Tight Physical Linkage of the Nematode Resistance GeneGpa2and the Virus Resistance GeneRxon a Single Segment Introgressed from the Wild SpeciesSolanum tuberosumsubsp.andigenaCPC 1673 into Cultivated Potato

Voort, Jeroen Rouppe van der; Kanyuka, K.; Vossen, Edwin van der; Bendahmane, A.; Klein-Lankhorst, R. M.; Stiekema, Willem J.; Baulcombe, David C.; Bakker, J.C.

doi:10.1094/mpmi.1999.12.3.197

Cited by 81 publications

(51 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A HindIII BAC-library of the resistant parent (SH83-92-488) was available (Rouppe van der Voort et al 1999). This $11 genome equivalent BAC library comprises almost 98,000 BAC clones with an average insert size of 100 kb.…”

Section: Bac-library and Plasmid Isolationmentioning

confidence: 99%

Exploitation of a marker dense linkage map of potato for positional cloning of a wart disease resistance gene

Brugmans¹,

Hutten²,

Rookmaker³

et al. 2005

Theor Appl Genet

View full text Add to dashboard Cite

A marker-saturated linkage map of potato was used to genetically map a locus involved in the resistance against wart disease Synchytrium endobioticum race 1. The locus mapped on the long arm of chromosome 4 and is named Sen1-4 in contrast to a Sen1 locus on chromosome 11. The AFLP markers from the Sen1-4 interval enabled the isolation of BAC clones from an 11 genome equivalent BAC library. This was achieved via fingerprinting of BAC pools with the AFLP primer pairs that resemble the genetic marker loci. With non-selective AFLP primers, fingerprints of individual BAC clones were generated to analyse the overlap between BAC clones using FPC. This resulted in a complete contig and a minimal tiling path of 14 BAC clones enclosing the Sen1-4 locus. The BAC contig has a genetic length of $6 cM and a physical length of $1 Mb. Our results demonstrate that map-based cloning of Sen1-4 can be pursued on the basis of a strategy of marker saturation alone. Genetic resolution achieved by screening large numbers of offspring for recombination events may not be required. Together with the construction of the BAC contig, a physical map with the position of the markers is accomplished in one step. This provides proof of concept for the utility of the marker saturation that is offered by the ultra dense AFLP map of potato for gene cloning.

show abstract

Section: Bac-library and Plasmid Isolationmentioning

confidence: 99%

Exploitation of a marker dense linkage map of potato for positional cloning of a wart disease resistance gene

Brugmans¹,

Hutten²,

Rookmaker³

et al. 2005

Theor Appl Genet

View full text Add to dashboard Cite

show abstract

“…The tetraploid resistant female parent 707TG11-1 was derived from a quadruple hybrid ABPT clone (Hermsen and Ramanna 1973). The diploid susceptible male parent RH89-039-16, producing unreduced 2n pollen, is one of the advanced clones widely used for mapping research at the Laboratory of Plant Breeding and the Laboratory of Nematology, Wageningen University (Rouppe van der Voort et al 1997aVoort et al , b, 1998Voort et al , 1999Huang et al 2004;van Os et al personal communication).…”

Section: Plant Materialsmentioning

confidence: 99%

High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

et al. 2005

Self Cite

View full text Add to dashboard Cite

Introduction of more durable resistance against Phytophthora infestans causing late blight into the cultivated potato is of importance for sustainable agriculture. We identified a new monogenically inherited resistance locus that is localized on chromosome 4. The resistance is derived from an ABPT clone, which is originally a complex quadruple hybrid in which Solanum acaule, S. bulbocastanum, S. phureja and S. tuberosum were involved. Resistance data of the original resistant accessions of the wild species and analysis of mobility of AFLP markers linked to the resistance locus suggest that the resistance locus is originating from S. bulbocastanum. A population of 1383 genotypes was screened with two AFLP markers flanking the Rpi-abpt locus and 98 recombinants were identified. An accurate high-resolution map was constructed and the Rpi-abpt locus was localized in a 0.5 cM interval. One AFLP marker was found to cosegregate with the Rpi-abpt locus. Its DNA sequence was highly similar with sequences found on a tomato BAC containing several resistance gene analogues on chromosome 4 and its translated protein sequence appeared to be homologous to several disease resistance related proteins. The results indicated that the Rpi-abpt gene is a member of an R gene cluster.

show abstract

“…Gpa2, which confers resistance against the potato cyst nematode Globodera pillida, was found to be tightly linked in the potato genome to Rx, which is responsible for resistance against potato virus X (PVX; van der Voort et al, 1999). Their cloning and molecular characterization indicate that they are highly similar at the amino acid level and thus form a single R gene family (Bendahmane et al, 1999; J. Bakker, personal communication).HRT appears to have been formed in part by an unequal crossing over between progenitor genes related to RPP8 Inoculated leaves were stained with trypan blue to show the relative size of necrotic tissue at 3 DPI. RNA isolated from uninoculated (U) leaves at 6 DPI was analyzed for PR-1 induction, systemic spread of TCV, and HRT expression.…”

mentioning

confidence: 99%

“…This HR Ϫ resistance is reminiscent of the extreme resistance exhibited by potatoes carrying Rx after infection with PVX. In Rx-containing potato plants, the rapid arrest of PVX replication in the initially infected cells appears to prevent development of an HR (Kohm et al, 1993;Bendahmane et al, 1999).Analysis of HRT mRNA levels in the transformed lines revealed that the extent of HRT expression directly correlated with the extent of TCV resistance and was inversely proportional to lesion size. Plants of the HR Ϫ but extreme resistant lines expressed three-to fivefold more HRT than did Di-17 plants ( Figure 5), whereas lines exhibiting a micro-HR and partial resistance had intermediate HRT expression.…”

mentioning

confidence: 99%

Members of the Arabidopsis HRT/RPP8 Family of Resistance Genes Confer Resistance to Both Viral and Oomycete Pathogens

et al. 2000

View full text Add to dashboard Cite

Turnip crinkle virus (TCV) inoculation onto TCV-resistant Arabidopsis leads to a hypersensitive response (HR) controlled by the dominant gene HRT . HRT is a member of the class of resistance ( R ) genes that contain a leucine zipper, a nucleotide binding site, and leucine-rich repeats. The chromosomal position of HRT and its homology to resistance gene RPP8 and two RPP8 homologs indicate that unequal crossing over and gene conversion may have contributed to HRT evolution. RPP8 confers resistance to an oomycete pathogen, Peronospora parasitica . Despite very strong similarities within the HRT/RPP8 family, HRT and RPP8 are specific for the respective pathogens they detect. Hence, the HRT/ RPP8 family provides molecular evidence that sequence changes between closely related members of multigene families can generate novel specificities for radically different pathogens. Transgenic plants expressing HRT developed an HR but generally remained susceptible to TCV because of a second gene, RRT , that regulates resistance to TCV. However, several transgenic plants that overexpressed HRT produced micro-HRs or no HR when inoculated with TCV and were resistant to infection. Expression of the TCV coat protein gene in seedlings containing HRT resulted in massive necrosis and death, indicating that the avirulence factor detected by the HRT -encoded protein is the TCV coat protein. INTRODUCTIONTo survive, plants must defend themselves against numerous pathogens. Some defenses are constitutive, such as various preformed antimicrobial compounds (Osbourn, 1996), whereas others are activated by recognition of pathogens (Hammond-Kosack and Jones, 1996;Yang et al., 1997). The key players in this recognition process include the product of a dominant or semidominant resistance ( R ) gene present in the plant and the corresponding dominant avirulence (Avr) factor encoded by or derived from the pathogen. Recognition of the Avr factor by the host plant initiates one or more signal transduction pathways that activate various plant defenses and thus compromise the ability of the pathogen to colonize the plant. In this gene-for-gene interaction, the prevailing thought is that the R protein either acts directly as the receptor of the Avr factor (Ellingboe, 1980; Bent, 1996;Yang et al., 1997) or recognizes the Avr factor indirectly through a coreceptor (Dixon et al., 1998). To date, direct interaction between an R protein and an Avr factor has been demonstrated only for the tomato Pto and the Pseudomonas syringae AvrPto proteins (Scofield et al., 1996;Tang et al., 1996) and between the rice Pi-ta and the Magnaporthe grisea AvrPita proteins (Jia et al., 1999).An array of R genes that provide protection against viruses, bacteria, fungi, and oomycetes has been cloned from both monocots and dicots during the past 6 years (Staskawicz et al., 1995; Bent, 1996; Baker et al., 1997; DeWit, 1997). Many contain a nucleotide binding site (NBS). Often located closer to the N terminus of the R protein is either a leucine zipper or a TIR domain, which is sim...

show abstract

Tight Physical Linkage of the Nematode Resistance GeneGpa2and the Virus Resistance GeneRxon a Single Segment Introgressed from the Wild SpeciesSolanum tuberosumsubsp.andigenaCPC 1673 into Cultivated Potato

Cited by 81 publications

References 60 publications

Exploitation of a marker dense linkage map of potato for positional cloning of a wart disease resistance gene

Exploitation of a marker dense linkage map of potato for positional cloning of a wart disease resistance gene

High-resolution Mapping and Analysis of the Resistance Locus Rpi-abpt Against Phytophthora infestans in Potato

Members of the Arabidopsis HRT/RPP8 Family of Resistance Genes Confer Resistance to Both Viral and Oomycete Pathogens

Contact Info

Product

Resources

About