Tick-borne infectious diseases of dogs

Shaw, Susan E.; Day, Michael; Birtles, Richard J.; Breitschwerdt, Edward B.

doi:10.1016/s1471-4922(00)01856-0

Cited by 254 publications

(223 citation statements)

References 39 publications

Supporting

Mentioning

210

Contrasting

Unclassified

Order By: Relevance

“…On the basis of present PCR results, it could be mentioned that highest number (45.94 %) of positive cases having mixed infection of B. canis and B. gibsoni. This is important for the purpose of obtaining maximum response to therapy in clinical cases as observed by Shaw et al (2001) and Walter et al (2002) who reported drug regimen against the two species were different and treatment against one species might be unsuccessful due to usual presence of mixed infection. Molecular analysis in the present study provided powerful information over blood smears examination, which did not provide definite diagnostic information on B. canis compared to that of B. gibsoni.…”

Section: Resultsmentioning

confidence: 99%

Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction

Laha

Bhattacharjee²,

Sarmah³

et al. 2013

J Parasit Dis

View full text Add to dashboard Cite

The objective of the study was to detect Babesia infections in pet dogs of a north-eastern state of India. The diagnostic efficacy of Babesia infection by polymerase chain reaction (PCR) technique has been compared with microscopy examination. For this, a total of 111 blood samples of pet dogs presented at clinical complex of the College of Veterinary Science, Guwahati, Assam with clinical signs suspected for Babesia infection subjected to the study. A total of 44 (39.63 %) dogs were diagnosed as positive for Babesia infections after microscopic examination. Among these, Babesia canis infection was diagnosed in 5 dogs (4.50 %) and B. gibsoni infection in 39 (35.13 %) dogs microscopically in Giemsa stained blood smears. Molecular diagnosis using PCR detected 63 (56.75 %) dogs positive for Babesia infection. Single infection with B. canis was found in 9 (8.10 %) dogs while B. gibsoni alone was detected in 3 (2.70 %) dogs. Mixed infections by both these species were detected in 51 (45.94 %) dogs. Overall, PCR detected 54 (48.64 %) dogs as B. gibsoni and 60 (54.05 %) dogs as B. canis positive.

show abstract

Section: Resultsmentioning

confidence: 99%

Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction

Laha

Bhattacharjee²,

Sarmah³

et al. 2013

J Parasit Dis

View full text Add to dashboard Cite

show abstract

“…However, the rate of 8.6% of PCR-positive cats in two target genes highlights the potential infection by E. canis in the feline population of Cuiabá, which is a known E. canis epidemic region in Brazil (SILVA et al, 2010;ALMEIDA et al, 2012;MELO et al, 2011). Clinical and laboratory manifestations suggestive of ehrlichiosis have been reported in cats (BREITSCHWERDT et al, 2002) and, unlike CME, little is known about the pathogenesis or immunopathological implications resulting from chronic infection in cats (SHAW et al, 2001). The PCR results revealed that positive cats have a tendency for lymphopenia (p= 0.07), a finding that is commonly reported in animals in the acute phase of infection (HARRUS et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Symptoms suggestive of ehrlichiosis infection have also been reported in domestic cats in several countries. The Ehrlichia species that naturally infect cats have not yet been fully determined, although monocyte and lymphocyte inclusions and, more consistently, E. canis DNA, have been detected in cats (BREITSCHWERDT et al, 2002;OLIVEIRA et al, 2009;BRAGA et al, 2012), as well as some granulocytic inclusions related to feline granulocytic ehrlichiosis caused by Anaplasma phagocytophilum (BUORO et al, 1989;BOULOY et al, 1994;BEAUFILS et al, 1995;BJOERSDORFF et al, 1999;SHAW et al, 2001;AGUIRRE et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Hematological values associated to the serological and molecular diagnostic in cats suspected of Ehrlichia canisinfection

Braga

Santos

Melo

et al. 2013

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

The literature contains several studies on feline ehrlichiosis. However, information about the characteristics of Ehrlichia infection in cats is still scanty. This study evaluated the association between Ehrlichia spp. infection and the hematologic data of 93 cats treated at the Federal University of Mato Grosso Veterinary Hospital in Cuiabá, state of Mato Grosso, Brazil. The presence of or exposure to Ehrlichia spp. infection was evaluated by Polymerase Chain Reaction (PCR) targeting the dsb and 16S rRNA gene of Ehrlichia, and by detection of anti-Ehrlichia canis IgG antibodies in Indirect Fluorescence Assay (IFA), respectively. Eight (8.6%) cats tested positive by PCR and the partial DNA sequence obtained from PCR products was a 100% match to E. canis. Forty-two (45.1%) cats showed antibody reactivity against Ehrlichia spp. Hematological alterations such as low erythrocyte count, thrombocytopenia, lymphopenia and monocytosis were observed in PCR positive cats. Among them, low erythrocyte counts were associated with IgG antibody titers of 40 to 640 and five cats also tested positive by PCR. Furthermore, PCR-positive cats showed a tendency to be lymphopenic. No correlation was found between age and sex, and no ticks were observed in any of the examined cats.Keywords: Ehrlichiosis, feline, hematology, polymerase chain reaction, indirect fluorescence assay, diagnosis. ResumoDiversos estudos sobre erliquiose felina vêm sendo relatados na literatura. No entanto, a caracterização da infecção por Ehrlichia em gatos ainda é escassa. O presente estudo objetivou avaliar a associação entre infecção por Ehrlichia e dados hematológicos em 93 gatos atendidos no Hospital Veterinário da Universidade Federal de Mato Grosso, em Cuiabá, Brasil. A presença de infecção por Ehrlichia spp. foi avaliada pela Reação em Cadeia pela Polimerase (PCR) visando à amplificação dos genes dsb e 16S rRNA de Ehrlichia e por Reação de Imunofluorescência Indireta (RIFI). Oito (8,6%) gatos demonstraram ser positivos pela PCR, sendo suas sequências parciais de DNA 100% idênticas à E. canis. Quarenta e dois gatos (45,1%) apresentaram anticorpos reativos contra Ehrlichia spp. Alterações hematológicas como baixas contagens de eritrócitos, trombocitopenia, linfopenia e monocitose foram observadas em gatos positivos pela PCR. Dentre essas, eritropenia foi associada em gatos com títulos de anticorpos IgG entre 40 e 640, sendo cinco destes positivos pela PCR. Adicionalmente, gatos positivos na PCR apresentaram uma tendência a serem linfopênicos. Não foram observadas associações entre a presença de infecção nos gatos e suas respectivas idades e sexo. Nenhum carrapato foi observado nos gatos examinados.Palavras-chave: Erliquiose, felinos, hematologia, reação em cadeia pela Polimerase, reação de imunofluorescência indireta, diagnóstico.

show abstract

“…Many studies have reported on the seasonality of the onset of IMHA (Jackson and Kruth 1985;Klag et al 1993;Duval and Giger 1996;Burgess et al 2000;Weinkle et al 2005;McAlees 2010) with increased exposure to infectious agents in certain seasons being suggested to trigger the disease. The occurrence of IMHA secondary to vector-borne diseases affects dogs that live or have visited the geographic area where the vector is endemic (Morita et al 1995;Frank and Breitschwerdt 1999;Shaw et al 2001). Inconsistencies in disease incidence between countries might be explained by differences in exposure risk in different geographic locations.…”

Section: Diagnostic Testingmentioning

confidence: 99%

Canine idiopathic immune-mediated haemolytic anaemia: a review with recommendations for future research

Piek

2011

Veterinary Quarterly

View full text Add to dashboard Cite

Tick-borne infectious diseases of dogs

Cited by 254 publications

References 39 publications

Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction

Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction

Hematological values associated to the serological and molecular diagnostic in cats suspected of Ehrlichia canisinfection

Canine idiopathic immune-mediated haemolytic anaemia: a review with recommendations for future research

Contact Info

Product

Resources

About