Thyroid hormone enhances stem cell maintenance and promotes lineage-specific differentiation in human embryonic stem cells

Deng, Chunhao; Zhang, Zhaoying; Xu, Faxiang; Xu, Jiaqi; Ren, Zhili; Godoy-Parejo, Carlos; Xiao, Xiangming; Liu, Weiwei; Zhou, Zhou; Chen, Guokai

doi:10.1186/s13287-022-02799-y

Cited by 8 publications

(6 citation statements)

References 55 publications

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“…Other studies prove that the influence of thyroid hormones on VEGF expression is related not so much to the function of the hormones as to the amount of endocrine-active tissue. 39,40 In our study, we observed that the expression of VEGFA and VEGFC genes in the MSC of the umbilical cord collected from hypertensive women is statistically significantly higher compared to healthy women. A team of researchers from India assessed the expression of VEGF in umbilical cord tissues depending on the severity of hypertension.…”

Section: Discussionmentioning

confidence: 54%

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VEGF Expression in Umbilical Cord MSC Depends on the Patient’s Health, the Week of Pregnancy in Which the Delivery Took Place, and the Body Weight of the Newborn – Preliminary Report

Bieńko¹,

Wójcik²,

Więckowska³

et al. 2023

SCCAA

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Introduction: Cells collected from Wharton's jelly are a rich source of mesenchymal stem cells. They can be easily obtained and grown using the adhesive method. They produce many types of proteins, including VEGF. Their role is to participate in angiogenesis, vasodilation, stimulation of cells to migrate, and chemotactic activity. The aim of this study was to evaluate expression of genes from the vascular endothelial growth factor family: VEGFA, VEGFB and VEGFC in MSC and the analysis of dependence of the expression of the studied genes on clinical factors related to the course of pregnancy and childbirth, and health of mother and child. Material and Methods: The research material was an umbilical cord obtained from 40 patients hospitalized in the Department of Obstetrics and Pathology of Pregnancy of the Independent Public Clinical Hospital No.1 in Lublin. The age of the women was 21-46, all gave birth by cesarean section. Some of the patients suffered from hypertension and hypothyroidism. Material collected from patients immediately after delivery was subjected to enzymatic digestion with type I collagenase. The isolated cells were then cultured in adherent conditions, and then gene expression was assessed using qPCR and the immunophenotype of the cells was assessed cytometrically.Results: Conducted studies have shown significant differences in expression of VEGF family genes depending on clinical condition of mother and child. Significant differences in VEGF-family gene expression level in umbilical cord MSC collected from women with hypothyroidism, hypertension, time of labor and birth weight of the baby were shown. Conclusion:Probably due to hypoxia (caused, for example, by hypothyroidism or hypertension), the MSCs found in the umbilical cord may react with an increased expression of VEGF and a compensatory increase in the amount of secreted factor, the aim of which is, i.a., vasodilation and increase of blood supply to the fetus through the umbilical vessels.

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Section: Discussionmentioning

confidence: 54%

“…Other studies prove that the influence of thyroid hormones on VEGF expression is related not so much to the function of the hormones as to the amount of endocrine-active tissue. 39 , 40 …”

Section: Discussionmentioning

confidence: 99%

VEGF Expression in Umbilical Cord MSC Depends on the Patient’s Health, the Week of Pregnancy in Which the Delivery Took Place, and the Body Weight of the Newborn – Preliminary Report

Bieńko¹,

Wójcik²,

Więckowska³

et al. 2023

SCCAA

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“…Common upregulated DEGs included TP63 and TGM6 54 , which were involved in response to environmental in ammation and ER stress. DIO2, which converts thyroxine (T4) to triiodothyronine (T3), is crucial for stem cell maintenance and TE lineage differentiation 55 . The common downregulated DEGs included DDIT4 and DUT, which are involved in key recovery mechanisms in response to cell damage.…”

Section: Differential Expression (De) Analyses Revealed Distinct Patt...mentioning

confidence: 99%

“…CellChat package (version 1.1.0) 55 was used to infer the global cell-cell communication in euploid and aneuploid cells of the 3 mosaic embryos, based on ligand-receptor interactions. We followed default parameters except for the application of projectData function, which projects gene expression data onto protein-protein interaction (PPI) networks.…”

Section: Ligand-receptor Interactome Analysismentioning

confidence: 99%

Single-cell Transcriptomics of Postimplantation Embryos: Unveiling Aneuploidy Effects and Lineage Dynamics

Kwok,

Haham,

Russell

et al. 2024

Preprint

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Transfer of aneuploid human embryos, as defined by the presence of > 80% cells with an abnormal chromosome complement, is clinically associated with pregnancy loss or congenital abnormalities. However, the transfer of diploid-aneuploid mosaic embryos can lead to healthy live births, indicating the early presence of aneuploid cells might not necessarily compromise embryo developmental competence. Currently, a comprehensive investigation of the molecular mechanisms determining post-implantation aneuploid cell fates is lacking. Here, we leverage cutting-edge human embryo extended culture techniques and single-cell omics to reveal global ploidy-specific transcriptomic signatures in post-implantation human embryos. We integrate cell demultiplexing and copy number variation calling to characterize diverse embryo cell populations. Differential expression and interactome analyses reveal that different intra-embryo environments facilitate distinct gene expression patterns related to energy expenditure, proteostasis, and DNA damage repair in the aneuploid cells. We observe temporary adaptive stress responses in homogeneous aneuploid embryos, whereas key machinery contributing to cell fitness is suppressed in aneuploid cells from mosaic embryos. Our findings point to a diminished survival advantage in aneuploid cells within post-implantation mosaic embryos, leading to self-correction by aneuploid cell depletion.

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“…The human thyroid gland, which is located at the base of the neck and wraps around the trachea just below the cricoid cartilage (1,2), is a very important endocrine organ, responsible for secreting thyroid hormones to maintain normal functioning of the body. Thyroxine (T4) and triiodothyronine (T3) play extremely important roles in protein synthesis, temperature regulation, and energy generation and regulation (3)(4)(5). Part of the T3 and T4 synthesized by and secreted from the thyroid gland combines with protein in the blood and is preserved temporarily, and the rest circulates as free forms known as FT3 and FT4, which are ready at any time to play their roles (6,7).…”

Section: Introductionmentioning

confidence: 99%

Comparison on the consistency of Mindray and Siemens chemiluminescence analyzers for detecting FT3, FT4 and TSH in patients with hyper- and hypothyroidism

Feng¹,

Wang²,

Zhang³

et al. 2022

Ann Transl Med

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Background: To examine the consistency of the Mindray and Siemens full-automatic chemiluminescence analyzers in detecting serum free triiodothyronine (FT3), free thyroxine (FT4) and thyroid stimulating hormone (TSH) in patients with hyper-or hypothyroidism.Methods: Included in this study were 164 new patients with abnormal thyroid function assessed at Nan Fang Hospital of Southern Medical University in 2021: 107 patients with hyperthyroidism and 57 with hypothyroidism. Additional 100 healthy individuals comprised the control group. Consistency of the FT3, FT4 and TSH results from the two systems in the different groups was analyzed by Kappa, linear correlation, regression, Bland-Altman and area under the receiver operating characteristic curve (AUC).Results: Compared with the control group, FT3 and FT4 levels were increased and TSH level was decreased in the hyperthyroid group (both P<0.05), whereas FT3 and FT4 levels were decreased and TSH level was increased in the hypothyroidism group (both P<0.05), suggesting that both test systems could provide references for relevant clinical evaluation. Kappa analysis showed high consistency of the two systems in detecting FT3, FT4 and TSH in both hyper-and hypothyroidism patients (mean Kappa value >0.7). In addition, there was a good linear correlation between the test results of the two systems (R 2 >0.90).Bland-Altman consistency analysis showed that most mean difference values were within the consistency limit (x±1.96s), indicating that the difference between the two systems was acceptable. AUC of FT3, FT4 and TSH detected by the two systems was higher than 0.80 in each group, showing no significant difference between them (both P>0.05), and the difference in sensitivity and specificity was within the acceptable range.Conclusions: Mindray and Siemens chemiluminescence analyzers are highly consistent in detecting FT3, FT4 and TSH in both hyper-and hypothyroidism patients.

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Thyroid hormone enhances stem cell maintenance and promotes lineage-specific differentiation in human embryonic stem cells

Cited by 8 publications

References 55 publications

VEGF Expression in Umbilical Cord MSC Depends on the Patient’s Health, the Week of Pregnancy in Which the Delivery Took Place, and the Body Weight of the Newborn – Preliminary Report

VEGF Expression in Umbilical Cord MSC Depends on the Patient’s Health, the Week of Pregnancy in Which the Delivery Took Place, and the Body Weight of the Newborn – Preliminary Report

Single-cell Transcriptomics of Postimplantation Embryos: Unveiling Aneuploidy Effects and Lineage Dynamics

Comparison on the consistency of Mindray and Siemens chemiluminescence analyzers for detecting FT3, FT4 and TSH in patients with hyper- and hypothyroidism

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