Thymus-Dependence and Kinetics of Intestinal Mast Cells and Globule Leucocytes

Ruitenberg, E. J.; Elgersma, Anneke; Lamers, Cor H.J.

doi:10.1007/978-1-4615-9101-6_113

Cited by 9 publications

(11 citation statements)

References 4 publications

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“…The latter two cell types are clearly different from the connective tis sue mast cells. The mucosal mast cell is similar in morphology to the metachromatically staining muco sal mast cells in rats [8,12] and mice [10], Consequently, we observed also in the human in testine a population of mucosal mast cells clearly dif ferent in morphology from the connective tissue mast cells. Their visualization with the FA fixative can probably be explained by a different chemical com position.…”

Section: Discussionmentioning

confidence: 52%

Effect of Fixation on the Light Microscopical Visualization of Mast Cells in the Mucosa and Connective Tissue of the Human Duodenum

Ruitenberg

Gustowska²,

Elgersma

et al. 1982

Int Arch Allergy Immunol

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Mast cells have been studied in the mucosa and connective tissue of 28 biopsies of human duodenum. They were collected from individuals who for various reasons had to undergo upper intestinal endoscopy. Tissues were fixed in both standard formalin and a formalin-acetic acid (FA) fixative developed for visualization of mucosal mast cells. With both fixatives connective tissue mast cells (CTMC) were equally well recognized. Only with the FA fixative, an additional population of mast cells was observed both in the mucosa and the connective tissue. This population consisted of round cells, always smaller than CTMC, with a number of granules staining violet with toluidine blue. These mast cells comprised two cell types, differing in size, the larger being present in both the mucosa and the connective tissue, the smaller ones in a subepithelial position in the crypts of Lieberkühn and in the adjacent submucosa. It was suggested that this first mentioned additional population was similar to the mucosal mast cells (MMC) observed in rats and mice. The subepithelially located metachromatic cells differed in morphology from the globule leukocytes found in rats and mice. It was concluded that in the human duodenum a population of cells with MMC staining characteristics is present, which can only be visualized under special fixation conditions. Their further characterization and role in various disease conditions remains to be elucidated.

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Section: Discussionmentioning

confidence: 52%

Effect of Fixation on the Light Microscopical Visualization of Mast Cells in the Mucosa and Connective Tissue of the Human Duodenum

Ruitenberg

Gustowska²,

Elgersma

et al. 1982

Int Arch Allergy Immunol

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“…Furthermore, the capacity of intestinal mas t cells to home toward the gut is under the influence of the thymus, but mast cells are probably derived from the bone marrow [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Tissue histamine levels in male and female normal and nude mice

et al. 1982

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In comparison with those in normal (+/+) mice, tissue histamine levels were lower in athymic (nu/nu) female mice and higher in athymic male mice. The sexual difference was less marked or absent in nu/nu mice. These results show (a) that endocrine factors are involved in the distribution of tissue histamine, and (b) that the thymus cannot be considered as the main source of tissue histamine in pathogen-free mice.

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“…Tissue mast cells are degranulated by immunoglobulin E and antigen [5], Whether this is true for IMC as well has not been proven as yet, al though IgE was demonstrated on IMC both in N. brasiliensis infected rats [10] and in T. spiralis infected rats [17], Since the worm burden and thus presumably the amount of antigen in ATS-treated animals was higher than in infected animals not receiving ATS treatment, a possibly reduced IMC degranu lation could be due to lower quantities of specific IgE. However, we have no evidence for this.…”

Section: Discussionmentioning

confidence: 99%

Intestinal Mast Cells and Globule Leucocytes: Role of the Thymus on Their Presence and Proliferation during a <i>Trichinella spiralis</i> Infection in the Rat

Ruitenberg

Elgersma

Kruizinga

1979

Int Arch Allergy Immunol

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The effect of treatment with anti-thymocyte serum (ATS) or neonatal thymectomy with or without thymus reconstitution on the presence and proliferation of intestinal mast cells (IMC) and globule leucocytes (GL) was studied in the small intestine of the rat during and oral Trichinella spiralis infection. ATS exerted an initial transient inhibitory effect on the IMC response and a marked inhibitory effect on the GL response to an oral Trichinella infection. Neonatal thymectomy decreased numbers of IMC but not of GL in the non-infected rat. Thymus reconstitution restored IMC numbers, however, not to normal values. The IMC response to Trichinella was comparable in thymectomized, thymectomized and reconstituted and intact animals. In contrast, the degree of GL response to Trichinella depended mainly on the presence of a thymus. The combined results were interpreted in favour of the hypothesis that the origin of IMC is thymus (T)-dependent, and the response to Trichinella thymus independent. No evidence for the hypothesis that subepithelial IMC are precursors for the intra-epithelially located GL was found. The latter were regarded as an independent cell population, its origin being thymus independent, and its reaction to Trichinella mainly thymus dependent.

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Thymus-Dependence and Kinetics of Intestinal Mast Cells and Globule Leucocytes

Cited by 9 publications

References 4 publications

Effect of Fixation on the Light Microscopical Visualization of Mast Cells in the Mucosa and Connective Tissue of the Human Duodenum

Effect of Fixation on the Light Microscopical Visualization of Mast Cells in the Mucosa and Connective Tissue of the Human Duodenum

Tissue histamine levels in male and female normal and nude mice

Intestinal Mast Cells and Globule Leucocytes: Role of the Thymus on Their Presence and Proliferation during a <i>Trichinella spiralis</i> Infection in the Rat

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