Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity.

Guazzi, Stefania; Price, Melanie; Felice, Mario De; Damante, Giuseppe; Mattei, M G; Lauro, Roberto Di

doi:10.1002/j.1460-2075.1990.tb07574.x

Cited by 522 publications

(380 citation statements)

References 41 publications

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“…23 The importance of these three transcription factors is evidenced by congenital thyroid agenesis, which results from mutations in any of the transcription factors. [26][27][28][29][30][31][32] We found consistent nuclear expressions of Pax8, TTF-1, and TTF-2 in well-differentiated neoplasms of follicular cell origin, that is, papillary carcinomas, follicular adenomas, follicular carcinomas, and poorly differentiated carcinomas. Seventy-nine percent of anaplastic carcinomas variably showed Pax8 reaction, whereas TTF-1 and TTF2 were expressed in only 18 and 7% of anaplastic carcinomas, respectively.…”

Section: Discussionmentioning

confidence: 81%

Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms

et al. 2008

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Thyroid-specific transcription factors, Pax8, TTF-1, and TTF-2, are crucial for thyroid organogenesis and differentiation. Compared with TTF-1, the other two markers have scarcely been investigated in surgical pathology. The goal of this study is to evaluate the expressions of these markers in thyroid tumors of the full spectrum of differentiation, with special emphasis on anaplastic carcinomas. A total of 94 cases of thyroid neoplasms were studied: 17 papillary carcinomas, 18 follicular adenomas, 16 follicular carcinomas, 7 poorly differentiated carcinomas, 28 anaplastic carcinomas, and 8 medullary carcinomas. Immunostains for these three markers were performed. The antibodies to Pax8 and TTF-2 were also applied on 147 lung carcinomas as well as a variety of normal tissues and malignant tumors. All three markers were seen in papillary carcinomas, follicular adenomas and carcinomas, and poorly differentiated carcinomas in a diffuse manner, whereas their expressions in medullary carcinomas were variable. Pax8 was expressed in 79% of anaplastic carcinomas to a variable extent, whereas TTF-1 and TTF-2 were seen only in 18 and 7% of anaplastic carcinomas, respectively. TTF-2 was negative in all other neoplastic and non-neoplastic tissues including those of the lung. Pax8 was expressed in renal tubules, fallopian tubes, ovarian inclusion cysts, and lymphoid follicles as well as renal carcinoma, nephroblastoma, seminoma, and ovarian carcinoma, but not in normal tissue and carcinomas of the lung. Pax8 is a useful marker for the diagnosis of anaplastic carcinomas, particularly when the differential diagnosis includes pulmonary carcinoma. In differentiated thyroid neoplasms, no significant difference in expression was seen in all the three transcription factors.

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Section: Discussionmentioning

confidence: 81%

Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms

et al. 2008

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“…Rabbit polyclonal antibody generated against rat HNF-3␤ and used at a dilution of 1:8000 (Jacob et al, 1994). Rabbit antiserum against rat TTF-1 was kindly provided by Dr. Roberto DiLauro (Guazzi et al, 1990) and used at dilution 1:8000. Rabbit antiserum against rat CCSP was the kind gift of Dr. Gurmukh Singh (Department of Veterans Affairs Medical Center, Pittsburgh, PA) and used at a dilution of 1:10,000.…”

Section: Antibodies and Immunohistochemistrymentioning

confidence: 99%

TGF‐β1 perturbs vascular development and inhibits epithelial differentiation in fetal lung in vivo

Zeng

Gray

Stahlman

et al. 2001

Developmental Dynamics

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Members of the transforming growth factor ␤ (TGF-␤) family of polypeptides have been implicated in morphogenesis and differentiation in numerous tissues, including the lung. In order to further define effects of TGF-␤ signaling in lung morphogenesis, a constitutively active form of TGF-␤1 was expressed in respiratory epithelial cells of the fetal mouse lung in vivo. Expression of TGF-␤1 arrested lung morphogenesis in the pseudoglandular stage of development, inhibiting synthesis of differentiation-dependent proteins, SP-B, SP-C, and CCSP, and maintaining embryonic patterns of staining for thyroid transcription factor-1 (TTF-1) and hepatocyte nuclear factor-3␤ (HNF-3␤). The pulmonary mesenchyme was thickened and vascular density was increased by TGF-␤1. TGF-␤1 decreased expression of vascular endothelial growth factor-A (VEGF-A) mRNA and protein, and the abundance of Flk-1 mRNA in the lung mesenchyme. Distribution of platelet-endothelial cell adhesion molecule (PECAM)-1, a marker of pulmonary blood vessels, was altered, and ultrastructural studies demonstrated that TGF-␤1 inhibited vascular development in the fetal lung. TGF-␤1 perturbed both epithelial cell differentiation and formation of the pulmonary vasculature, supporting the concept that precise control of signaling via the TGF-␤ receptor pathway is critical for normal lung morphogenesis.

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“…A homeodomain transcription factor, T/ebp (also called Ttf1, Titf1, or Nkx2.1) (Guazzi et al, 1990;Mizuno et al, 1991), is one of the transcription factors essential for regulating the expression of genes involved in thyroid hormone synthesis as well as thyroid organogenesis (Kimura et al, 1996;De Felice et al, 1998;Mansouri et al, 1998;Damante et al, 2001;Di Lauro and De Felice, 2001;De Felice and Di Lauro, 2004). Mice carrying a null mutation for T/ebp gene die at birth due to profoundly hypoplastic lungs, a defective hypothalamus, and absence of the thyroid and pituitary glands (Kimura et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Origin of the ultimobranchial body cyst: T/ebp/Nkx2.1 expression is required for development and fusion of the ultimobranchial body to the thyroid

Kusakabe

Hoshi

Kimura

2005

Developmental Dynamics

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Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity.

Cited by 522 publications

References 41 publications

Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms

Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms

TGF‐β1 perturbs vascular development and inhibits epithelial differentiation in fetal lung in vivo

Origin of the ultimobranchial body cyst: T/ebp/Nkx2.1 expression is required for development and fusion of the ultimobranchial body to the thyroid

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