Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity

Oliveira, Jonatas Rafael de; Viegas, Daiane de Jesus; Martins, Ana Paula Réquia; Carvalho, Cláudio Antônio Talge; Soares, Cristina Pacheco; Camargo, Samira Esteves Afonso; Jorge, Antônio Olavo Cardoso; Oliveira, Luciane Dias de

doi:10.1016/j.archoralbio.2017.06.031

Cited by 52 publications

(36 citation statements)

References 49 publications

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“…It is a flavoring for many meat-based foods. Its essential oil was shown to have antimicrobial, and anti-inflammatory properties [40]. According to our results, the thyme micelles and aqueous extract presented comparable effect with oregano oil, occupying the second place for antibacterial effect.…”

Section: Discussionmentioning

confidence: 58%

Antimicrobial Activity of Six Essential Oils Against a Group of Human Pathogens: A Comparative Study

et al. 2019

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Essential oils are concentrated natural extracts derived from plants, which were proved to be good sources of bioactive compounds with antioxidative and antimicrobial properties. This study followed the effect of some commonly used essential oils in micellar and aqueous extract on some of the most common pathogenic bacteria. Frankincense, myrtle, thyme, lemon, oregano and lavender essential oils were tested against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. Both micellar and aqueous extracts were used for determination of their minimal inhibitory (MIC) and bactericidal concentrations (MBC). The most active oils were oregano, thyme, lemon and lavender, while the least active were frankincense and myrtle. Oregano oil presented up to 64 times lower MICs/MBCs than ethylic alcohol, if considered as standard, on all bacteria. Most susceptible bacteria were the Gram-positive cocci, including methicillin resistant S. aureus, while the most resistant was P. aeruginosa. With some exceptions, the best activity was achieved by micelles suspension of essential oils, with MICs and MBCs ranging from 0.1% to > 50% v/v. Only oregano and lavender aqueous extracts presented bactericidal activity and only on K. pneumoniae (MIC = 6.3%). Thyme, lemon and oregano oils present significantly lower overall average MICs for their micellar form than for their aqueous extracts. The present results may suggest some formulas of colloid or micelle suspensions of whole essential oils such as oregano, thyme or lemon oil, that may help in antimicrobial fight. Aqueous extracts of oregano or thyme oil with good antibacterial activity could also be used in selected cases.

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Section: Discussionmentioning

confidence: 58%

Antimicrobial Activity of Six Essential Oils Against a Group of Human Pathogens: A Comparative Study

et al. 2019

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“…We have chosen the essential oils of confirmed biological activity, including eucalyptus oil (Eucalyptus globulus Labill. ), which exhibits antibacterial, antifungal, analgesic, and anti-inflammatory properties and has also been widely used in pharmaceutical, food, and cosmetics products [12]; thyme oil (Thymus vulgaris L.), which is known for its anti-inflammatory and antibacterial properties [13], and tea tree oil (Melaleuca alternifolia Cheel. ), which is employed largely for its antimicrobial properties and incorporated as an active ingredient in many topical formulations used to treat cutaneous infections [14].…”

Section: Introductionmentioning

confidence: 99%

The Novel Quantitative Assay for Measuring the Antibiofilm Activity of Volatile Compounds (AntiBioVol)

et al. 2020

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Herein, we present a new test, dubbed AntiBioVol, to be used for the quantitative evaluation of antibiofilm activity of volatile compounds in vitro. AntiBioVol is performed in two 24-well plates using a basic microbiological laboratory equipment. To demonstrate AntiBioVol usability, we have scrutinized the activity of volatilized eucalyptus, tea tree, thyme essential oils, and ethanol (used for method suitability testing) against biofilms of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. We have also compared AntiBioVol with the standard disc volatilization method, placing a special stress on evaluating the impact of various technical parameters on the outcomes of the latter method. The obtained results indicate that AntiBioVol allows analyzing the antibiofilm activity of volatile compounds in a high number of repeats and provides semi-quantitative or quantitative results of high repeatability. In comparison to disc volatilization, AntiBioVol is a more space- and cost-effective method that allows analyzing various types of microbial aggregates. Moreover, we have indicated that the possible reasons for the discrepancies in the results obtained by means of the standard disc volatilization method may be related to various parameters of the testing dishes used (height, volume, diameter) and to various volumes of the agar medium applied. In turn, the application of a 24-well plate and a strictly defined AntiBioVol protocol provide a higher control of experimental conditions. Therefore, the application of AntiBioVol may enable an optimization of and introduction of volatile compounds to the fight against infective biofilms.

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“…Besides, an hydroalcoholic extract of T. longicaulis (rich in rosmarinic acid) was shown to effectively inhibit COX-2 gene expression in THP-1 macrophages [42] and the aqueous extract of T. vulgaris were attested to decrease the release of the cytokine IL-8 for both H 2 O 2 or TNFα-stimulated peripheral blood lymphocytes (PBL) [71]. In addition, a commercial propylene glycol extract of T. vulgaris was reported to inhibit the production of cytokines IL-1β and TNF-α in LPS-activated RAW 264.7 macrophages after exposure to different concentrations of propylene glycol extracts [72].…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-inflammatory and Antitumoral Properties

2020

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Thymus genus comprises numerous species that are particularly abundant in the West Mediterranean region. A growing body of evidence suggests that many of these species are a rich source of bioactive compounds, including phenolic compounds such as rosmarinic acid, salvianolic acids and luteolin glycosides, able to render them potential applications in a range of industrial fields. This review collects the most relevant studies focused on the antioxidant, anti-inflammatory and anti-cancer of phenolic-rich extracts from Thymus plants, highlighting correlations made by the authors with respect to the main phenolic players in such activities.

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Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity

Abstract: T. vulgaris L. extract was effective against all biofilms, promoted high cell viability, anti-inflammatory effect and presented no genotoxicity.

Cited by 52 publications

References 49 publications

Antimicrobial Activity of Six Essential Oils Against a Group of Human Pathogens: A Comparative Study

Antimicrobial Activity of Six Essential Oils Against a Group of Human Pathogens: A Comparative Study

The Novel Quantitative Assay for Measuring the Antibiofilm Activity of Volatile Compounds (AntiBioVol)

Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-inflammatory and Antitumoral Properties

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