shinzawa-Itoh et al, 2007) or the ADP/ATP carrier (Nury et al, 2005). For instance, the dimeric state of the bovine complex IV is strongly stabilized by CL making extensive contacts with each monomer (Shinzawa-Itoh et al, 2007).…”

Section: Lipidsmentioning

confidence: 97%

“…At first, this peculiar lipid is found in the mitochondrial inner membrane, the chloroplast, the hydrogenosomes or the cytoplasmic membrane of prokaryotes (Depalo et al, 2004;de Andrade Rosa et al, 2006;Ventrella et al, 2007;Corcelli, 2009;. CL has also the ability to interact with a wide variety of membrane proteins in eukaryotes, most of them being related to oxidative phosphorylation or photophosphorylation, thereby modulating their catalytic activities and/or providing stability (Fry and Green, 1980;Yu and Yu, 1980;Poore and Ragan, 1982;Eble et al, 1990;Robinson et al, 1990;Schagger et al, 1990;Hayer-Hartl et al, 1992;Robinson, 1993;Beyer and Nuscher, 1996;Gomez and Robinson, 1999;Sedlak and Robinson, 1999;Depalo et al, 2004;Ventrella et al, 2007). In prokaryotes, CL has also been shown to restore the activity of a number of purified respiratory complexes such as the NADH dehydrogenase (Dancey and Shapiro, 1977;Esfahani et al, 1977;Thomson and Shapiro, 1981), the lactate dehydrogenase (Tanaka et al, 1976;Esfahani et al, 1977;Kimura and Futai, 1978), the succinate dehydrogenase (Esfahani et al, 1977;Reddy and Weber, 1986), the cytochrome bo 3 ubiquinol oxidase (Kita et al, 1984) or the nitrate reductase A (Arias-Cartin et al, 2011).…”

Section: Lipidsmentioning

confidence: 99%

Supramolecular Organization in Prokaryotic Respiratory Systems

Magalon

Arias-Cartin

Walburger

2012

Advances in Microbial Physiology

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Prokaryotes are characterized by an extreme flexibility of their respiratory systems allowing them to cope with various extreme environments. To date, supramolecular organization of respiratory systems appears as a conserved evolutionary feature as supercomplexes have been isolated in bacteria, archaea, and eukaryotes. Most of the yet identified supercomplexes in prokaryotes are involved in aerobic respiration and share similarities with those reported in mitochondria. Supercomplexes likely reflect a snapshot of the cellular respiration in a given cell population. While the exact nature of the determinants for supramolecular organization in prokaryotes is not understood, lipids, proteins, and subcellular localization can be seen as key players. Owing to the well-reported supramolecular organization of the mitochondrial respiratory chain in eukaryotes, several hypotheses have been formulated to explain the consequences of such arrangement and can be tested in the context of prokaryotes. Considering the inherent metabolic flexibility of a number of prokaryotes, cellular distribution and composition of the supramolecular assemblies should be studied in regards to environmental signals. This would pave the way to new concepts in cellular respiration.

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Tightly associated cardiolipin in the bovine heart mitochondrial ATP synthase as analyzed by 31P nuclear magnetic resonance spectroscopy.

Cited by 213 publications

References 30 publications

Depletion of cardiolipin induces major changes in energy metabolism in Trypanosoma brucei bloodstream forms

Depletion of cardiolipin induces major changes in energy metabolism in Trypanosoma brucei bloodstream forms

Mitochondrial bioenergetics and cardiolipin alterations in myocardial ischemia-reperfusion injury: implications for pharmacological cardioprotection

Supramolecular Organization in Prokaryotic Respiratory Systems

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