Thymosin β4 coated nanofiber scaffolds for the repair of damaged cardiac tissue

Kumar, Arun; Patel, Arjun; Duvalsaint, Louise; Desai, Mehir; Marks, Edward D.

doi:10.1186/1477-3155-12-10

Cited by 22 publications

(6 citation statements)

References 26 publications

(37 reference statements)

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“…Cell therapies also have potential in cardiovascular medicine and, indeed, transplant of modified embryonic stem cells which over-express Tβ4 have been shown to have benefit in a rat model of myocardial infarction [ 22 ]. Additionally, Tβ4 has been used to facilitate the production of cardiac patches designed to replace damaged tissue following myocardial infarction [ 23 ]. Knowledge of which cells Tβ4 is important to during normal development will help to refine and enhance such cell-based therapies for clinical use.…”

Section: Discussionmentioning

confidence: 99%

Expression and localisation of thymosin beta-4 in the developing human early fetal heart

et al. 2018

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BackgroundThe objective of this study was to investigate the expression and localisation of thymosin β4 (Tβ4) in the developing human heart. Tβ4 is a cardioprotective protein which may have therapeutic potential. While Tβ4 is an endogenously produced protein with known importance during development, its role within the developing human heart is not fully understood. Elucidating the localisation of Tβ4 within the developing heart will help in understanding its role during cardiac development and is crucial for understanding its potential for cardioprotection and repair in the adult heart.MethodsExpression of Tβ4 mRNA in the early fetal human heart was assessed by PCR using both ventricular and atrial tissue. Fluorescence immunohistochemistry was used to assess the localisation of Tβ4 in sections of early fetal human heart. Co-staining with CD31, an endothelial cell marker, and with myosin heavy chain, a cardiomyocyte marker, was used to determine whether Tβ4 is localised to these cell types within the early fetal human heart.ResultsTβ4 mRNA was found to be expressed in both the atria and the ventricles of the early fetal human heart. Tβ4 protein was found to be primarily localised to CD31-expressing endothelial cells and the endocardium as well as being present in the epicardium. Tβ4-associated fluorescence was greater in the compact layer of the myocardial wall and the interventricular septum than in the trabecular layer of the myocardium.ConclusionsThe data presented illustrates expression of Tβ4 in the developing human heart and demonstrates for the first time that Tβ4 in the human heart is primarily localised to endothelial cells of the cardiac microvasculature and coronary vessels as-well as to the endothelial-like cells of the endocardium and to the epicardium.

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Section: Discussionmentioning

confidence: 99%

Expression and localisation of thymosin beta-4 in the developing human early fetal heart

et al. 2018

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“…Among these techniques, electrospinning has attracted numerous interests as a simple and effective method [ 3 ]. Many studies have reported that artificial scaffolds composed of biocompatible polymers and various biomaterials can be manufactured by electrospinning [ 3 - 8 ]. In particular, electrospun matrices, composed of various polymers, including polyesterurethane, poly(ε-caprolactone) and poly(hydroxybutyrate), coated with proteins or not, have been designed as potential artificial scaffolds for skeletal muscle regeneration [ 9 - 12 ].…”

Section: Introductionmentioning

confidence: 99%

Stimulated myoblast differentiation on graphene oxide-impregnated PLGA-collagen hybrid fibre matrices

et al. 2015

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Background Electrospinning is a simple and effective method for fabricating micro- and nanofiber matrices. Electrospun fibre matrices have numerous advantages for use as tissue engineering scaffolds, such as high surface area-to-volume ratio, mass production capability and structural similarity to the natural extracellular matrix (ECM). Therefore, electrospun matrices, which are composed of biocompatible polymers and various biomaterials, have been developed as biomimetic scaffolds for the tissue engineering applications. In particular, graphene oxide (GO) has recently been considered as a novel biomaterial for skeletal muscle regeneration because it can promote the growth and differentiation of myoblasts. Therefore, the aim of the present study was to fabricate the hybrid fibre matrices that stimulate myoblasts differentiation for skeletal muscle regeneration.ResultsHybrid fibre matrices composed of poly(lactic-co-glycolic acid, PLGA) and collagen (Col) impregnated with GO (GO-PLGA-Col) were successfully fabricated using an electrospinning process. Our results indicated that the GO-PLGA-Col hybrid matrices were comprised of randomly-oriented continuous fibres with a three-dimensional non-woven porous structure. Compositional analysis showed that GO was dispersed uniformly throughout the GO-PLGA-Col matrices. In addition, the hydrophilicity of the fabricated matrices was significantly increased by blending with a small amount of Col and GO. The attachment and proliferation of the C2C12 skeletal myoblasts were significantly enhanced on the GO-PLGA-Col hybrid matrices. Furthermore, the GO-PLGA-Col matrices stimulated the myogenic differentiation of C2C12 skeletal myoblasts, which was enhanced further under the culture conditions of the differentiation media.ConclusionsTaking our findings into consideration, it is suggested that the GO-PLGA-Col hybrid fibre matrices can be exploited as potential biomimetic scaffolds for skeletal tissue engineering and regeneration because these GO-impregnated hybrid matrices have potent effects on the induction of spontaneous myogenesis and exhibit superior bioactivity and biocompatibility.

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“…Also, the reduction in the size of MI and fibrosis was reported with an increase in scar thickness. PCL-based nanoscaffolds with thymosin β4 coating functionalized with murine-derived cardiomyocytes to improve cardiac functions were also reported [114].…”

Section: Nanofibersmentioning

confidence: 99%

Nanomaterials: A Promising Therapeutic Approach for Cardiovascular Diseases

Chopra

Bibi

Mishra

et al. 2022

Journal of Nanomaterials

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Cardiovascular diseases (CVDs) are a primary cause of death globally. A few classic and hybrid treatments exist to treat CVDs. However, they lack in both safety and effectiveness. Thus, innovative nanomaterials for disease diagnosis and treatment are urgently required. The tiny size of nanomaterials allows them to reach more areas of the heart and arteries, making them ideal for CVDs. Atherosclerosis causes arterial stenosis and reduced blood flow. The most common treatment is medication and surgery to stabilize the disease. Nanotechnologies are crucial in treating vascular disease. Nanomaterials may be able to deliver medications to lesion sites after being infused into the circulation. Newer point-of-care devices have also been considered together with nanomaterials. For example, this study will look at the use of nanomaterials in imaging, diagnosing, and treating CVDs.

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Thymosin β4 coated nanofiber scaffolds for the repair of damaged cardiac tissue

Cited by 22 publications

References 26 publications

Expression and localisation of thymosin beta-4 in the developing human early fetal heart

Expression and localisation of thymosin beta-4 in the developing human early fetal heart

Stimulated myoblast differentiation on graphene oxide-impregnated PLGA-collagen hybrid fibre matrices

Nanomaterials: A Promising Therapeutic Approach for Cardiovascular Diseases

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