Thymol and trans-cinnamaldehyde reduce active nutrient absorption and chloride secretion in the pig jejunal Ussing chamber model

Michiels, Joris; Missotten, Joris; Dierick, Noël; Fremaut, Dirk; Smet, Stefaan De

doi:10.1016/j.livsci.2010.06.087

Cited by 10 publications

(8 citation statements)

References 6 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the latter experiment, cinnamaldehyde and thymol did not affect TEER (Boudry and Perrier, 2008), whereas in the former, in contrast to results of our study, cinnamaldehyde increased resistance slightly (Michiels et al, 2010). The reason for this discrepancy in not known.…”

Section: Discussioncontrasting

confidence: 92%

“…The effects of thymol and cinnamaldehyde on intestinal tissue resistance have been studied by Michiels et al (2010) and Boudry and Perrier (2008). In the latter experiment, cinnamaldehyde and thymol did not affect TEER (Boudry and Perrier, 2008), whereas in the former, in contrast to results of our study, cinnamaldehyde increased resistance slightly (Michiels et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Intestinal fluid flux also increases with bacterial toxins -TEER declined on treatment with EHEC cell-free metabolites (Putaala et al, 2008). However, the amounts of cinnamaldehyde and thymol in Michiels et al (2010) were higher than those of standard animal feed, which might be the reason for the inconsistency.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Putaala¹,

Nurminen²,

Tiihonen³

2017

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Putaala¹,

Nurminen²,

Tiihonen³

2017

J. Anim. Feed Sci.

View full text Add to dashboard Cite

“…Thymol and vanillin are botanical compounds that have in vitro and in vivo anti-inflammatory and anti-oxidant activity and are widely used in animal nutrition [ 11 , 31 ]. The effects of thymol on intestinal secretory activity were studied ex vivo by Boudry and Perrier [ 32 ] and Michiels et al [ 33 ] demonstrated that thymol induces ion secretions in the small intestine via the nervous system. As electrolytes and water secretion is usually a means to eliminate bacterial toxins from the lumen, thymol may plays a role in the defense of the organism and consequently affects the barrier function.…”

Section: Discussionmentioning

confidence: 99%

Nature-Identical Compounds and Organic Acids Ameliorate and Prevent the Damages Induced by an Inflammatory Challenge in Caco-2 Cell Culture

Toschi

Rossi²,

Tugnoli³

et al. 2020

Molecules

View full text Add to dashboard Cite

Bioactive compounds, such as organic acids (OA) and nature-identical compounds (NIC), can exert a role in the protection of intestinal mucosa functionality due to their biological properties. The aim of this study was to understand the role of 2 OA (citric and sorbic acid) and 2 NIC (thymol and vanillin), alone or combined in a blend (OA + NIC), on intestinal barrier functionality, either during homeostatic condition or during an inflammatory challenge performed with pro-inflammatory cytokines and lipopolysaccharides (LPS). The study was performed on the human epithelial cell line Caco-2, a well-known model of the intestinal epithelial barrier. The results showed how OA and NIC alone can improve transepithelial electrical resistance (TEER) and mRNA levels of tight junction (TJ) components, but OA + NIC showed stronger efficacy compared to the single molecules. When an inflammatory challenge occurred, OA + NIC blend was able both to ameliorate, and prevent, damage caused by the pro-inflammatory stimulus, reducing or preventing the drop in TEER and improving the TJ mRNA expression. The data support the role of OA + NIC in modulating gut barrier functionality and reducing the negative effects of inflammation in intestinal epithelial cells, thereby supporting the gut barrier functionality.

show abstract

“…Segments of distal SI were used to measure intestinal permeability and ion transport with the Ussing chamber system according to Michiels, Missotten, Dierick, Fremaut, and De Smet () and Wang et al (). In brief, for each piglet, the segment (20 cm) was flushed with saline, then cut along the mesenteric border, stripped of the muscle layers and mounted in modified Ussing chambers on a segment holder, as flat sheets with an exposed tissue area of 1.07 cm 2 .…”

Section: Methodsmentioning

confidence: 99%

Effect of zinc oxide sources and dosages on gut microbiota and integrity of weaned piglets

Wang

Noten

Degroote

et al. 2018

Animal Physiology Nutrition

Self Cite

View full text Add to dashboard Cite

Zinc oxide (ZnO) supplied at pharmacological dosage in diets of weaned piglets improves growth performance. However, it causes environmental contamination and induces bacterial antibiotic resistance, yet this practice is debated. The effects on gut microbiota and integrity in weaned piglets of conventional ZnO at nutritional and pharmacological dosage (110 and 2,400 mg/kg Zn, respectively) were compared to an alternative ZnO source at 110 and 220 mg/kg Zn. Each of the four treatments was applied to four pens (two piglets/pen; weaning age, 20 days) for 15 days, and piglets were sampled on day 15 to determine indices of gut integrity. Feeding conventional ZnO at 2,400 mg/kg Zn reduced coliforms and Escherichia coli in distal small intestine as compared to conventional ZnO at 110 mg/kg (−1.7 and −1.4 log10 cfu/g, respectively), whereas the alternative ZnO reduced only coliforms, irrespective of dosage (−1.6 to −1.7 log10 cfu/g). Transepithelial electrical resistance of distal small intestinal mucosa was higher for pigs fed the alternative ZnO source as compared with groups fed 110 mg/kg Zn of conventional ZnO, in line with a trend for higher gene expression of claudin‐1 and zona occludens‐1. Interestingly, the alternative ZnO source at 110 and 220 mg/kg Zn increased intestinal alkaline phosphatase gene transcript as compared to conventional ZnO at 110 mg/kg Zn, whereas the alternative ZnO source at 110 mg/kg Zn exhibited higher Zn concentrations in mucosa (2,520 μg/g) as compared to conventional ZnO at 110 mg/kg Zn (1,211 μg/g). However, assessing alkaline phosphatase activity, no significant effects were found. In conclusion, the alternative ZnO reduced digesta Enterobacteriaceae numbers and improved gut integrity, albeit similar or better, depending on the dosage, to the effects of pharmacological dosage of conventional ZnO.

show abstract

Thymol and trans-cinnamaldehyde reduce active nutrient absorption and chloride secretion in the pig jejunal Ussing chamber model

Cited by 10 publications

References 6 publications

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Effects of cinnamaldehyde and thymol on cytotoxicity, tight junction barrier resistance, and cyclooxygenase-1 and -2 expression in Caco-2 cells

Nature-Identical Compounds and Organic Acids Ameliorate and Prevent the Damages Induced by an Inflammatory Challenge in Caco-2 Cell Culture

Effect of zinc oxide sources and dosages on gut microbiota and integrity of weaned piglets

Contact Info

Product

Resources

About