Thyrocytes, but not C cells, actively undergo growth and folliculogenesis at the periphery of thyroid tissue fragments in three-dimensional collagen gel culture

Toda, Shuji; Aoki, Shigehisa; Suzuki, Koichi; Koike, Eisuke; Ootani, Akifumi; Watanabe, Keiko; Koike, Norimasa; Sugihara, Hajime

doi:10.1007/s00441-003-0718-0

Cited by 25 publications

(18 citation statements)

References 29 publications

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“…7B) takes place at the peripheral zone, while the phenomena does not occur at the central zone, using three dimensional collagen gel culture of thyroid tissue fragments. 27,28 Secondly, we explain a "niche theory." That is, mature adipocytes, which are concentrated in the central zone of adipose tissue fragments, may organize a niche-like microenvironment for preadipocytes and MSCs, while the microenvironment may be lost at the peripheral zone of the tissue fragments due to the loss of mature adipocytes.…”

Section: Organotypic Culture As a Model For Tissue Regeneration And Omentioning

confidence: 99%

Adipose tissue-organotypic culture system as a promising model for studying adipose tissue biology and regeneration

et al. 2009

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Adipose tissue consists of mature adipocytes, preadipocytes and mesenchymal stem cells (MSCs), but a culture system for analyzing their cell types within the tissue has not been established. We have recently developed "adipose tissue-organotypic culture system" that maintains unilocular structure, proliferative ability and functions of mature adipocytes for a long term, using three-dimensional collagen gel culture of the tissue fragments. In this system, both preadipocytes and MSCs regenerate actively at the peripheral zone of the fragments. Our method will open up a new way for studying both multiple cell types within adipose tissue and the cell-based mechanisms of obesity and metabolic syndrome. Thus, it seems to be a promising model for investigating adipose tissue biology and regeneration. In this article, we introduce adipose tissue-organotypic culture, and propose two theories regarding the mechanism of tissue regeneration that occurs specifically at peripheral zone of tissue fragments in vitro.

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Section: Organotypic Culture As a Model For Tissue Regeneration And Omentioning

confidence: 99%

Adipose tissue-organotypic culture system as a promising model for studying adipose tissue biology and regeneration

et al. 2009

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“…In two-dimensional culture systems, thyrocytes may also lose normal functions such as TSH responsiveness (10). Culture systems with three-dimensional matrices or other growth factors can now recreate thyroid follicles in vitro and provide additional information on tumor-stromal interaction (11)(12)(13). A more recent concern raised by Schweppe et al is that many of the existing thyroid cell lines are redundant or are not thyroid in origin (14).…”

Section: Introductionmentioning

confidence: 99%

Lessons from Mouse Models of Thyroid Cancer

Kim

Zhu

2009

Thyroid

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Background: Thyroid cancer is the most common endocrine tumor and is increasing in incidence. The aim of this study was to review mouse models of differentiated thyroid cancer and how they elucidate human thyroid cancer biology. Summary: Differentiated thyroid cancer, primarily papillary and follicular, comprises the majority of thyroid cancers. There has been tremendous growth in the cross-talk between basic science and clinical practice for thyroid cancer management. Insight into the framework of genes responsible for differentiated thyroid cancer has been gained through the use of mouse models. Common genetic alterations found in human papillary thyroid cancer such as RET=PTC rearrangements or the BRAF V600E mutation have genetically modified mouse counterparts. These and other preclinical mouse models have validated the importance of the cyclic adenosine monophosphate (cAMP)=protein kinase A and mitogen-activated protein kinase (MAPK) signaling pathways in papillary thyroid cancer (PTC). RAS mutations have a role in both papillary and follicular thyroid cancer development. Mice with overactivation of the phosphatidylinol-3-kinase (PI3K)-AKT and=or thyrotropinregulated signaling pathways have been found to develop follicular thyroid cancer. Additional mouse models of thyroid cancer that utilize inducible expression systems are in development or are being characterized and will better reflect the majority of human thyroid cancers which are non-hereditary. Advances in in vivo imaging of mice allow for earlier detection of metastasis and the ability to follow tumor growth or regression which may be used in evaluation of pharmaceutical agents. Conclusions: Mouse models have expanded our understanding of the altered signaling pathways that contribute to thyroid cancer tumorigenesis and provide a powerful tool to develop novel diagnostic approaches and therapies.

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“…Toda et al in 2002 established new organotypic culture with the use of 3D collagen gel culture of thyroid tissue fragments with improved oxygenation through air exposure [5]. These models, by recreating thyroid follicles in vitro, give the opportunity to study information about tumour-stromal interactions [6]. However, when working with cell lines their uniqueness or derivation from the original tumour should be verified.…”

Section: Prace Poglądowementioning

confidence: 99%

“…powietrzu, pozwoliło na odtworzenie pęcherzyków tarczycowych, a tym samym analizę interakcji guzpodścielisko [5,6]. Praca z 2008 roku autorów Schweppe i wsp.…”

Section: Prace Poglądoweunclassified

Mysie modele raka brodawkowatego tarczycy — krótki przegląd

Rusinek

Krajewska

Jarząb

2016

Endokrynologia Polska

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Thyroid carcinoma (TC) is the most common endocrine malignancy, and its frequency is still rising. Papillary thyroid carcinoma (PTC) accounts for 80% of all TCs and usually is related to a very good prognosis. However, the standard therapeutic approaches are not always sufficient and disease progression is sometimes observed. These data highlight the limitation of our understanding of molecular mechanisms underlying tumorigenesis and how they vary between individual patients. Over the last 19 years mouse models of thyroid cancers have been developed in order to give answers to questions about their genetic background, relations of key molecular events with pathways fundamental for cancer, and many others. Among these models genetically engineered mice were of utmost importance regarding the input of knowledge about human tumorigenesis. In the present review the most significant mouse models of PTC are described with particular emphasis on BRAFV600E-induced ones, for the sake of its frequency in PTC, relation to factors of poor prognosis, and the fact that, since its identification, it became an attractive target in novel therapies. For the presented mouse models phenotype consequences of particular genetic alterations are described as well as the limitations of the used methods. StreszczenieRak tarczycy (TC) stanowi większość nowotworów złośliwych układu endokrynnego. Obserwuje się stały przyrost zachorowalności na TC, szczególnie na raka brodawkowatego tarczycy (PTC), który stanowi aż 80% wszystkich TC. Rak brodawkowaty tarczycy wiąże się najczęściej z bardzo dobrym rokowaniem. Zdarzają się jednak chorzy, w przypadku których standardowe leczenie okazuje się niewystarczające i obserwuje się progresję choroby, co podkreśla ograniczenia naszego zrozumienia mechanizmów molekularnych leżących u podłoża raka, jak również indywidualnych różnic. Przez ostatnich 19 lat zostało opracowanych wiele mysich modeli raka tarczycy, w celu odpowiedzi na pytania dotyczące jego podłoża genetycznego, związku kluczowych zmian genetycznych z fundamentalnymi dla raka ścieżkami sygnałowymi oraz wiele innych aspektów. Ze względu na wkład w poznanie mechanizmów ludzkiej kancerogenezy największe znaczenie odegrały modele oparte o genetyczne modyfikacje. W poniższym przeglądzie przybliżone zostały najważniejsze mysie modele raka brodawkowatego tarczycy. Najwięcej uwagi poświęcono modelom PTC indukowanym mutacją BRAFV600E w związku z największą częstością tej zmiany w PTC, jej związkiem z gorszym rokowaniem oraz faktem, że od momentu odkrycia stała się jednym z bardziej atrakcyjnych celów w nowych terapiach. Dla prezentowanych mysich modeli PTC podano konsekwencje fenotypowe poszczególnych zmian genetycznych, jak również ograniczenia wynikające z zastosowanej metody.

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Thyrocytes, but not C cells, actively undergo growth and folliculogenesis at the periphery of thyroid tissue fragments in three-dimensional collagen gel culture

Cited by 25 publications

References 29 publications

Adipose tissue-organotypic culture system as a promising model for studying adipose tissue biology and regeneration

Adipose tissue-organotypic culture system as a promising model for studying adipose tissue biology and regeneration

Lessons from Mouse Models of Thyroid Cancer

Mysie modele raka brodawkowatego tarczycy — krótki przegląd

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