Thymoquinone protects DRG neurons from axotomy-induced cell death

Üstün, Ramazan; Oğuz, Elif Kaval; Şeker, Ayşe; Korkaya, Hasan

doi:10.1080/01616412.2018.1504157

Cited by 10 publications

(5 citation statements)

References 43 publications

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“…Moreover, TQ alleviates the testicular damage in diabetic rats through its powerful antioxidant and hypoglycemic effects [11]. Additionally, TQ shows a regenerative potential for treating damaged peripheral nerves [17].…”

Section: Introductionmentioning

confidence: 99%

Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart

El‐Far

Elewa

Abdelfattah

et al. 2021

IJMS

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D-galactose (D-gal) administration causes oxidative disorder and is widely utilized in aging animal models. Therefore, we subcutaneously injected D-gal at 200 mg/kg BW dose to assess the potential preventive effect of thymoquinone (TQ) and curcumin (Cur) against the oxidative alterations induced by D-gal. Other than the control, vehicle, and D-gal groups, the TQ and Cur treated groups were orally supplemented at 20 mg/kg BW of each alone or combined. TQ and Cur effectively suppressed the oxidative alterations induced by D-gal in brain and heart tissues. The TQ and Cur combination significantly decreased the elevated necrosis in the brain and heart by D-gal. It significantly reduced brain caspase 3, calbindin, and calcium-binding adapter molecule 1 (IBA1), heart caspase 3, and BCL2. Expression of mRNA of the brain and heart TP53, p21, Bax, and CASP-3 were significantly downregulated in the TQ and Cur combination group along with upregulation of BCL2 in comparison with the D-gal group. Data suggested that the TQ and Cur combination is a promising approach in aging prevention.

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Section: Introductionmentioning

confidence: 99%

Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart

El‐Far

Elewa

Abdelfattah

et al. 2021

IJMS

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“…Recently, a study administered two doses of TQ, at 50 and 75 nM, in a PNI mouse model induced by laser microdissection. The results showed an increased survival of neurons isolated from DRG, increased SCs and fibroblast proliferation, and more extension of neurites in vitro , at 75 nM ( Üstün et al, 2018 ).…”

Section: Preclinical Studies Modeling Peripheral Neuropathies or Peri...mentioning

confidence: 99%

Neuron-Schwann cell interactions in peripheral nervous system homeostasis, disease, and preclinical treatment

Oliveira,

Yanick,

Wein

et al. 2023

Front. Cell. Neurosci.

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Schwann cells (SCs) have a critical role in the peripheral nervous system. These cells are able to support axons during homeostasis and after injury. However, mutations in genes associated with the SCs repair program or myelination result in dysfunctional SCs. Several neuropathies such as Charcot–Marie–Tooth (CMT) disease, diabetic neuropathy and Guillain–Barré syndrome show abnormal SC functions and an impaired regeneration process. Thus, understanding SCs-axon interaction and the nerve environment in the context of homeostasis as well as post-injury and disease onset is necessary. Several neurotrophic factors, cytokines, and regulators of signaling pathways associated with proliferation, survival and regeneration are involved in this process. Preclinical studies have focused on the discovery of therapeutic targets for peripheral neuropathies and injuries. To study the effect of new therapeutic targets, modeling neuropathies and peripheral nerve injuries (PNIs) in vitro and in vivo are useful tools. Furthermore, several in vitro protocols have been designed using SCs and neuron cell lines to evaluate these targets in the regeneration process. SCs lines have been used to generate effective myelinating SCs without success. Alternative options have been investigated using direct conversion from somatic cells to SCs or SCs derived from pluripotent stem cells to generate functional SCs. This review will go over the advantages of these systems and the problems associated with them. In addition, there have been challenges in establishing adequate and reproducible protocols in vitro to recapitulate repair SC-neuron interactions observed in vivo. So, we also discuss the mechanisms of repair SCs-axon interactions in the context of peripheral neuropathies and nerve injury (PNI) in vitro and in vivo. Finally, we summarize current preclinical studies evaluating transgenes, drug, and novel compounds with translational potential into clinical studies.

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“…Recently, cultures of neuronal and glial cells exposed to multiple 6-nanosecond laser pulses at 355 nm exhibited immediate partial retraction of axons from the site of laser-induced injury, followed by axonal beading and proximal segment retraction after 1 s [ 67 ]. Interestingly, cell bodies of neurons that were subjected to laser-induced axonal injury demonstrated numerous vacuoles [ 68 ], especially in the mitochondria [ 69 ]. Similarly, neurite injury induced by a UV laser beam resulted in proximal and distal segment retraction with signs of degeneration comparatively higher in thicker segments than thinner segments [ 68 , 70 ].…”

Section: Transection Injury Modelsmentioning

confidence: 99%

“…Interestingly, cell bodies of neurons that were subjected to laser-induced axonal injury demonstrated numerous vacuoles [ 68 ], especially in the mitochondria [ 69 ]. Similarly, neurite injury induced by a UV laser beam resulted in proximal and distal segment retraction with signs of degeneration comparatively higher in thicker segments than thinner segments [ 68 , 70 ]. In another laser-based transection model, low-energy picosecond laser pulses were used to induce single axon transection in vitro, with micro-level resolution [ 71 ] ( Figure 3 ).…”

Section: Transection Injury Modelsmentioning

confidence: 99%

A Brief Review of In Vitro Models for Injury and Regeneration in the Peripheral Nervous System

Varier

Raju

Madhusudanan

et al. 2022

IJMS

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Nerve axonal injury and associated cellular mechanisms leading to peripheral nerve damage are important topics of research necessary for reducing disability and enhancing quality of life. Model systems that mimic the biological changes that occur during human nerve injury are crucial for the identification of cellular responses, screening of novel therapeutic molecules, and design of neural regeneration strategies. In addition to in vivo and mathematical models, in vitro axonal injury models provide a simple, robust, and reductionist platform to partially understand nerve injury pathogenesis and regeneration. In recent years, there have been several advances related to in vitro techniques that focus on the utilization of custom-fabricated cell culture chambers, microfluidic chamber systems, and injury techniques such as laser ablation and axonal stretching. These developments seem to reflect a gradual and natural progression towards understanding molecular and signaling events at an individual axon and neuronal-soma level. In this review, we attempt to categorize and discuss various in vitro models of injury relevant to the peripheral nervous system and highlight their strengths, weaknesses, and opportunities. Such models will help to recreate the post-injury microenvironment and aid in the development of therapeutic strategies that can accelerate nerve repair.

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Thymoquinone protects DRG neurons from axotomy-induced cell death

Abstract: TQ exhibits a regenerative potential for the treatment of damaged peripheral nerves.

Cited by 10 publications

References 43 publications

Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart

Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats’ Brain and Heart

Neuron-Schwann cell interactions in peripheral nervous system homeostasis, disease, and preclinical treatment

A Brief Review of In Vitro Models for Injury and Regeneration in the Peripheral Nervous System

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