Thymoquinone and Oleuropein Combination Ameliorates Renal Ischemia-Reperfusion Injury by Attenuating Oxidative Stress in Rats

Abstract

Background and Objective: Ischemia-reperfusion-induced acute renal injury is a common clinical problem. The current article investigates the effects of oleuropein and thymoquinone combination in the prophylaxis of renal ischemia-reperfusion-induced injury in rats. Materials and Methods: Thirty rats were divided into five groups, G 1 : Sham operation group, G 2 : IR group, G 3 : IR+Thymoquinone (10 mg kgG 1 , p.o.) G 4 : IR+Oleuropein (50 mg kgG 1 , p.o.) and G 5 : IR-TQ (10 mg kgG 1 , p.o.)+OP (50 mg kgG 1 , p.o.). A pre-treatment was given for three weeks before IR surgery. The blood urea nitrogen (BUN), uric acid and serum creatinine were analyzed in serum. The homogenate of kidney tissues was prepared for the estimation of the anti-oxidant parameters like Glutathione Peroxidase (Gpx), Superoxide Dismutase (SOD), Catalase (CAT) and Malondialdehyde (MDA). Results: The rats in G 2 (IR) showed high levels of creatinine (4.11±0.18 mg dLG 1 ), BUN (131.76±10.56 mg dLG 1 ) and uric acid (1.72±0.14 mg dLG 1 ) as compared to G 1 (sham-operated rats). Further, the increased levels of MDA (10.54±0.77 nmol mgG 1 protein) and low levels of SOD (78.92±3.46 U mgG 1 protein), CAT (23.49±1.93 U mgG 1 protein) and GSH-Px (26.53±0.64 U mgG 1 Protein) activity was evidenced in the IR group. The combination treatment group G 5 (IR-TQ-OP) showed the best nephroprotective activity among all the treatment groups, as the kidneys were least affected by the IR-induced renal inflammation and oxidative injuries. It was followed by IR-TQ and then IR-OP. All these observations were corroborated by the findings of histopathological investigations. Conclusion: Simultaneous administration of TQ and OP significantly altered the experimentally induced renal function due to ischemia/reperfusion.

Effect of Taxifolin on Acrylamide-Related Oxidative Ovarian Damage, Infertility and Intrauterine Growth Retardation in Female Rats

“…Studies have shown that AA causes oxidative stress in organs and tissues and the severity of stress increases even more at high doses 24 . As is known, the balance between the formation and destruction of oxidants is important for the normal continuation of biological processes 25 . In current study, in accordance with the literature, it was observed that the amount of MDA in the ovarian tissues of animals increased significantly in the AA-administered group compared to the healthy and taxifolin-treated groups.…”