Tiber Aufbau und Abbau des Glykogens durch Leukocyten. (Zehnte Abhandlung über Enzyme der Leukocyten.)

Willstätter, Richard; Rohdewald, Margarete

doi:10.1515/bchm2.1937.247.3.115

Cited by 16 publications

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“…This finding corresponds to the original observation of E h r l ic h and L azarus (2) that neutrophils in the peripheral blood from normal subjects are iodine-negative, in spite of the fact that the presence of glycogen in these cells was later demonstrated biochemically and histochcmically. The absence of iodophilia has been ascribed to a binding between glycogen and cell protein (12,13). The neutro phils in purulent exudates are, however, found to be iodophilic (1,3), but in the "skin window" only the initial metabolic changes can be observed, because of the repeated removal of cells from the lesion.…”

Section: Commentmentioning

confidence: 99%

Histochemical Studies of Leukocytes from an Inflammatory Exudate. Glycogen and Phosphorylase

Wulff¹

1962

Acta Haematol

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Section: Commentmentioning

confidence: 99%

Histochemical Studies of Leukocytes from an Inflammatory Exudate. Glycogen and Phosphorylase

Wulff¹

1962

Acta Haematol

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“…The method, frequently applied (18,19,20,27), of determining aerobic lactic acid formation by measuring the sugar consumption is inadequate, since the measured decrease of the sugar concentration does not indicate how much of the sugar has been used in respiration or in the formation of glycogen (30), and how much has been actually split into lactic acid.…”

mentioning

confidence: 99%

The Nature of Leukemic Blood Cells as Determined by Their Metabolism

Kempner

1939

J. Clin. Invest.

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" The nature and etiology of leukemia are unknown " (1). Some authors (2,3,4,5,6,7,8) are in favor of classifying the leukemias as malignant neoplasms of the blood-forming organs, because of the formation of leukocytes in unaccustomed organs, the new growth of leukemic tissue, and the flooding of the blood stream with abnormal primitive cells. Others (9) prefer to consider leukemia as a type of benign tumor formation, or tissue metaplasia, or simply as an excessive, unregulated production and outpouring of essentially normal young tissue cells. To solve the problem by means of morphological histology has proved impossible (10).A new approach to this question was opened, however, by the methods of cellular physiology, and this paper presents experimental results obtained by these methods, which, it seems to me, give an unambiguous and definite answer to the question of the nature of leukemic cells.As Warburg (11) has found, cells of benign, and to an even greater degree of malignant tumors, differ fundamentally from normal cells in their energy supplying metabolic reactions. In all normal undamaged animal cells the magnitude of the oxidative metabolism is considerably greater than the magnitude of the glycolytic metabolism and, under aerobic conditions, no splitting of sugar into lactic acid (glycolysis) occurs. In the metabolism of benign tumor cells and even more of cancer cells, the respiration-glycolysis ratio is disturbed; the rate of glycolysis is high as compared to the rate of respiration and a great amount of lactic acid is formed under aerobic conditions. This aerobic glycolysis, however, may have a different origin; it can be produced, for example, by poisoning the cells with cyanide, carbon monoxide, or arsenious acid, by various injuries leading to the gradual death of the cell, or, in the more sensitive organs, simply by keeping the tissue in Ringer's solution instead of its own plasma. Aerobic glycolysis as such, then, is not specific for malignancy, but rather the fact that the cancer Common to all these experiments with mature animal leukocytes of various types, was the ambiguous result that the fermentative metabolism was high in comparison to the oxidative metabolism and that splitting of sugar into lactic acid took place under aerobic conditions. The original question remained unsolved, whether this me-291

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