The Novel Quantitative Assay for Measuring the Antibiofilm Activity of Volatile Compounds (AntiBioVol)

Brożyna, Malwina; Żywicka, Anna; Fijałkowski, Karol; Gorczyca, Damian; Oleksy-Wawrzyniak, Monika; Dydak, Karolina; Migdał, Paweł; Dudek, Barbara; Bartoszewicz, Marzenna; Junka, Adam

doi:10.3390/app10207343

Cited by 7 publications

(13 citation statements)

References 39 publications

(45 reference statements)

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“…As a preliminary step in our investigation line, the disc diffusion method was used to screen the efficacy of all essential oils against all scrutinized pathogens. While this method has many disadvantages for the evaluation of liposoluble substances and may lead to biased outcomes, it is still commonly used as the primary screening method of antimicrobial activity of different substances, including essential oils [ 15 , 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, such factors, such as the method of EO extraction and preservation, are crucial for the content of their active substances [ 13 , 14 ]. Further, as we have indicated in our previous research, there are also significant discrepancies within EOs’ testing methods, translating into high deviations in outcomes [ 15 ].…”

Section: Introductionmentioning

confidence: 92%

“…AntiBioVol assay was conducted according to the protocol provided by Brożyna et al [15]. Two milliliters of agar (# 1) was poured into the wells of a 24-well plate and left to solidify.…”

Section: Antibiovol Measurement Of Antibiofilm Activity Of Volatile Compoundsmentioning

confidence: 99%

“…It was found that the antimicrobial activity of EOs is a result of a synergistic action of not only EO’s main components, but also of these, which occur in a low concentration of the specific EO. Another issue to address is the fact that EOs consist of volatile and liquid fractions, both of which display antimicrobial activity, although of different intensities [ 15 , 17 ]. The ability of specific EOs to eradicate biofilm or inhibit its formation has been reported as well, but how they could be applied in specific treatments against biofilm-based infections still requires elucidation [ 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the aim of this study was to compare antimicrobial (antibiofilm) activity of liquid and volatile fractions of specific essential oils against the above-described microorganisms in vitro, using various methodological approaches, since the final results often depend on the chosen method [ 15 , 41 , 42 ]. Special stress was put on biofilms formed on hydroxyapatite discs, which to some extent reflects the bone structure and to emphasize different absorption of volatile compounds by two types of porous material: agar and hydroxyapatite.…”

Section: Introductionmentioning

confidence: 99%

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Activity of Liquid and Volatile Fractions of Essential Oils against Biofilm Formed by Selected Reference Strains on Polystyrene and Hydroxyapatite Surfaces

et al. 2021

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Biofilms are surface-attached, structured microbial communities displaying higher tolerance to antimicrobial agents in comparison to planktonic cells. An estimated 80% of all infections are thought to be biofilm-related. The drying pipeline of new antibiotics efficient against biofilm-forming pathogens urges the search for alternative routes of treatment. Essential Oils (EOs), extracted from medicinally important plants, are a reservoir of bioactive compounds that may serve as a foothold in investigating novel antibiofilm compounds. The aim of this study was to compare antimicrobial activity of liquid and volatile fractions of tested EOs against biofilm-forming pathogens using different techniques. In this research, we tested five EOs, extracted from Syzygium aromaticum L., Boswelia serrata Roxb., Juniperus virginiana L., Pelargonium graveolens L. and Melaleuca alternifolia Cheel., against planktonic and biofilm forms of five selected reference strains, namely Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. To obtain cohesive results, we applied four various methodological approaches: to assess the activity of the liquid fraction of EOs, disc diffusion and the microdilution method were applied; to test EOs’ volatile fraction, the AntiBioVol assay and modified Antibiofilm Dressing Activity Measurement (A.D.A.M.) were used. The molecular composition and dynamics of antimicrobial substances released from specific EOs was measured using Gas Chromatography–Mass Spectrometry (GC-MS). The antimicrobial potency of EO’s volatile fraction against biofilm formed by tested strains differed from that of the liquid fraction and was related to the molecular weight of volatile compounds. The liquid fraction of CW-EO and volatile fraction of F-EO acted in the strongest manner against biofilm of C. albicans. The addition of 0.5% Tween 20 to liquid phase, enhanced activity of G-EO against E. coli and K. pneumoniae biofilm. EO activity depended on the microbial species it was applied against and the chosen assessment methodology. While all tested EOs have shown a certain level of antimicrobial and antibiofilm effect, our results indicate that the choice of EO to be applied against a specific biofilm-forming pathogen requires careful consideration with regard to the above-listed aspects. Nevertheless, the results presented in this research contribute to the growing body of evidence indicating the beneficial effects of EOs, which may be applied to fight biofilm-forming pathogens.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

“…AntiBioVol assay was conducted according to the protocol provided by Brożyna et al [15]. Two milliliters of agar (# 1) was poured into the wells of a 24-well plate and left to solidify.…”

Section: Antibiovol Measurement Of Antibiofilm Activity Of Volatile Compoundsmentioning

confidence: 99%