Effect of Taxifolin on Acrylamide-Related Oxidative Ovarian Damage, Infertility and Intrauterine Growth Retardation in Female Rats

Abstract

Background and Objective: Acrylamide (AA) is a toxin that can cause reproductive organ toxicity and infertility through oxidative stress.This study aimed to investigate the effect of taxifolin, an antioxidant flavonoid, against acrylamide-induced infertility and intrauterine growth disorder in rats. Materials and Methods: Thirty-six rats were randomly divided into HG, ACR and TACR groups. The TACR group was given orally 50 mg kgG 1 of taxifolin. One hour later, 20 mg kgG 1 acrylamide was administered orally to the ACR and TACR groups. This procedure was repeated once a day for 30 days. Afterward, 6 rats in the groups were euthanized with 50 mg kgG 1 thiopental sodium. The removed ovaries were analyzed biochemically and histopathologically. Others were kept in the laboratory with male rats for 2 months for breeding. Rats that did not become pregnant and did not give birth during this period were considered infertile. Intrauterine growth retardation was assessed by the weight of the offspring. Results: Taxifolin decreased the levels of malondialdehyde (MDA), total oxidant system (TOS) and oxidative stress index (OSI) that increased with acrylamide in ovarian tissues while preventing the decrease in Total Glutathione (tGSH) and total antioxidant system (TAS) levels. In the histopathological examination, it was observed that the damage in the ACR group decreased with the application of taxifolin. In addition, it was determined that it significantly prevented infertility and the decrease the birth weight of the offspring. Conclusion: Taxifolin may be a potential therapeutic strategy to prevent acrylamide-induced ovarian damage, infertility and intrauterine growth retardation.