Origin of the ultimobranchial body cyst: T/ebp/Nkx2.1 expression is required for development and fusion of the ultimobranchial body to the thyroid

Kusakabe, Takashi; Hoshi, Nobuo; Kimura, Shioko

doi:10.1002/dvdy.20655

Cited by 52 publications

(72 citation statements)

References 41 publications

(73 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ttf-1 is expressed in the ultimobranchial body, and it was proposed that Ttf-1 may regulate the calcitonin gene in the maintenance of calcium homeostasis in C cells. 36,37 Pax8 expression was also demonstrated in the ultimobranchial body, but its role in C cells remains largely unclear. 23 A relation of Ttf-2 to the ultimobranchial body has not been reported to our knowledge.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms

et al. 2008

View full text Add to dashboard Cite

Thyroid-specific transcription factors, Pax8, TTF-1, and TTF-2, are crucial for thyroid organogenesis and differentiation. Compared with TTF-1, the other two markers have scarcely been investigated in surgical pathology. The goal of this study is to evaluate the expressions of these markers in thyroid tumors of the full spectrum of differentiation, with special emphasis on anaplastic carcinomas. A total of 94 cases of thyroid neoplasms were studied: 17 papillary carcinomas, 18 follicular adenomas, 16 follicular carcinomas, 7 poorly differentiated carcinomas, 28 anaplastic carcinomas, and 8 medullary carcinomas. Immunostains for these three markers were performed. The antibodies to Pax8 and TTF-2 were also applied on 147 lung carcinomas as well as a variety of normal tissues and malignant tumors. All three markers were seen in papillary carcinomas, follicular adenomas and carcinomas, and poorly differentiated carcinomas in a diffuse manner, whereas their expressions in medullary carcinomas were variable. Pax8 was expressed in 79% of anaplastic carcinomas to a variable extent, whereas TTF-1 and TTF-2 were seen only in 18 and 7% of anaplastic carcinomas, respectively. TTF-2 was negative in all other neoplastic and non-neoplastic tissues including those of the lung. Pax8 was expressed in renal tubules, fallopian tubes, ovarian inclusion cysts, and lymphoid follicles as well as renal carcinoma, nephroblastoma, seminoma, and ovarian carcinoma, but not in normal tissue and carcinomas of the lung. Pax8 is a useful marker for the diagnosis of anaplastic carcinomas, particularly when the differential diagnosis includes pulmonary carcinoma. In differentiated thyroid neoplasms, no significant difference in expression was seen in all the three transcription factors.

show abstract

Section: Discussionmentioning

confidence: 99%

Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Nevertheless, TG expressing follicles also differentiate within the C-cell clusters (Kameda and Ikeda, 1980). Murine ultimobranchial bodies contain a second poorly characterized cell type in addition to C-cells that might act as a stem cell pool capable of differentiating into both C-cells and thyroid follicle cells (Kusakabe et al, 2005). Whereas in mammals the contribution of cell lineages to thyroid cell types remains controversial, we can exclude the possibility that neural crest cells for zebrafish contribute to follicle cells at least during the early larval stages we analyzed.…”

Section: Discussionmentioning

confidence: 99%

“…The role of Nkx2.1 in the development of the diverticulum and physiological activity of thyroid follicle cells is well understood (reviewed in De Felice and Di Lauro, 2004). In the ultimobranchial bodies, Nkx2.1 is required for C-cell differentiation and for fusion with the thyroid lobes (Kusakabe et al, 2005). The zebrafish orthologue nk2.1a is expressed in the thyroid diverticulum and has a conserved function with respect to thyroid follicle development .…”

Section: Discussionmentioning

confidence: 99%

“…These bodies occur as a bilateral pair of epithelial bodies that can be found earlier associated with the most posterior pair of branchial pouches (Pearse and Carvalheira, 1967). After fusion of ultimobranchial bodies and diverticulum, the gland's cells differentiate into follicles and C-cells (Kusakabe et al, 2005;Fagman et al, 2006). It is widely believed that the ultimobranchial bodies give rise to C-cells and the diverticulum to thyroid follicle cells.…”

Section: Introductionmentioning

confidence: 99%

“…It is widely believed that the ultimobranchial bodies give rise to C-cells and the diverticulum to thyroid follicle cells. However, due to the late time point of differentiation into both cell types after fusion of the primordia and the lack of lineage analyses, it remains unclear if such a strict separation into lineages exists (Manley and Capecchi, 1998;Kusakabe et al, 2005 and references herein).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of origin and growth of the thyroid gland in zebrafish

Alt

Reibe

Feitosa

et al. 2006

Developmental Dynamics

153

141

View full text Add to dashboard Cite

The zebrafish thyroid gland shows a unique pattern of growth as a differentiated endocrine gland. Here, we analyze the onset of differentiation, the contribution of lineages, and the mode of growth of this gland. The expression of genes involved in hormone production and the establishment of epithelial polarity show that differentiation into a first thyroid follicle takes place early during embryonic development. Thyroid follicular tissue then grows along the pharyngeal midline, initially independently of thyroid stimulating hormone. Lineage analysis reveals that thyroid follicle cells are exclusively recruited from the pharyngeal endoderm. The ultimobranchial bodies that merge with the thyroid in mammals form separate glands in zebrafish as visualized by calcitonin precursor gene expression. Mosaic analysis suggests that the first thyroid follicle differentiating at 55 hours postfertilization corresponds later to the most anterior follicle and that new follicles are added caudally.

show abstract

Expression of Islet1 in thyroid development related to budding, migration, and fusion of primordia

Westerlund¹,

Andersson²,

Carlsson³

et al. 2008

Developmental Dynamics

View full text Add to dashboard Cite

The LIM homeodomain transcription factor Isl1 was investigated in mouse thyroid organogenesis. All progenitor cells of the midline thyroid diverticulum and lateral primordia (ultimobranchial bodies) expressed Isl1. This pattern persisted until the growing anlagen fused at embryonic day (E) 13.5. In Isl1 null mutants thyroid progenitors expressing Nkx2.1 and Pax8 were readily specified in the anterior endoderm but the size of the thyroid rudiment was reduced. In late development, only immature C-cells expressed Isl1. In the adult gland the number of Isl1؉ cells was small compared with cells expressing calcitonin. Analysis of microarray profiles indicated a higher level of Isl1 expression in medullary thyroid carcinomas than in tumors derived from follicular cells. Together, these findings suggest that Isl1 may be a novel regulator of thyroid development before terminal differentiation of the endocrine cell types. Isl1 is an embryonic C-cell precursor marker that may be relevant also in cancer developed from the mature C-cell. Developmental Dynamics 237:3820 -3829, 2008.

show abstract

Origin of the ultimobranchial body cyst: T/ebp/Nkx2.1 expression is required for development and fusion of the ultimobranchial body to the thyroid

Abstract: The ultimobranchial body (UBB) is an outpocketing of the fourth pharyngeal pouch that fuses with the thyroid diverticulum, giving rise to calcitonin-producing C-cells. In this study, we demonstrate that the UBB is composed of two types

Cited by 52 publications

References 41 publications

Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms

Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms

Analysis of origin and growth of the thyroid gland in zebrafish

Expression of Islet1 in thyroid development related to budding, migration, and fusion of primordia

Contact Info

Product

Resources

About