Der Zusammenhang von Kalium‐ und Kohlehydratstoffwechsel bei der Hefe

Pulver, R; Verzár, F.

doi:10.1002/hlca.194002301132

Cited by 42 publications

(2 citation statements)

References 6 publications

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“…The further addition of glucose and PO, causes a K influx leading to final K levels of more than 150 per cent of normal. A relationship between K and carbohydrate metabolism in yeast has been frequently observed in the past (15,16).…”

Section: Discussionmentioning

confidence: 97%

Cation Transport in Yeast

Foulkes

1956

The Journal of General Physiology

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1. The distribution of azide added to suspensions of bakers' yeast was studied under various conditions. The recovery of azide was estimated in the volume of water into which low concentrations of electrolytes can readily diffuse (anion space). Considerable azide disappeared from this anion space. 2. The incomplete recovery of azide in the anion space is due to its uptake by the cells. This uptake occurs against a concentration gradient at 0°C., and is attributed to binding of azide by cell constituents. 3. Confirmatory evidence is presented that one such constituent is the K carrier in the cell membrane. The azide inhibition of K transport is not mediated by inhibition of cytochrome oxidase in the mitochondria. 4. From the amount of combined azide and the experimentally determined dissociation constant of the K carrier-inhibitor complex, the maximum value for the concentration of this carrier is calculated as 0.1 µM/gm. yeast. 5. The addition of glucose and PO4 causes a secondary K uptake which is not azide-sensitive and is clearly distinct from the primary, azide-sensitive mechanism. 6. The existence of a separate carrier responsible for Na extrusion is reconsidered. It is concluded that present evidence does not necessitate the assumption that such a carrier is active in yeast.

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Section: Discussionmentioning

confidence: 97%

Cation Transport in Yeast

Foulkes

1956

The Journal of General Physiology

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“…The electrical compensation for H+ extruded under these conditions is not clear. In S. cerevisiae an efflux of organic anions occurs (Pulver & Verzar, 1940;Rothstein & Bruce, 1958;Sigler et al, 198 1) which could compensate for the charge translocation by the ATPase.…”

Section: Comparison Of the Eflect Of Glucose And Of Ethanol On Cell Mmentioning

confidence: 99%

Effect of ethanol on cell growth, metabolism and cation fluxes in the yeast Metschnikowia reukaufii

Gläser

Höfer

1990

Journal of General Microbiology

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The yeast Metschnikowia reukaufii could not utilize ethanol as a growth substrate, although ethanol stimulated cellular O2 consumption and the reduction of intracellular nicotinamide adenine nucleotides. Also, ethanol inhibited cell growth in glucose-containing medium. The effect was stronger when the K+ concentration in the growth medium was lowered from 5.8 to 0.6 mM. Addition of glucose to an aerated cell suspension caused an initial H+ efflux and K+ influx in a ratio of approximately 1 : 1. This was followed by a phase of continuing extracellular acidification without any measurable uptake of K+. In contrast, in cells energized by glucose, ethanol stimulated K+ efflux; concomitantly, H+ extrusion was markedly lowered by ethanol. The rates of H+ extrusion correlated with the intracellular level of glucose 6-phosphate and not of ATP. It is concluded that there is a regulatory interaction, though not by a direct effect, between glucose 6-phosphate and the plasma-membrane ATPase. Ethanol appears to activate electron transfer from cytosolic NADH to O2 by a pathway independent of the mitochondria1 respiratory chain.

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Permeability and Enzyme Reactions

Brooks

1947

Advances in Enzymology - And Related Areas of Molecular Biology

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Der Zusammenhang von Kalium‐ und Kohlehydratstoffwechsel bei der Hefe

Cited by 42 publications

References 6 publications

Cation Transport in Yeast

Cation Transport in Yeast

Effect of ethanol on cell growth, metabolism and cation fluxes in the yeast Metschnikowia reukaufii

Permeability and Enzyme Reactions

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